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Reusable glucose sensor as well as preparation method and application thereof

A glucose sensor and nano-cube technology, applied in the field of biosensors, can solve the problems of poor conductivity of glucose sensors, etc., and achieve the effects of excellent anti-interference, low glucose oxidation potential, and fast redox electron transfer rate

Active Publication Date: 2022-04-19
江苏跃凯生物技术有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a reusable glucose sensor and its preparation method and application to solve the problem of poor conductivity of the existing glucose sensor

Method used

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  • Reusable glucose sensor as well as preparation method and application thereof
  • Reusable glucose sensor as well as preparation method and application thereof
  • Reusable glucose sensor as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A kind of reusable glucose sensor, its preparation method comprises the following steps:

[0043] S1, add 24ml K 3 [Fe(CN) 6 ] solution (0.04M) was added dropwise to the solution containing 0.03M Ni(NO 3 ) 2 and 0.0375MC 6 h 5 Na 3 o 7 centrifuged at 4000r / min for 10 minutes, and finally dried at 75°C for 10 hours to obtain Ni-Fe PBA nanocube materials.

[0044] S2. Add 10mL of ammonia water (8.5wt%) dropwise to 5mL of Ni-Fe-PBA nanocube suspension (2mg / mL), and stir at room temperature for 10 minutes to prepare Ni-Fe-PBA nanocages. The prepared Ni-Fe-PBA nanocages were washed three times with deionized water and ethanol successively, and dried at 75° C. for 10 h to obtain a hollow Ni-Fe-PBA nanocage material.

[0045] S3, 1ml HAuCl 4 ·3H 2 O (10 mM) was added dropwise to 6 ml of 1 mg / mL Ni-Fe PBA nanocage suspension containing 20 mg Pluronic F127, stirred for 30 min, and then 1.4 ml of ascorbic acid (10 mM) was added to deposit AuNPs on Ni-Fe PBA in the nano...

experiment example 1

[0050] The Ni-FePBA nano-cubic material, the hollow Ni-Fe PBA nano-cage material and the Au@Ni-Fe PBA nano-cage material prepared in Example 1 were tested for microscopic morphology, and the test results are shown in figure 2 .

[0051] Depend on figure 2 a, d It can be seen that the Ni-Fe PBA nanocrystal has a solid cubic structure with a uniform size of about 140nm. Solid-state Ni-Fe PBA nanocubes were etched into hollow nanocages with spindle-shaped cavities ( figure 2 b,e). Gold nanoparticles (40-45nm) were formed by in situ reduction, and gold nanoparticles were embedded in Ni-FePBA nanocages ( figure 2 c, f).

experiment example 2

[0053] The Ni-FePBA nano-cube material, the hollow Ni-Fe PBA nano-cage material and the Au@Ni-Fe PBA nano-cage material prepared in Example 1 are tested for crystal form, the results are shown in image 3 .

[0054] Depend on image 3 It can be seen that in the XRD spectra of Ni-Fe PBA nanocubes, Ni-Fe PBA nanocages and Au@Ni-Fe PBA nanocages, there appeared a The peaks corresponding to the crystal planes indicate that the crystal structure of PBA is well maintained during the controlled etching and in situ gold deposition. The diffraction peaks of Au@Ni-Fe PBA nanocages at 38.18°, 44.41°, 64.51° and 77.61° belong to (111), (200), (220) and (311) crystals of Au (PDF#04-0784). surface, indicating that Au NPs were successfully coupled in Ni-Fe PBA nanocages.

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Abstract

The invention discloses a reusable glucose sensor as well as a preparation method and application thereof, and belongs to the technical field of biosensors. According to the method, controlled etching is carried out on the vertex of a nanocube of a lux blue simulant (PBA), and then Au nanoparticles (AuNPs) are embedded into an etched cavity through in-situ reduction of HAuCl4. The unique AuNP-PBA nano mosaic has low glucose oxidation potential, high electrocatalytic activity and high redox electron transfer rate. The non-enzymatic glucose sensor is formed by covalently immobilizing the AuNP-PBA nano mosaic on a fine gold wire, and has a particularly wide linear detection range (10 [mu] M to 16 mM), excellent anti-interference performance and quick response. More importantly, the sensor can be repeatedly used, and the sensitivity of the sensor is still kept good even after 150 times of detection.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to a reusable glucose sensor and its preparation method and application. Background technique [0002] Glucose detection is important in many applications. In the field of health care, there are more than 463 million diabetic patients worldwide, and blood glucose monitoring is currently the only way to reflect the patient's glucose metabolism level to manage diabetes and prevent serious complications such as macular degeneration, diabetic nephropathy and heart failure. In addition, glucose detection is also widely used in fields such as environmental monitoring and food quality control. [0003] Commonly used glucose detection methods rely on electrochemical detection test strips. Glucose dehydrogenase (GDH) or glucose oxidase (GOx) in the test strip reacts with the glucose in the sample to generate an electrical signal related to the glucose concentration. This method has rap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327C01C3/12B22F9/24B22F1/054B22F1/17
CPCG01N27/26G01N27/3278C01C3/12B22F9/24
Inventor 胡雪丰张婕妤王云兵
Owner 江苏跃凯生物技术有限公司
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