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Application of braA08g039212E gene derived from European turnip ECD04 in plasmodiophora brassicae resistance improvement

A technology of braa08g039212e and 1.braa08g039212e, which is applied in the field of gene cloning, can solve the problems of plant failure, affecting the growth and development of above-ground parts, and damage to the main root system, and achieve the effect of shortening the breeding process.

Pending Publication Date: 2022-04-22
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For plants seriously infested by Plasmodium fungus, the main root system may be completely destroyed, thereby affecting the growth and development of the aboveground parts, resulting in plant failure

Method used

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  • Application of braA08g039212E gene derived from European turnip ECD04 in plasmodiophora brassicae resistance improvement
  • Application of braA08g039212E gene derived from European turnip ECD04 in plasmodiophora brassicae resistance improvement
  • Application of braA08g039212E gene derived from European turnip ECD04 in plasmodiophora brassicae resistance improvement

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Embodiment 1

[0040] 1. Fine positioning group construction

[0041] Huashuang 5 (Brassica napus, AACC, 2n=38), a conventional variety of Brassica napus in China, was used as the recipient parent (also known as H5S), and European turnip ECD04 (Brassica rapa, AA, 2n=20) was used as the donor parent. After one crossing and multiple backcrossings, combined with molecular marker-assisted screening, the anti-clubber locus PbBa8.1 in ECD04 was introduced into Huashuang 5, and BC1, BC2 and BC3 materials were obtained. After 4 times of selfing, combined with molecular marker screening and field identification of disease resistance, the disease-resistant variety Huashuang 5R (also known as H5R) of Physiological race 4 of Plasmodium chinensis was successfully bred, and a BC3F4 population was obtained. This population showed a disease-resistant phenotype segregation in the field inoculation in the diseased area, so it was selected as a fine-mapping population.

[0042] The currently mapped interval i...

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Abstract

The invention discloses an application of a BraA08g039212E gene derived from European turnip ECD04 in plasmodiophora brassicae resistance improvement, and discloses a resistance effect of the BraA08g039212E gene derived from European turnip ECD04 on a Chinese plasmodiophora brassicae No.4 physiological race, a nucleotide sequence of the gene is shown as SEQ ID No.1 or SEQ ID No.2, and an amino acid sequence of the gene is shown as SEQ ID No.3. The method comprises the following steps: finely positioning the gene by utilizing a map-based cloning method; the physical position of the gene on an A08 chromosome is determined, and then the gDNA or cDNA of the gene is used for cloning and genetic transformation of rape which is not resistant to clubroot, so that the clubroot resistance function of the gene on plasmodiophora brassicae is verified. Scientific and technical bases can be provided for breeding new varieties of brassica anti-clubroot plants through a genetic engineering means or a molecular marker-assisted selection technology.

Description

technical field [0001] The invention relates to the technical field of gene cloning, in particular to the application of a BraA08g039212E gene derived from Brassica napus ECD04 in improving the resistance of Plasmodium tulipiformis. Background technique [0002] Clubroot is a soil-borne disease caused by Plasmodiophora brassicae Woronin, which seriously endangers the growth and development of cruciferous crops. Plasmodium exists in the form of dormant spores in the soil, and after germination, it reproduces in the host body in a biotrophic manner, which disrupts the metabolism of auxin and cytokinin in the host plant, resulting in swollen roots of the plant. For plants seriously infested by Plasmodium, the main root system may be completely destroyed, thereby affecting the growth and development of the aboveground parts, resulting in plant failure. Rapeseed is an important oil crop in my country, and its yield is deeply affected by Pluzobacteria. Clubroot is mainly distrib...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C12N15/11C07K14/415C12Q1/6895A01H5/00A01H6/20
CPCC07K14/415C12N15/8282C12Q1/6895C12Q2600/13
Inventor 张椿雨甘龙财申继成敦必成陈鹏
Owner HUAZHONG AGRI UNIV
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