Conductive nano-material glucose sensing material as well as preparation method and application thereof

A technology of conductive nano and sensing materials, which is applied in the direction of material analysis, analysis materials, instruments, etc. by electromagnetic means, can solve the problems of reduced sensitivity and weakened anti-interference ability, and increase the rate of transferred electrons and linear correlation coefficient High, the effect of overcoming the oxygen effect

Active Publication Date: 2022-04-22
江苏跃凯生物技术有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the above-mentioned problems in the prior art, the present invention provides a special material for glucose intelligent monitoring and its application, so as to solve the problem of reduced sensitivity and anti-interference ability caused by the interference of oxygen in the process of detecting glucose by the existing second-generation CGMS wired enzyme technology. Weak and other issues

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  • Conductive nano-material glucose sensing material as well as preparation method and application thereof
  • Conductive nano-material glucose sensing material as well as preparation method and application thereof
  • Conductive nano-material glucose sensing material as well as preparation method and application thereof

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Embodiment 1

[0032] This embodiment provides a redox ligand-based conductive nanomaterial glucose sensing material, the preparation process of which includes the following steps:

[0033] A. Weigh 1-100mg of multi-walled carbon nanotubes into a 10mL vial, add 1-3mL of deionized water and 0.01-1mL of 3-aminopropyltriethoxysilane, and sonicate for 10-100min to obtain a uniform dispersion solution, placed at 25-50°C for 1-24 hours, 0.1-100 μL of 0.1M hydrochloric acid was added to it, placed at room temperature for 6-48 hours, the obtained suspension was dialyzed, and the dialyzed suspension was washed by centrifugation several times to obtain carbon with amino groups on the surface. nanotube.

[0034] B. Mix 10~100mg Os(bpy) 2 ClIm(CH 2 ) 11 -COOH was fully dissolved in PBS with pH=5~8 under stirring, then 150mg 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and 200mg N-hydroxysuccinimide were added Amine, stirred at room temperature for 15-60min. 100 μL of aminated carbon nano...

Embodiment 2

[0037] This embodiment provides a redox ligand-based conductive nanomaterial glucose sensing material, the preparation process of which includes the following steps:

[0038] A. Weigh 1-100mg of graphene into a 10mL vial, add 1-3mL of deionized water and 0.01-1mL of methacryloxypropyltrimethoxysilane, and sonicate for 10-100min to obtain a uniform dispersion solution, placed at 25-50°C for 1-24 hours, 0.1-100 μL of 0.1M hydrochloric acid was added to it, left at room temperature for 6-48 hours, the obtained suspension was dialyzed, and the dialyzed suspension was washed by centrifugation several times to obtain based graphene.

[0039] B. Add 10~100mg Os(diamino-bpy) 2 ClIm(CH 2 ) 11 -NH 2 Fully dissolve in ethanol under stirring, add 100 μL of acryloyloxy-modified graphene dispersion solution with a concentration of 200 mg / mL, and stir at 40-90° C. for 1-12 hours. After the reaction is completed, the obtained product is washed by centrifugation with ethanol, and then dia...

Embodiment 3

[0042] This embodiment provides a redox ligand-based conductive nanomaterial glucose sensing material, the preparation process of which includes the following steps:

[0043] A. Weigh 1-100mg of nano-conductive carbon black and place it in a 10mL vial, add 1-3mL of deionized water and 0.01-1mL of γ-glycidyl etheroxypropyl trimethoxysilane, and sonicate for 10-100min to obtain a uniform The dispersion liquid was placed at 25-50°C for 1-24 hours, 0.1-100 μL of 0.1M hydrochloric acid was added to it, and it was left at room temperature for 6-48 hours. The obtained suspension was dialyzed, and the dialyzed suspension was washed by centrifugation several times to obtain a ring on the surface. Oxygen nano conductive carbon black.

[0044] B. Add 10~100mg Os(dimethyl-bpy) 2 ClIm(CH 2 ) 11 -NH 2 Fully dissolve in ethanol under stirring, add 100 μL of epoxy-modified conductive carbon black dispersion with a concentration of 200 mg / mL, and stir at 40-90° C. for 1-12 hours. After th...

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Abstract

The invention discloses a conductive nano-material glucose sensing material as well as a preparation method and application thereof, and relates to the technical field of special materials for intelligent glucose monitoring. The conductive nano-material glucose sensing material provided by the invention is obtained by covalent cross-linking of a conductive nano-material containing a redox ligand and glucose oxidase through a cross-linking agent. According to the present invention, the electron transfer rate of the medium is increased through the conductive nanometer material with the redox ligand, such that the oxygen effect in the current wired enzyme technology is overcome, and the electrochemical glucose sensor prepared from the material can specifically detect glucose, the linear correlation coefficient of the stable current signal and the glucose concentration is high, and the influence of acetaminophen serving as an interfering substance on glucose detection can be eliminated. Therefore, the glucose sensing material based on the conductive nano material modified by the redox ligand has wide application value in intelligent glucose monitoring.

Description

technical field [0001] The invention relates to the technical field of special materials for glucose intelligent monitoring and its application, in particular to a conductive nanomaterial glucose sensing material and its preparation method and application. Background technique [0002] Currently commercialized continuous blood glucose monitors (CGMS) use electrochemical methods based on enzymatic reactions to convert glucose concentration into electrical signals in real time. These CGMS products all contain glucose oxidase, which can specifically recognize glucose and is insensitive to other sugars in the organism. The technologies used by the commercially available CGMS can be divided into first-generation enzyme technology and second-generation wired enzyme technology. [0003] The first-generation enzyme technology indirectly detects glucose concentration by detecting the concentration of hydrogen peroxide produced during glucose oxidation, and the working electrode used...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3277G01N27/3278
Inventor 王云兵胡雪丰张婕妤鲁玉辉
Owner 江苏跃凯生物技术有限公司
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