Analysis device and method for atrial fibrillation myocardial fibrosis

Abstract

The invention relates to an atrial fibrillation myocardial fibrosis analysis device and method. The device comprises a space transcription module, an expression change differential gene screening module, a dimension reduction processing module, a clustering analysis module, a clustering differential gene set screening module, a main difference class group screening module and an analysis module. A space transcriptome technology is adopted to carry out space transcriptome sequencing on animal myocardial tissue slices of a normal group and a model group, space transcriptome sequencing data is utilized to carry out subsequent analysis, screening of highly variable genes, dimension reduction and clustering processing, differential genes of each cluster are screened, the screened differential genes of each cluster are subjected to enrichment analysis, and the content of the cardiac muscle tissue slices of the normal group and the model group is obtained. And finally, carrying out enrichment analysis on the differential genes of the main differential cluster. The device disclosed by the invention can be used for analyzing key characteristic genes and related molecular signal channels in an atrial fibrillation atrial muscle fibrillation lesion mechanism, so that an effective molecular theoretical basis and theoretical support are provided for better knowing the occurrence and development process of atrial fibrillation lesion.

Description

technical field [0001] The invention belongs to the field of internal medicine of traditional Chinese medicine and biological information technology, and relates to a method for analyzing the pathological mechanism of atrial fibrillation and atrial muscle fibrosis by using spatial transcriptome technology. Background technique [0002] Complex heterogeneous tissues are composed of diverse cell types and spaces. The study of these complex heterogeneous tissues requires tools capable of assessing gene expression profiles and the spatial organization of multiple cell types and states. Formal analysis of tissues at the cellular and molecular levels provides biological information while preserving the organization of the tissue and cellular microenvironment. [0003] There is no tissue dissociation bias in transcriptome analysis because the tissue is intact and allows determination of cell types in heterogeneous tissues. The Visium Spatial Gene Expression Solution, from 10X, pr...

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Problems solved by technology

[0011] The purpose of the present invention is to solve the problem that the research method of complex heterogeneous tissue in myocardial tissue caused by atrial fibrillation is complicated at the cellular and molecular levels, and the spatial position information of cells, genes and mutant genes in the lesion is not clear, and provides a method using The method of spatial transcriptome technology to analyze the pathological mechanism of atrial fibrosis in atrial fibrillation solves the problem that existing research methods cannot clearly analyze and determine the mechanism of the occurrence and development of atrial fibrosis in atrial fibrillation

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