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Silk protein gel-based rotator cuff patch and preparation method thereof

A technology of silk protein and silk protein solution, which is applied in the field of rotator cuff patch and patch preparation, and can solve problems such as local inflammation and foreign body reaction

Active Publication Date: 2022-04-26
福州市第二医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Artificial grafts have advantages in terms of biomechanics, but there are problems such as foreign body reactions or local inflammation

Method used

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  • Silk protein gel-based rotator cuff patch and preparation method thereof
  • Silk protein gel-based rotator cuff patch and preparation method thereof
  • Silk protein gel-based rotator cuff patch and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation of embodiment 1 patch

[0041]The regenerated silk protein solution is made using the following steps:

[0042] Boil the cocoons in boiling water with a sodium bicarbonate concentration of 0.5wt% for 30 minutes, wash them with water three times, then boil them for another 30 minutes under the same conditions, take them out, wash them with warm water three times, and then wash them with deionized water for four times. Oven drying at 40°C for 24 hours to obtain degummed silk;

[0043] Put the degummed silk in a lithium bromide aqueous solution with a concentration of 9.3mol / L, heat it in a water bath at 60°C for 1 hour, filter the dissolved regenerated silk protein solution with eight layers of gauze to remove insoluble impurities, and then pour the filtered solution into a dialysis bag and dialyzed in deionized water for three days;

[0044] The dialyzed solution was centrifuged at a speed of 8000 rpm, centrifuged for 10 minutes at a temperature of 4°C-...

Embodiment 2

[0050] Embodiment 2 performance test

[0051] Animal experiment groups: a. silk protein spinning group; b. silk protein spinning silk protein gel group.

[0052] Weaving of the patch: The two ends of the patch are woven with sutures so that it can be used to suture the rotator cuff stump and fix the humeral head. The size of the patch after weaving is 10mm in length, 4mm in width, and 1mm in thickness, which is suitable for the rabbit infraspinatus tendon suture model. The sutured patch was subjected to biomechanical tests, which met the maximum demand of about 130N for rabbit infraspinatus muscle tear.

[0053] ① 2.5-3.5kg New Zealand white rabbits were anesthetized and skin-prepared for the shoulder joint. A parallel incision was made about 4 cm above the infraspinatus tendon, and blunt separation was performed layer by layer to expose the infraspinatus tendon. The infraspinatus muscle was resected close to the insertion point of the humerus, and the stump and bone surface...

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PUM

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Abstract

A rotator cuff patch based on fibroin gel comprises a rotator cuff patch body, a fibroin electrostatic spinning membrane serving as a patch base layer, fibroin gel containing Mg < 2 + > ions serving as a load anti-inflammatory drug layer and fibroin gel containing Zn < 2 + > ions serving as a load healing promoting drug layer, and the rotator cuff patch has the full-degradation characteristic, inhibits inflammatory response in the early stage, promotes osteogenesis in the repair period, and has the advantages of being free of side effects, free of side effects and the like. The effect of promoting graft integration is achieved, and the biological safety is higher.

Description

technical field [0001] The invention belongs to the field of tissue repair, and in particular relates to a rotator cuff patch based on silk protein gel coating, which is used for the repair of the rotator cuff, inhibits the occurrence and development of inflammation, and is beneficial to further play the role of promoting healing, with higher safety , and a method for making a patch. Background technique [0002] Rotator cuff tears cause joint pain, decreased function, stiffness, and limited range of motion, and impose a substantial economic burden on society. A giant rotator cuff tear refers to a rotator cuff injury with a tear greater than 5 cm or involving ≥ 2 tendons. Currently, surgical repair is the main treatment. Some patients' postoperative shoulder function has been greatly improved, but the re-tear rate is high, up to 15% to 94%. The cause of re-tear is related to factors such as tear size, blood supply, fat infiltration, age, smoking, and muscle atrophy. Studi...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/22A61L27/02A61L27/18A61L27/50A61L27/54A61L27/58B32B9/02B32B9/04B32B27/04A61F2/08C07K14/435C07K1/34C07K1/14D04H1/728
CPCA61L27/227A61L27/025A61L27/18A61L27/50A61L27/54A61L27/58B32B5/02B32B5/26A61F2/08C07K14/43586D04H1/728A61L2300/41A61L2300/412A61L2300/602A61L2300/102A61L2300/402A61L2430/10B32B2260/021B32B2260/046B32B2262/08B32B2535/00A61F2002/0894C08L89/00C08L71/02
Inventor 张韬刘楠楠吴冬枝何文慧
Owner 福州市第二医院
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