Polyethyleneimine derivative and application thereof in preparation of immunologic adjuvant

A polyethyleneimine, immune adjuvant technology, applied in the field of biomedicine, can solve the problems of recurrence and metastasis, incomplete tumor removal, etc., and achieve the effects of promoting maturation, good biological safety, and low cytotoxicity

Active Publication Date: 2022-04-26
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in most cases, the body cannot generate a strong immune response only by inducing ICD, and as a result, the tumor cannot be completely eliminated, resulting in recurrence and metastasis.

Method used

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  • Polyethyleneimine derivative and application thereof in preparation of immunologic adjuvant
  • Polyethyleneimine derivative and application thereof in preparation of immunologic adjuvant
  • Polyethyleneimine derivative and application thereof in preparation of immunologic adjuvant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Add 100 mg of polyethyleneimine with a molecular weight of 1.8k and 100 mg of cinnamaldehyde into a 10 mL vial filled with 2 mL of absolute ethanol to obtain polyethyleneimine ethanol solution and cinnamaldehyde ethanol solution respectively.

[0047] (2) The ethanol solution of cinnamaldehyde obtained in step (1) was slowly dropped into a vial containing polyethyleneimine ethanol solution, and stirred by magnetic force at room temperature for 8 hours to obtain a reaction solution.

[0048] (3) Add the reaction solution obtained in step (2) into a 100 ml single-necked round bottom flask, remove the organic solvent with a rotary evaporator, wash with absolute ethanol twice, and then dry overnight in a vacuum oven to obtain a light yellow solid That is, cinnamaldehyde-modified polyethyleneimine derivatives.

[0049] (4) Dissolve the cinnamaldehyde-modified polyethyleneimine derivative obtained in step (3) in 2 mL of absolute ethanol to obtain a cinnamaldehyde-modified p...

Embodiment 2

[0062] (1) Add 100 mg of polyethyleneimine with a molecular weight of 1.8k and 100 mg of 3-phenylpropanal into a 10 mL vial filled with 2 mL of absolute ethanol to obtain polyethyleneimine ethanol solution and 3- Phenylpropanal ethanol solution.

[0063] (2) Slowly drop the 3-phenylpropionaldehyde ethanol solution obtained in step (1) into a vial containing polyethyleneimine ethanol solution, and magnetically stir for 6 hours at room temperature to obtain a reaction solution.

[0064] (3) Add the reaction liquid obtained in step (2) into a 100 ml single-necked round-bottomed flask, remove the organic solvent with a rotary evaporator, wash with absolute ethanol twice, and then dry overnight in a vacuum oven to obtain a white solid that is 3-Phenylpropanal modified polyethyleneimine derivatives.

[0065] (4) Dissolve the 3-phenylpropanal-modified polyethyleneimine derivative obtained in step (3) in 2 mL of absolute ethanol to obtain a 3-phenylpropanal-modified polyethyleneimine...

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Abstract

The invention discloses a polyethyleneimine derivative and application thereof in preparation of an immunologic adjuvant, and belongs to the technical field of biological medicine. According to the invention, aryl aldehyde is modified on polyethyleneimine through Schiff alkali reaction to prepare a polyethyleneimine derivative, and the polyethyleneimine derivative is further prepared into the functional nano adjuvant. The nano adjuvant can effectively promote activation of cytotoxic T cells by promoting maturation of DC cells and phagocytosis of antigens; after being combined with a chemical drug, the compound can enhance the immune efficacy of the chemical drug and relieve the systemic toxicity of the chemical drug, so that tumor killing is effectively realized.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a polyethyleneimine derivative and its application in preparing immune adjuvants. Background technique [0002] With the success of immune checkpoint blockade (Immune Checkpoint Blockade, ICB) and chimeric antigen receptor T cell therapy (Chimeric Antigen Receptor T cell, CAR-T) in clinical trials, tumor immunotherapy, an emerging cancer treatment The method then became a research hotspot. Tumor immunotherapy is a treatment method that inhibits tumor growth and eliminates tumors by activating the body's own immune system. At present, most of the patients with tumor immunotherapy are patients with relatively certain tumor antigen epitopes. However, for patients with high tumor mutation rates and difficult to obtain antigens, immunotherapy still has certain difficulties. In the immunotherapy method, the systemic immune response is usually activated by treating the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G73/02A61K31/704A61K39/39A61P37/04
CPCC08G73/0206A61K39/39A61K31/704A61P37/04A61K2039/55511A61K2300/00Y02P20/55
Inventor 孙敏捷杨若熙周占威
Owner CHINA PHARM UNIV
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