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High performance liquid detection method for diester alkaloids in aconitum sinomontanum

A diester-type alkaloid, high-performance liquid phase technology, applied in the field of medicine

Active Publication Date: 2022-04-26
斯琴巴特尔
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no quantitative detection method for diester-type aconitine in Gabu Ridilu mixture

Method used

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  • High performance liquid detection method for diester alkaloids in aconitum sinomontanum
  • High performance liquid detection method for diester alkaloids in aconitum sinomontanum
  • High performance liquid detection method for diester alkaloids in aconitum sinomontanum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Detection of Diester Type Alkaloids in Aconitum Aconitum

[0033] 1.1 Preparation of the test solution

[0034] Accurately measure 10mL of this product, evaporate to dryness, accurately add 10mL of absolute ethanol to the residue, weigh it, ultrasonically treat it (power 300W, frequency 40kHz) for 30 minutes, let it cool, weigh again, and make up the lost amount with absolute ethanol. Weight, shake well, filter, take the continued filtrate, that is.

[0035] 1.2 Preparation of reference solution

[0036] Take an appropriate amount of aconitine reference substance, hypoaconitine reference substance and neoaconitine reference substance, weigh them accurately, add isopropanol-chloroform (1:1) mixed solution to make aconitine concentration of 31.7 μg / mL, a solution with a concentration of hypoaconitine of 15.5 μg / mL and a concentration of neoaconitine of 41.4 μg / mL.

[0037] 1.3 Chromatographic conditions

[0038] Select Supfex AQ-C18 column (4.6×250mm, 5μm) a...

Embodiment 2

[0040] The difference between Example 2 and Example 1 lies in the chromatographic conditions: acetonitrile-tetrahydrofuran (20:10)-0.1mol / L ammonium acetate solution (0.5mL of glacial acetic acid per 1000mL) (30:70) is the mobile phase; the detection wavelength It is 230nm, the column temperature is 28°C, and the flow rate is 0.9mL / min.

Embodiment 3

[0042] The difference between Example 3 and Example 1 lies in the chromatographic conditions: acetonitrile-tetrahydrofuran (30:20)-0.1mol / L ammonium acetate solution (0.5mL of glacial acetic acid per 1000mL) (30:70) is used as the mobile phase; the detection wavelength It is 240nm, the column temperature is 32°C, and the flow rate is 1.1mL / min.

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Abstract

The invention belongs to the technical field of medicines, and particularly relates to a high performance liquid detection method for diester alkaloids in aconitum sinomontanum. According to the high performance liquid detection method for the diester alkaloids in the aconitum sinomontanum, octadecyl silane bonded silica gel is used as a filling agent; the mobile phase A is acetonitrile-tetrahydrofuran, and the mobile phase B is a 0.1 mol / L ammonium acetate solution; the mobile phase A and the mobile phase B are subjected to isocratic elution according to the volume ratio of 30: 70. According to the present invention, with the chromatographic conditions, the diester type aconitine in the Garidiluu mixture can be effectively detected, the aconitine detection limit is 0.8397 [mu] g / mL, the hypaconitine detection limit is 2.1528 [mu] g / mL, and the mesaconitine detection limit is 2.1904 [mu] g / mL. The quantitation limit of aconitine is 4.1987 [mu] g / mL, the quantitation limit of hypaconitine is 10.7639 [mu] g / mL, and the quantitation limit of mesaconitine is 10.9524 [mu] g / mL.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a high-efficiency liquid-phase detection method for diester-type alkaloids in Aconitum aconitum. Background technique [0002] Aconitum excelsum (scientific name: Aconitum excelsum Reichb.), a plant of the genus Aconitum in the family Ranunculaceae, a perennial herb. Its roots have the effects of promoting blood circulation, relieving pain and reducing swelling. Zihuagao aconitum is distributed in northwestern Liaoning (Keshiketeng Banner) and western Heilongjiang in China. It is also distributed in Mongolia, Siberia and European parts of the Soviet Union. [0003] Gabu Ridilu Mixture is a self-made reagent of Xilin Gol Mengmeng Medical Hospital. The main ingredients are Zihuagao aconitum and sucrose. This product is a brown liquid; a little sediment is normal; it tastes sweet, slightly sour, and bitter. The main effects of Gabu Ridilu Mixture are clearing lung he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8675G01N2030/047
Inventor 斯琴巴特尔席琳图雅李旻辉青格勒
Owner 斯琴巴特尔