Preparation method of bee pollen enzyme
A bee pollen and enzyme technology, applied in the field of microorganisms or enzymes or their compositions, can solve the problems that the content cannot be directly used by the human body, allergic reactions, lack of elasticity, etc.
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Embodiment 1
[0037] A kind of preparation method of bee pollen enzyme of the present invention, comprises the steps:
[0038] a Preparation of seed liquid: Inoculate Lactobacillus plantarum 322Hp (Lactobacillus plantarum 322Hp), Lactobacillus acidophilus 322Ha (Lactobacillus acidophilus 322Ha) and Lactobacillus reuteri 322Hr (Lactobacillus reuteri 322Hr) into MRS respectively Shake culture in the medium at 37°C and 180r / min in a shaker for 28-36h until Lactobacillus plantarum 322Hp, Lactobacillus acidophilus 322Ha and Lactobacillus reuteri 322Hr (Lactobacillus OD of reuteri 322Hr) 600 Reached around 2.0, 2.1 and 1.5. The MRS medium is peptone 10g / L, beef extract 5g / L, yeast extract 4g / L, glucose 20g / L, dipotassium hydrogen phosphate 2g / L, sodium acetate trihydrate 5g / L, diammonium hydrogen citrate 2g / L, magnesium sulfate heptahydrate 0.2g / L, manganese sulfate monohydrate 0.005g / L, the pH of the MRS medium is 6.5;
[0039] b Preparation of enzyme fermentation medium: add pasteurized hon...
Embodiment 2
[0043] A kind of preparation method of bee pollen enzyme of the present invention, comprises the steps:
[0044] a Preparation of seed liquid: Inoculate Lactobacillus plantarum 322Hp (Lactobacillus plantarum 322Hp), Lactobacillus acidophilus 322Ha (Lactobacillus acidophilus 322Ha) and Lactobacillus reuteri 322Hr (Lactobacillus reuteri 322Hr) into MRS respectively Shake culture in the medium at 37°C and 180r / min in a shaker for 28-36h until Lactobacillus plantarum 322Hp, Lactobacillus acidophilus 322Ha and Lactobacillus reuteri 322Hr (Lactobacillus OD of reuteri 322Hr) 600 Reached around 2.0, 2.1 and 1.5. The MRS medium is peptone 10g / L, beef extract 5g / L, yeast extract 4g / L, glucose 20g / L, dipotassium hydrogen phosphate 2g / L, sodium acetate trihydrate 5g / L, diammonium hydrogen citrate 2g / L, magnesium sulfate heptahydrate 0.2g / L, manganese sulfate monohydrate 0.005g / L, the pH of the MRS medium is 6.5;
[0045] b Preparation of enzyme fermentation medium: add pasteurized hon...
Embodiment 3
[0049] A kind of preparation method of bee pollen enzyme of the present invention, comprises the steps:
[0050] a Preparation of seed liquid: Inoculate Lactobacillus plantarum 322Hp (Lactobacillus plantarum 322Hp), Lactobacillus acidophilus 322Ha (Lactobacillus acidophilus 322Ha) and Lactobacillus reuteri 322Hr (Lactobacillus reuteri 322Hr) into MRS respectively Shake culture in the medium at 37°C and 180r / min for 28-36h until Lactobacillus plantarum 322Hp, Lactobacillus acidophilus 322Ha and Lactobacillus reuteri 322Hr (Lactobacillus plantarum 322Hp) OD of reuteri 322Hr) 600 Reached around 2.0, 2.1 and 1.5. The MRS medium is peptone 10g / L, beef extract 5g / L, yeast extract 4g / L, glucose 20g / L, dipotassium hydrogen phosphate 2g / L, sodium acetate trihydrate 5g / L, diammonium hydrogen citrate 2g / L, magnesium sulfate heptahydrate 0.2g / L, manganese sulfate monohydrate 0.005g / L, the pH of the MRS medium is 6.5;
[0051] B prepares ferment fermentation medium: add rape pollen aft...
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