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Preparation method of bee pollen enzyme

A bee pollen and enzyme technology, applied in the field of microorganisms or enzymes or their compositions, can solve the problems that the content cannot be directly used by the human body, allergic reactions, lack of elasticity, etc.

Pending Publication Date: 2022-05-06
株式会社APLCARE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, because the outer wall of bee pollen is thicker, harder, and less elastic, its main component is sporopollenin, which has strong water resistance, permeability resistance, corrosion resistance, acid and alkali resistance, and biodegradability. Most of the chemical substances do not react, so that the rich content cannot be directly used by the human body, and some of the proteins in it are prone to allergic reactions in some people, so it is necessary to release the nutrients inside the pollen by breaking the wall

Method used

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  • Preparation method of bee pollen enzyme
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  • Preparation method of bee pollen enzyme

Examples

Experimental program
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Effect test

Embodiment 1

[0037] A kind of preparation method of bee pollen enzyme of the present invention, comprises the steps:

[0038] a Preparation of seed liquid: Inoculate Lactobacillus plantarum 322Hp (Lactobacillus plantarum 322Hp), Lactobacillus acidophilus 322Ha (Lactobacillus acidophilus 322Ha) and Lactobacillus reuteri 322Hr (Lactobacillus reuteri 322Hr) into MRS respectively Shake culture in the medium at 37°C and 180r / min in a shaker for 28-36h until Lactobacillus plantarum 322Hp, Lactobacillus acidophilus 322Ha and Lactobacillus reuteri 322Hr (Lactobacillus OD of reuteri 322Hr) 600 Reached around 2.0, 2.1 and 1.5. The MRS medium is peptone 10g / L, beef extract 5g / L, yeast extract 4g / L, glucose 20g / L, dipotassium hydrogen phosphate 2g / L, sodium acetate trihydrate 5g / L, diammonium hydrogen citrate 2g / L, magnesium sulfate heptahydrate 0.2g / L, manganese sulfate monohydrate 0.005g / L, the pH of the MRS medium is 6.5;

[0039] b Preparation of enzyme fermentation medium: add pasteurized hon...

Embodiment 2

[0043] A kind of preparation method of bee pollen enzyme of the present invention, comprises the steps:

[0044] a Preparation of seed liquid: Inoculate Lactobacillus plantarum 322Hp (Lactobacillus plantarum 322Hp), Lactobacillus acidophilus 322Ha (Lactobacillus acidophilus 322Ha) and Lactobacillus reuteri 322Hr (Lactobacillus reuteri 322Hr) into MRS respectively Shake culture in the medium at 37°C and 180r / min in a shaker for 28-36h until Lactobacillus plantarum 322Hp, Lactobacillus acidophilus 322Ha and Lactobacillus reuteri 322Hr (Lactobacillus OD of reuteri 322Hr) 600 Reached around 2.0, 2.1 and 1.5. The MRS medium is peptone 10g / L, beef extract 5g / L, yeast extract 4g / L, glucose 20g / L, dipotassium hydrogen phosphate 2g / L, sodium acetate trihydrate 5g / L, diammonium hydrogen citrate 2g / L, magnesium sulfate heptahydrate 0.2g / L, manganese sulfate monohydrate 0.005g / L, the pH of the MRS medium is 6.5;

[0045] b Preparation of enzyme fermentation medium: add pasteurized hon...

Embodiment 3

[0049] A kind of preparation method of bee pollen enzyme of the present invention, comprises the steps:

[0050] a Preparation of seed liquid: Inoculate Lactobacillus plantarum 322Hp (Lactobacillus plantarum 322Hp), Lactobacillus acidophilus 322Ha (Lactobacillus acidophilus 322Ha) and Lactobacillus reuteri 322Hr (Lactobacillus reuteri 322Hr) into MRS respectively Shake culture in the medium at 37°C and 180r / min for 28-36h until Lactobacillus plantarum 322Hp, Lactobacillus acidophilus 322Ha and Lactobacillus reuteri 322Hr (Lactobacillus plantarum 322Hp) OD of reuteri 322Hr) 600 Reached around 2.0, 2.1 and 1.5. The MRS medium is peptone 10g / L, beef extract 5g / L, yeast extract 4g / L, glucose 20g / L, dipotassium hydrogen phosphate 2g / L, sodium acetate trihydrate 5g / L, diammonium hydrogen citrate 2g / L, magnesium sulfate heptahydrate 0.2g / L, manganese sulfate monohydrate 0.005g / L, the pH of the MRS medium is 6.5;

[0051] B prepares ferment fermentation medium: add rape pollen aft...

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Abstract

The invention discloses a preparation method suitable for bee pollen enzyme. The preparation method comprises the following steps: a, preparing a seed solution, namely activating lactobacillus plantarum 322Hp, lactobacillus acidophilus 322Ha and lactobacillus reuteri 322Hr; b, preparing an enzyme fermentation culture medium: adding pasteurized honey into sterile water, then adding bee pollen subjected to mechanical wall breaking, and uniformly mixing; c, preparing enzyme fermentation liquid: inoculating the seed liquid into a bee pollen enzyme fermentation culture medium, and performing shaking culture and fermentation through a shaking table to obtain a fermentation product; d, preparing an enzyme finished product; according to the method, the bee pollen is treated by adopting a method of combining mechanical wall breaking and mixed fermentation of three kinds of lactic acid bacteria, so that the wall breaking rate of the pollen is increased, pollen contents are more sufficiently released, the nutritional value and active ingredients of the bee pollen are effectively increased, and meanwhile, main allergens in the pollen can be removed; the market audiences of the bee pollen enzyme are greatly increased.

Description

technical field [0001] The present invention relates to the technical field of microorganisms or enzymes or their compositions, in particular to a fermentation method suitable for bee pollen enzymes. Background technique [0002] Bee pollen refers to the pollen grains collected by bees from the male anthers of angiosperms and the microsporangia on the microspore leaves of gymnosperms, and processed by bees. Bee pollen is a natural natural resource, rich in nutrients, it not only contains essential sugars, proteins, free amino acids, nucleic acids, unsaturated fatty acids, vitamins, minerals, trace elements and phenols and other activities Composition, and nutrients are comprehensive and balanced, known as "miniature nutrition bank". Bee pollen is not only a good natural nutritious food, but also has anti-oxidation, anti-bacterial and anti-inflammatory, anti-tumor, anti-allergic and immune-enhancing effects, and has always been favored by consumers. [0003] Common bee poll...

Claims

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Application Information

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IPC IPC(8): A23L33/00A23L5/20
CPCA23L33/00A23L5/28A23V2002/00A23V2400/113A23V2400/173A23V2400/169A23V2200/30A23V2200/302A23V2200/304A23V2200/324A23V2200/322A23V2200/318
Inventor 张庆华程网山
Owner 株式会社APLCARE
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