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Method for increasing oil content of flue gas culture oil-producing microalgae

A technology for oil-producing microalgae and oil content, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of microalgae carbon fixation inhibition, low carbon fixation efficiency, etc. The effect of increasing oil content

Active Publication Date: 2022-05-06
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the flue gas contains harmful substances such as SOx and NOx at the same time, which can inhibit the carbon fixation and growth of microalgae
Researchers have found in practical applications that when CO in the environment 2 When the volume fraction is greater than 5v%, the growth of most microalgae will be inhibited, and the carbon fixation efficiency will be low; 2 The concentration is generally 10%-20%, and it also contains substances that are toxic to microalgae, such as SOx, NOx, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] In the 20L photobioreactor, add the seed liquid of algae HCY-BY1 and the microalgae medium, the amount of the seed liquid added is 800mL, the amount of the microalgae medium is 8L, and the flue gas is passed into the light and dark alternate culture. The concentration of SOx in flue gas is 0.03v%, NOx0.05v%, CO 2 The concentration is 14v%. The light-dark cycle is 24 hours, the light-dark time ratio is 14:10, the light intensity is 5000Lux, the culture temperature is 20°C, and the pH value is 6-9.

[0031] After 72 hours of alternating light and dark culture, the denitrifying bacteria seed liquid was inoculated, and the volume ratio of the microalgae culture system was 1:40, and the culture was terminated after 168 hours of culture.

[0032] The microalgae cells were harvested by centrifugation, and the dry cell weight and oil content were determined. After vacuum freeze-drying at -60°C to constant weight, the dry weight of the algae powder was measured, the biomass pr...

Embodiment 2

[0034]In the 20L photobioreactor, add the seed liquid of algae HCY-BY1 and the microalgae medium, the amount of the seed liquid is 400mL, the amount of the microalgae medium is 8L, and the flue gas is passed through to carry out alternating light and dark culture. The concentration of SOx in flue gas is 0.02v%, NOx0.06v%, CO 2 The concentration is 13.5v%. The light-dark cycle is 24 hours, the light-dark time ratio is 14:10, the light intensity is 5000 Lux, the culture temperature is 25°C, and the pH value is 6-9.

[0035] After 72 hours of alternating light and dark culture, the denitrifying bacteria seed solution was inoculated with a volume ratio of 1:30 to the microalgae culture system, and the culture was terminated after 180 hours of culture.

[0036] The microalgae cells were harvested by centrifugation, and the dry cell weight and oil content were determined. After vacuum freeze-drying at -60°C to constant weight, the dry weight of the algae powder was measured, the b...

Embodiment 3

[0038] In the 20L photobioreactor, add the seed liquid of algae HCY-BY1 and the microalgae medium, the amount of the seed liquid added is 800mL, the amount of the microalgae medium is 8L, and the flue gas is passed into the light and dark alternate culture. The concentration of SOx in flue gas is 0.04v%, NOx0.03v%, CO 2 The concentration is 13v%. The light-dark cycle is 24 hours, the light-dark time ratio is 14:10, the light intensity is 8000 Lux, the culture temperature is 30°C, and the pH value is 6-9.

[0039] After 48 hours of alternating light and dark culture, the denitrifying bacteria seed liquid was inoculated, and the volume ratio of the microalgae culture system was 1:50, and the culture was terminated after 168 hours of culture.

[0040] The microalgae cells were harvested by centrifugation, and the dry cell weight and oil content were determined. After vacuum freeze-drying at -60°C to constant weight, the dry weight of the algae powder was measured, the biomass p...

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PUM

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Abstract

The invention relates to a method for increasing the oil content of flue gas culture oil-producing microalgae, which comprises the following steps: (1) flue gas is introduced into a photobioreactor for microalgae culture, the microalgae is strepton HSC-BY1 (Desmodesmus abundans), and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.19982; and (2) culturing until the early stage of the stable phase, inoculating denitrifying bacteria, and continuously culturing until the later stage of the stable phase. According to the method, microalgae are cultured by using flue gas, denitrifying bacteria are inoculated when the microalgae are cultured to the earlier stage of a stable phase, and the grease content of the oil-producing microalgae is increased through the synergistic effect of the two microorganisms.

Description

technical field [0001] The invention belongs to the technical field of biomass energy, and in particular relates to a method for increasing the oil content of flue gas cultured oil-producing microalgae. Background technique [0002] Due to the high concentration of carbon dioxide in the flue gas, it can be used for microalgae cultivation. On the one hand, carbon fixation by microalgae is used to achieve CO 2 Emission reduction, and microalgae that produce oil, starch, etc. can be obtained. However, most of the flue gas also contains harmful substances such as SOx and NOx, which can inhibit the carbon fixation and growth of microalgae. Researchers have found in practical applications that when CO in the environment 2 When the volume fraction is greater than 5v%, the growth of most microalgae will be inhibited, and the carbon fixation efficiency will be low; 2 The concentration is generally 10%-20%, and it also contains substances that are toxic to microalgae, such as SOx,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/20C12R1/89C12R1/01
CPCC12N1/12C12N1/20Y02A50/20
Inventor 师文静赵磊王新
Owner CHINA PETROLEUM & CHEM CORP
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