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Uric acid detection kit and preparation method thereof

A technology of kits and reagents, which is applied in measurement devices, measurement of color/spectral properties, and material analysis by observing the impact on chemical indicators, can solve the problems of complex reagents and difficulty in ensuring accuracy, and achieve a simple method. , high accuracy and good stability

Pending Publication Date: 2022-05-06
SUZHOU BAIYUAN GENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing kits generally use complex reagents, and the accuracy is difficult to guarantee

Method used

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  • Uric acid detection kit and preparation method thereof
  • Uric acid detection kit and preparation method thereof
  • Uric acid detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] This embodiment provides a preparation method of the kit, and the specific steps are as follows:

[0031] (1) Preparation of 1mol / L disodium hydrogen phosphate solution: take 358.14g of analytically pure disodium hydrogen phosphate dodecahydrate, add purified water to 1000mL, and 2 High pressure for 30 minutes, then dilute to 1000mL;

[0032] Preparation of 1mol / L sodium dihydrogen phosphate solution: take 156.01g of analytically pure sodium dihydrogen phosphate dihydrate, add purified water to 1000mL, 2 High pressure for 30 minutes, then dilute to 1000mL;

[0033] Mix 845 mL of disodium hydrogen phosphate solution and 155 mL of sodium dihydrogen phosphate solution, dilute to 10000 mL with purified water, and obtain a phosphate buffer solution with a pH of 7.6 and a concentration of 100 mmol / L;

[0034] (2) Take 5000U peroxidase and 296mg N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt, add it to 1L phosphate buffer, mix well to get first reagent;

...

Embodiment 2

[0039] This embodiment is the application of the kit obtained in Example 1, and measures 3 clinical urine samples. The specific steps are as follows:

[0040] (1) prepare the uric acid solution of a series of standard samples, its uric acid concentration is successively 10mg / L, 20mg / L, 30mg / L, 40mg / L, 50mg / L, 100mg / L, 150mg / L, 250mg / L;

[0041] (2) Take 80 μL of uric acid solution of a series of standard samples and add 2 mL of diluent to dilute, then add 400 μL of the first reagent and 20 μL of the second reagent to fully react, use a spectrophotometer to read the absorbance value at 540 nm, and the results are shown in Table 1 Shown:

[0042] The absorbance of the uric acid solution of a series of standard samples of table 1

[0043]

[0044] Standard curve y=0.0021x+0.0024 can be obtained from Table 1, R 2 =0.999, where y is the absorbance, and x is the concentration of uric acid;

[0045] (3) Take 80 μL of clinical urine sample and add 2 mL of diluent to dilute, then...

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Abstract

The uric acid detection kit comprises a first reagent, a second reagent and a diluent, the first reagent comprises a phosphate buffer and polyethylene glycol 6000, and the second reagent further comprises polyoxyethylene lauryl ether, sodium azide and a goat anti-human uric acid antibody. The invention also discloses preparation of the kit. According to the kit disclosed by the invention, uric acid in urine is oxidized under the action of uricase to generate allantoin and hydrogen peroxide, then the hydrogen peroxide, 4-aminoantipyrine (4-AAP) and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt (TOOS) are catalyzed by peroxidase to generate a colored substance, and the colored substance is used as a detection reagent. The concentration of uric acid in a human body can be quantitatively detected, the method is simple, and the used reagents are common reagents, so that daily inspection of uric acid is facilitated; meanwhile, the kit is high in accuracy and good in stability when used for detecting uric acid and is suitable for large-scale use.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a uric acid detection kit and a preparation method thereof. Background technique [0002] Uric acid is the end product of purine metabolism in nucleic acid (including nucleic acid in food) in the body. The liver is the main place where uric acid is produced. When nephropathy affects excretory function, the content of serum uric acid increases. Diseases with excessive nucleic acid catabolism in the body, such as chronic leukemia, multiple myeloma, and polycythemia vera, can also increase uric acid in the blood, but the increase in uric acid is mainly seen in gout, so the content of uric acid is currently the main indicator for diagnosing gout. project. [0003] Gout is a common disease caused by the deposition of urate crystals, which can affect the feet, most commonly the first metatarsophalangeal joint, causing acute inflammatory reactive synovitis. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/78G01N21/31G01N2021/775
Inventor 杨立蒋刈杨立坤车团结常运朝沈荣
Owner SUZHOU BAIYUAN GENT CO LTD
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