Preparation method of composite antihypertensive polypeptide edible packaging film
A packaging film and blood pressure-lowering technology, which is applied in the field of preparation of edible packaging film of compound hypotensive polypeptide, can solve the problems of long-term use, easy to produce drug resistance, increase the burden of human liver metabolism, etc., and achieve convenient and low-cost preparation methods , good tensile strength and elongation at break
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Embodiment 1
[0024] A preparation method of a composite antihypertensive polypeptide edible packaging film, comprising the following steps:
[0025] (1) Grind the soybean grains with a pulverizer, then pass through a 60-mesh sieve, then dissolve the obtained soybean powder into deionized water and adjust the solid-liquid ratio to 6mg / mL, and adjust the temperature to 50°C under the condition of a water bath , adjust the pH value to 8.5, then use Alcalase 2.4L alkaline protease, enzymatically hydrolyze for 3h (enzyme amount 1.85mL / (100g)), then heat at 100°C for 10min to inactivate the enzyme, and put the material at a speed of 8000rmp Centrifuge for 20 minutes, collect the hydrolyzate, and then freeze-dry to obtain the freeze-dried powder of the bioenzyme hydrolyzate;
[0026] (2) Dissolve the freeze-dried hydrolyzate powder obtained in step (1) in deionized water (mass concentration: 40g / L), and heat and stir at 50°C for 20min to reach the optimum temperature of alkaline protease (Alcalas...
Embodiment 2
[0030] The difference between this embodiment and Example 1 is that step (3) is to dissolve 4g soybean polypeptide powder, 4g ovalbumin polypeptide, 1g fructooligosaccharide, 2g chitosan and 0.5g soybean saponin in 110mL of pH5 In deionized water, stir evenly to obtain a mixed solution, and the rest of the operating steps are exactly the same as those in Example 1.
[0031] The content of polypeptide in the packaging film prepared in this embodiment is 40.9%, the content of carbohydrate is 19.8%, the content of soybean saponin is 5.6%, the activity of ACE enzyme is reduced by 70%, and it has better anti-stress effect. Its tensile strength and elongation at break were 40 MPa and 1.6%, respectively.
Embodiment 3
[0033] The difference between this embodiment and Example 1 is that step (3) is to dissolve 4g soybean polypeptide powder, 4g ovalbumin polypeptide, 1g fructooligosaccharide, 2g chitosan and 0.5g soybean saponin in 130mL of 5 pH In deionized water, stir evenly to obtain a mixed solution, and the rest of the operating steps are exactly the same as those in Example 1.
[0034] The content of polypeptide in the packaging film prepared in this embodiment is 32.2%, the content of carbohydrate is 14.2%, the content of soybean saponin is 4.3%, the ACE enzyme activity is reduced by 50%, and it has a certain anti-stress effect. Its tensile strength and elongation at break were 27MPa and 0.6%, respectively.
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Abstract
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