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Microbial strain for high-temperature aerobic composting of biogas residues and application of microbial strain

A technology of aerobic composting and biogas residue, which is applied in the field of environmental microorganisms, can solve the problems of unsatisfactory transformation and maturity of organic matter, ineffective effect of aerobic composting, and inadaptability to environmental conditions, so as to shorten composting time and self-produce Strong heat capacity and good economic benefits

Active Publication Date: 2022-05-10
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Although biogas residue contains a large number of endogenous microorganisms, these microorganisms are mainly anaerobic microorganisms in the process of anaerobic biogas production, which are not suitable for the subsequent environmental conditions of high-temperature aerobic composting, and have little effect on aerobic composting. Relying on the endogenous microorganisms in biogas residues for aerobic composting, the conversion and maturity of organic matter are not ideal

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  • Microbial strain for high-temperature aerobic composting of biogas residues and application of microbial strain
  • Microbial strain for high-temperature aerobic composting of biogas residues and application of microbial strain
  • Microbial strain for high-temperature aerobic composting of biogas residues and application of microbial strain

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preparation example Construction

[0051] The third aspect of the present invention provides a preparation method of the liquid bacterial agent, the preparation method comprising the following steps: inoculating the pure strain of Lithuanian Geobacillus lituanicus G6-1 in a liquid medium for cultivation, and immediately after the cultivation is completed Get a liquid inoculum.

[0052] In one embodiment, after inoculating the pure strain of the Lithuanian Geobacillus lituanicus G6-1 into a liquid medium for cultivation, the culture solution is then inoculated into another liquid medium for expanded cultivation. After the cultivation, the liquid bacterial agent is obtained.

[0053] In one embodiment, the culture temperature is 40-60°C. Specifically, the culture temperature is, for example, 45-55°C, 47-53°C.

[0054] In one embodiment, the stirring speed of the cultivation is 150-200 r / min.

[0055] In one embodiment, the pure strain of Geobacillus lituanicus G6-1 is inoculated into a liquid medium for 13-18 ...

Embodiment 1

[0079] Example 1: Screening and functional verification of biogas residue high-temperature aerobic composting microbial strains

[0080] CYS liquid medium and solid medium are used for the screening and cultivation of strains, and the components are as follows:

[0081] CYS liquid medium: peptone 3g / L, yeast extract 2g / L, soluble starch 1g / L, NaCl 3g / L, MgCl 2 0.125g / L, CaCl 2 0.5g / L, FeSO 4 ·7H 2 O 0.01g / L, trace element solution (Na 2 MoO 4 12g / L, MnCl 2 5g / L, ZnSO 4 ·7H 2 O 0.6g / L, CuSO 4 ·5H 2 O 0.15g / L, CoCl 2 ·6H 2 O 8g / L, NiCl 2 ·6H 2 (00.2g / L) 100μL / L, and the pH value was adjusted to 7-7.5.

[0082] CYS solid medium: add 17g / L agar on the basis of CYS liquid medium.

[0083] After the above mediums were prepared, they were all sterilized by high-pressure steam at 121°C for 20 minutes, and then used for later use.

[0084]Isolation and screening of strains: the strains were isolated by dilution coating method. The experimental samples were collecte...

Embodiment 2

[0089] Example 2: Identification of bacterial strain G6-1

[0090] Genomic DNA of strain G6-1 was extracted and used as a template to amplify strain 16S rDNA using a pair of universal primers (27F, 1492R). The upstream primer is 27F (5'-AGAGTTTGATCCTGGCTCA-3'), and the downstream primer is 1492R (5'-GGTTACCTTGTTACG ACTT-3'). The PCR reaction system (20 μL) is as follows: template DNA 0.5 μL, PCR Taqmix 10 μL, upstream and downstream primers 0.6 μL, add ddH 2 O to 20 μL of the reaction system. PCR program: pre-denaturation at 94°C for 5 min, denaturation at 94°C for 30 s, annealing at 55°C for 30 s, extension at 72°C for 1 min and 30 s, and a total of 30 cycles of the above cycles, extension at 72°C for 10 min, and finally storage at 4°C. PCR products were purified and sequenced by Shanghai Jieli Biotechnology Co., Ltd. The 16SrDNA sequence obtained by sequencing was submitted to NCBI, and the homology sequence comparison analysis was carried out with GenBank by software, an...

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Abstract

The invention relates to the technical field of environmental microorganisms, in particular to a microbial strain for high-temperature aerobic composting of biogas residues and application of the microbial strain, the microbial strain for high-temperature aerobic composting of the biogas residues is Geobacillus variotii G6-1, and the preservation number of the microbial strain is CCTCC NO: M 2022027. The Geobacillus variotii G6-1 is resistant to high temperature and salt, can increase the temperature of a compost body, prolong the high-temperature period of compost, quickly decompose organic matters, accelerate the composting process of biogas residues, shorten the composting time and improve the decomposition degree of materials and the quality of organic fertilizer products, and has high application value.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a microbial strain for high-temperature aerobic composting of biogas residues and an application thereof. Background technique [0002] With the acceleration of urbanization, the output of municipal solid waste is increasing day by day. More than 50% of domestic waste is wet waste, and after waste classification, the output of wet waste increases sharply. As an effective treatment method for wet waste, anaerobic digestion can convert wet waste into renewable energy—methane, which is also in line with the low-carbon development strategy. However, anaerobic digestion will also produce a large amount of solid residues - biogas residues, about 25 kg of biogas residues per ton of wet waste processed. Biogas residue is mainly composed of refractory cellulose and a large number of anaerobic microorganisms. The conventional biogas residue treatment method is to de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/20C12R1/01
CPCC12N1/20C05F17/20Y02W30/40
Inventor 刘莉赵玥吉雨霁史吉平王娜杨宁颜薇芝
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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