Serum-free culture medium for culturing neural stem cells and application of serum-free culture medium

Abstract

The invention discloses a culture medium for culturing neural stem cells and application of the culture medium. The culture medium provided by the invention contains a dryness factor A, a dryness factor B and a dryness factor C, the dryness factor A is selected from at least one of acetate, dichloroacetate and an HMG-CoA reductase inhibitor; the dry factor B is N-acetylcysteine; the dryness factor C is selected from at least one of tryptophan, a tryptophan metabolite and melatonin. The culture medium provided by the invention can improve the dryness of the neural stem cells, avoids differentiation to the greatest extent, achieves the purpose of long-term large-scale culture of the human neural stem cells, and keeps the high multiplication capacity of the neural stem cells at the same time. In addition, the neural stem cell culture medium has clear chemical components, does not contain animal-derived components, reduces the risk of external pathogenic microorganism pollution to the maximum extent, and lays a foundation for clinical large-scale popularization and application in the future.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a serum-free medium for culturing neural stem cells and its application. Background technique [0002] Neurodegenerative diseases result in the continuous degeneration or death of nerve cells. With the aging of the world's population, the prevalence of neurodegenerative diseases represented by dementia is on the rise, but there is currently no effective treatment for such diseases. Recent studies have shown that stem cell therapy is expected to become a new treatment for neurodegenerative diseases, delaying disease progression by differentiating nerve cells or secreting neurotrophic factors. [0003] One property of neural stem cells is self-renewal and pluripotency, ie they can give rise to neurons, astrocytes and oligodendrocytes. Animal experiments have found that transplanted neural stem cells can differentiate into functional neurons in vivo. Therefore, they are of ...

AI Technical Summary

Problems solved by technology

However, the proliferation ability of neural stem cells cultured in Neurobasal medium containing 1% B27 was weak

In addition, in addition to adding more B27 additives to the existing neural stem cell culture medium, it also contains animal extract protein-bovine serum albumin, and there is a risk of introducing animal-derived pathogenic microorganisms

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