Method for determining dosing schemes of doxycycline to Citrobacter freundii of procambarus clarkia in different dosing modes

A technology of citric acid for shrimp and Crayfish, which is applied in the field of drug efficacy of aquatic products, can solve problems such as economic loss, meningitis, enteritis and sepsis, and death of farmed animals, so as to reduce economic loss, improve drug efficiency and improve efficiency. The effect of drug safety

Pending Publication Date: 2022-05-10
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

This bacterium is widely distributed in nature and is an opportunistic pathogenic bacterium that can cause meningitis, enteritis and sepsis in humans and animals, and can also infect shrimp, crab, fish, turtle and other aquaculture animals, leading to the death of farmed animals. cause huge economic losses

Method used

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  • Method for determining dosing schemes of doxycycline to Citrobacter freundii of procambarus clarkia in different dosing modes
  • Method for determining dosing schemes of doxycycline to Citrobacter freundii of procambarus clarkia in different dosing modes
  • Method for determining dosing schemes of doxycycline to Citrobacter freundii of procambarus clarkia in different dosing modes

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Embodiment 1

[0029] 1. Experimental animals

[0030] 300 healthy crayfish (average body weight 19.2±5.5g) were purchased from the experimental base of the Yangtze River Fisheries Research Institute of the Chinese Academy of Fishery Sciences. The test water was continuously aerated with an oxygen pump to keep the dissolved oxygen in the water greater than 4.8mg / L, the pH was 7.2-7.8, and the temperature was divided into two groups at 24±0.5°C. Before the experiment, the crayfish were kept in a glass aquarium for one week.

[0031] 2. Drugs and reagents

[0032] Drugs: Doxycycline powder was purchased from Hubei Aibo Technology Co., Ltd., with a content greater than 98%;

[0033] The standard product doxycycline was purchased from Dr. Ehrenstorfer Company in Germany, and its content was greater than 99%.

[0034] Reagent:

[0035] EDTA-Na2, sodium chloride, magnesium sulfate, sodium dihydrogen phosphate and sodium citrate were purchased from Shanghai Sinopharm Group, analytically pure; C...

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Abstract

The invention discloses a method for determining an administration scheme of doxycycline to citrobacter freundii of procambarus clarkii in different administration modes, and relates to the field of drug effects of aquatic drugs. Comprising the following steps: step 1, separating citrobacter freundii from clinically diseased procambarus clarkia, and measuring the MIC value of doxycycline to the citrobacter freundii by adopting a microdilution method; 2, performing single oral administration on the procambarus clarkii by adopting oral administration, intramuscular injection and venous sinus injection methods respectively, and collecting tissue samples respectively after administration; 3, respectively measuring the residual quantity of doxycycline in the tissue samples collected in the step 2; 4, calculating pharmacokinetic parameters including area under a drug time curve, absorption half-life period, elimination half-life period, peak reaching time, peak concentration, apparent distribution volume and clearance rate; and 5, determining a doxycycline administration scheme according to a calculation formula 1. According to the determination method, the medication efficiency and medication safety can be improved.

Description

technical field [0001] The invention relates to the field of pharmacological effects of aquatic medicines, in particular to a method for determining the administration regimen of doxycycline to Citrobacter freundii of Procambarus clarkii under different administration modes. Background technique [0002] Procambarus clarkii, also known as crayfish, is one of the important species of crustaceans cultured in my country, with an annual output of 1,638,700 tons, which plays a pivotal role in my country's aquaculture. With the continuous expansion of the scale of farming, crayfish diseases occur frequently, and effective drug treatment is required. The mode of administration is directly related to the absorption rate of the drug, thereby affecting the metabolism, distribution and excretion of the drug, and plays a key role in the effectiveness of the drug. [0003] Citrobacter freundii belongs to the family Enteridae, Citrobacter genus, is a gram-negative bacterium, aerobic or f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16H20/10G16H70/40G16C20/50
CPCG16H20/10G16H70/40G16C20/50
Inventor 胥宁艾晓辉董靖刘永涛杨移斌周顺杨秋红
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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