Method for determining dosing schemes of doxycycline to Citrobacter freundii of procambarus clarkia in different dosing modes
A technology of citric acid for shrimp and Crayfish, which is applied in the field of drug efficacy of aquatic products, can solve problems such as economic loss, meningitis, enteritis and sepsis, and death of farmed animals, so as to reduce economic loss, improve drug efficiency and improve efficiency. The effect of drug safety
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[0029] 1. Experimental animals
[0030] 300 healthy crayfish (average body weight 19.2±5.5g) were purchased from the experimental base of the Yangtze River Fisheries Research Institute of the Chinese Academy of Fishery Sciences. The test water was continuously aerated with an oxygen pump to keep the dissolved oxygen in the water greater than 4.8mg / L, the pH was 7.2-7.8, and the temperature was divided into two groups at 24±0.5°C. Before the experiment, the crayfish were kept in a glass aquarium for one week.
[0031] 2. Drugs and reagents
[0032] Drugs: Doxycycline powder was purchased from Hubei Aibo Technology Co., Ltd., with a content greater than 98%;
[0033] The standard product doxycycline was purchased from Dr. Ehrenstorfer Company in Germany, and its content was greater than 99%.
[0034] Reagent:
[0035] EDTA-Na2, sodium chloride, magnesium sulfate, sodium dihydrogen phosphate and sodium citrate were purchased from Shanghai Sinopharm Group, analytically pure; C...
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