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Method for constructing animal model of acute kidney injury after acute myocardial infarction

A technology for acute myocardial infarction and acute kidney injury, which is applied in the field of medical animal models, can solve problems such as low success rate, complicated operation, and long time consumption, and achieve the effect of improving the success rate of modeling

Pending Publication Date: 2022-05-13
GUANGDONG GENERAL HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current animal model of acute kidney injury after acute myocardial infarction uses a myocardial ischemia-reperfusion model, which uses a coronary ischemia-reperfusion method. The operation is complicated, time-consuming, and the success rate is low. Higher requirements are placed on the operation of researchers
In addition, the currently used animal model of acute kidney injury after acute myocardial infarction does not use contrast agents, which is not in line with the actual clinical situation

Method used

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  • Method for constructing animal model of acute kidney injury after acute myocardial infarction
  • Method for constructing animal model of acute kidney injury after acute myocardial infarction
  • Method for constructing animal model of acute kidney injury after acute myocardial infarction

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1 Construction of an animal model of acute kidney injury after acute myocardial infarction in the coronary ischemia-reperfusion (Ischia reperfusion injury, IRI) + contrast medium (Contrast medium, CM) group.

[0034] A method for constructing an animal model of acute kidney injury after acute myocardial infarction in this example: rats were fasted for 24 hours before surgery, and 1 hour before surgery, rats were injected with iopromide into the tail vein, and the injection volume was 15 ml / kg. Rats were anesthetized by injecting pentobarbital sodium, and the injection volume of pentobarbital sodium was 15 mg / kg. After anesthesia, the limbs and head were fixed, and an indwelling needle was implanted through the tail vein as an interventional venous access, and 1ml of anticoagulant blood was taken from the tail vein. After connecting the small animal ECG machine, the basic ECG can be seen. Perform tracheal intubation with a pen-shaped intravenous indwelling needle...

Embodiment 2

[0035] Example 2 Construction of an animal model of acute kidney injury after acute myocardial infarction in the coronary ischemia-reperfusion (Ischia reperfusion injury, IRI) group.

[0036]The method for constructing an animal model of acute kidney injury after acute myocardial infarction in this example: rats were fasted for 24 hours before operation, and 1 hour before operation, rats were injected with normal saline in the tail vein, and the injection volume was 15 ml / kg. Rats were anesthetized by injecting pentobarbital sodium, and the injection volume of pentobarbital sodium was 15 mg / kg. After anesthesia, the limbs and head were fixed, and an indwelling needle was implanted through the tail vein as an interventional venous access, and 1ml of anticoagulant blood was taken from the tail vein. After connecting the small animal ECG machine, the basic ECG can be seen. Perform tracheal intubation with a pen-shaped intravenous indwelling needle and connect to the ventilator. I...

Embodiment 3

[0037] Example 3 Construction of an animal model of acute kidney injury after acute myocardial infarction in the contrast medium (Contrast medium, CM) group.

[0038] A method for constructing an animal model of acute kidney injury after acute myocardial infarction in this example: rats were fasted for 24 hours before surgery, and 1 hour before surgery, rats were injected with iopromide into the tail vein, and the injection volume was 15 ml / kg. Rats were anesthetized by injecting pentobarbital sodium, and the injection volume of pentobarbital sodium was 15 mg / kg. After anesthesia, the limbs and head were fixed, and an indwelling needle was implanted through the tail vein as an interventional venous access, and 1ml of anticoagulant blood was taken from the tail vein. After connecting the small animal ECG machine, the basic ECG can be seen. Perform tracheal intubation with a pen-shaped intravenous indwelling needle and connect to the ventilator. If the chest can be seen to fluct...

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Abstract

The invention discloses a construction method of an animal model of acute kidney injury after acute myocardial infarction, and belongs to the technical field of medical animal models. The construction method of the animal model of acute kidney injury after acute myocardial infarction comprises the following steps: (1) applying a contrast agent to an experimental animal; (2) separating a third rib and a fourth rib of the experimental animal in the step (1), finding a vertical walking position of a left front descending branch, blocking blood flow of the left front descending branch, and observing waveform change of an electrocardiograph of the experimental animal; and (3) performing reperfusion on the experimental animal in the step (2). According to the construction method of the animal model of the acute kidney injury after the acute myocardial infarction, the model which is closer to the clinical model of the acute kidney injury after the acute myocardial infarction is successfully constructed through caudal vein injection of a clinically common iopromide contrast agent and optimization of the modeling operation process and modeling conditions, and the modeling success rate is improved.

Description

technical field [0001] The invention belongs to the technical field of medical animal models, and in particular relates to a method for constructing an animal model of acute kidney injury after acute myocardial infarction. Background technique [0002] Acute myocardial infarction (AMI, acute myocardial infraction) is myocardial necrosis caused by acute and persistent coronary ischemia and hypoxia. Clinically, there are often severe and persistent substernal pain, which cannot be completely relieved by rest and nitrates, accompanied by increased serum myocardial enzyme activity and progressive ECG changes, which may be complicated by arrhythmia, shock or heart failure. The clinical feature of acute kidney injury (AKI, acute kidney injury) is a sharp decline in renal function within several days. The main etiology is renal ischemia-reperfusion injury, sepsis, nephrotoxic drug attack and post-renal obstruction. Microscopic pathology of acute kidney injury usually manifests as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00A61D7/00
CPCA61D1/00A61D7/00
Inventor 刘勇李焕强麦梓玲陈世群刘津谭宁陈纪言
Owner GUANGDONG GENERAL HOSPITAL