Pichia pastoris, leavening agent and application of pichia pastoris and leavening agent in degradation of vomitoxin or preparation of fermented feed
A technology for vomitoxin and fermented feed, applied in the field of microorganisms, can solve problems such as vomitoxin pollution, and achieve the effects of simple production process, improved nutritional value and high degradation rate
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preparation example Construction
[0046] In a third aspect, the present invention provides a method for preparing a starter, the method comprising: fermenting and culturing the above-mentioned Pichia pastoris in a yeast fermentation medium.
[0047] Preferably, the method comprises: cultivating the above-mentioned Pichia pastoris in a yeast fermentation medium, and making the OD 600 Reach 5-11 (preferably 10-11), the fermented liquid that obtains is starter. It should be understood that the fermentation broth can also be post-treated to obtain different forms of fermentation agents, which can be selected by those skilled in the art according to needs, and will not be repeated here.
[0048] The yeast fermentation medium may contain saccharification liquid and corn steep liquor. In terms of dry matter, the content of the saccharification liquid in the yeast fermentation medium is 20-50 g / L, and the content of corn steep liquor is 2-4 g / L.
[0049] Wherein, the preparation method of the saccharification liquid ...
Embodiment
[0084] The culture medium formulation involved in the following examples is as follows:
[0085] Corn steep liquor: dry matter content 40% by weight, pH 4-7.
[0086] The Aspergillus niger residue is the Aspergillus niger residue from COFCO Biochemical Energy (Yum) Co., Ltd., with a water content of 77%.
[0087] Gypsum was gypsum from COFCO Biochemical Energy (Yumshu) Co., Ltd., with a water content of 27%.
[0088] YPD medium: yeast extract powder 10g / L, peptone 20g / L, glucose 20g / L, sterilized at 121°C for 20min.
[0089] MRS medium: peptone 10g / L, beef extract 10g / L, yeast extract powder 5g, K 2 HPO 4 2g / L, diammonium citrate 2g / L, sodium acetate 5g / L, glucose 20g / L, MgSO 4 ·7H 2 O 0.58g / L, MnSO 4 4H 2 O 0.25g / L, sterilized at 121°C for 20min.
[0090] LB medium: peptone 10g / L, yeast extract powder 5g / L, NaCl 10g / L, sterilized at 121°C for 20min.
[0091] Corn steep liquor medium: After diluting corn steep liquor twice, adjust the pH to 5 with 5mol / L NaOH, centri...
preparation example
[0103] This preparation example is used to illustrate the activation method of the bacterial strain.
[0104] (1) Activation of Pichia pastoris: Pichia pastoris was inoculated in YPD medium, and cultured on a shaker at 200 r / min at 30° C. for 12 hours to obtain an activation solution.
[0105] (2) Activation of lactic acid bacteria: Pichia pastoris was inoculated in MRS medium, and cultured on a shaker at 200 r / min at 30° C. for 12 hours to obtain an activated solution.
[0106] (3) Activation of bacillus: inoculate the bacillus in LB medium, and cultivate and activate on a shaker at 200 r / min and 37° C. for 12 hours to obtain an activation solution.
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