SgRNA for improving drought tolerance and saline-alkaline tolerance of cotton and application of sgRNA

A technology for salt and alkali tolerance and cotton, applied in the field of sgRNA, can solve the problems of undiscovered cotton drought tolerance and salt and alkali tolerance, and achieve the effects of high gene editing efficiency, improved salt and alkali tolerance, and improved cotton fiber quality.
CN114480415AActive Publication Date: 2022-05-13THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
Publication Date
2022-05-13

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Abstract

The invention belongs to the field of plant genetic engineering, and particularly discloses sgRNA for improving drought tolerance and saline-alkaline tolerance of cotton, and a target sequence of the sgRNA is composed of a nucleotide sequence as shown in SEQ ID No.1. The invention also discloses application of the sgRNA in improving drought tolerance and saline-alkaline tolerance of cotton. The sgRNA is designed according to conserved regions of all GI homologous genes of upland cotton and sea island cotton, different GI genes can be edited independently or simultaneously, and the gene editing efficiency is high; secondly, compared with a wild plant, the drought tolerance and saline-alkaline tolerance of a cotton mutant plant obtained by editing a single Gigantea (GI) gene by utilizing the sgRNA disclosed by the invention are remarkably improved; in addition, the sgRNA silencing expression of the GI gene is of great significance for increasing the cotton yield and improving the cotton fiber quality.
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Description

technical field

[0001] The invention belongs to the field of plant genetic engineering, and in particular relates to an sgRNA for improving the drought and saline-alkali tolerance of cotton; it also relates to the use of the sgRNA in improving the drought- and saline-alkali tolerance of cotton. Background technique

[0002] Cotton is an important raw material for the textile industry. With the transfer of my country's cotton planting areas to Xinjiang and other northwest regions, drought and saline-alkali land hazards have become the main factors limiting local cotton production. Although cotton is a relatively drought- and saline-alkali-tolerant crop, soil salinity-alkali content exceeding 0.3% or lack of water during the critical period of growth and development may lead to more than 90% reduction in cotton yield. Therefore, it is the most economical way to solve the drought-resistant or saline-alkali-resistant cotton varieties by studying the mechanism of cotton tolerance...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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