Sample pretreatment method

A sample and reagent technology, applied in the field of sample detection pre-processing, can solve the problems of affecting the detection results, time-consuming operation, and difficulty in stratification and stratification, and achieve the effect of reducing environmental pollution and improving detection requirements.

Pending Publication Date: 2022-05-27
GUANGZHOU ANNUO FOOD SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conventional method is to add 1 to 2ml of n-hexane to the sample solution after drying to remove grease and impurities. However, since the sample solution is dissolved in an organic solvent, and n-hexane is easily soluble in organic solvents, it may be difficult to separate or When the stratification is not obvious, the operation takes a long time
Moreover, when taking the test solution, it is easy to get n-hexane, which will affect the test results.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A sample pretreatment method, comprising the following steps:

[0022] 1) Weigh 3 g of fish meat sample, add 3 mL of reagent A, then add 1 g of anhydrous sodium sulfate, then add 4 mL of acetonitrile, shake for 2 min, and centrifuge at 4000 r / min for 5 min; wherein, reagent A includes hydroxylamine hydrochloride in a mass ratio of 1:11 mixed solution with sodium chloride;

[0023] 2) Take 2 mL of the centrifuged supernatant, add 100 μL of reagent B, and dry it with a water-bath nitrogen blower at 65°C; reagent B is prepared by adding 20 g of hydroxylamine chloride to 1000 mL of water;

[0024] 3) Add 0.3 mL of Reagent C after nitrogen blowing in a water bath, and after mixing, a detection solution is obtained; Reagent C includes 4.7 g of buffer, 1.2 g of tris(hydroxymethyl) aminomethane (Tris-HCl), 8.5 g of chloride A mixed solution of sodium and 4.5g of surfactant. The buffer is 4-hydroxyethylpiperazineethanesulfonic acid (HEPES), and the surfactant is a condensate o...

Embodiment 2

[0026] A sample pretreatment method, comprising the following steps:

[0027] 1) Weigh 3 g of fish meat sample, add 3 mL of reagent A, then add 1.5 g of anhydrous sodium sulfate, then add 4 mL of acetonitrile, shake for 2 min, and centrifuge at 4000 r / min for 5 min; wherein, reagent A includes hydrochloric acid with a mass ratio of 1:11 Mixed solution of hydroxylamine and sodium chloride;

[0028] 2) Take 2 mL of the centrifuged supernatant, add 100 μL of reagent B, and dry it with a water-bath nitrogen blower at 65°C; reagent B is prepared by adding 20 g of hydroxylamine chloride to 1000 mL of water;

[0029] 3) Add 0.3 mL of Reagent C after nitrogen blowing in a water bath, and after mixing, a detection solution is obtained; Reagent C includes 4.7 g of buffer, 1.2 g of tris(hydroxymethyl) aminomethane (Tris-HCl), 8.5 g of chloride A mixed solution of sodium and 4.5g of surfactant. The buffer is 4-hydroxyethylpiperazineethanesulfonic acid (HEPES), and the surfactant is a co...

Embodiment 3

[0031] A sample pretreatment method, comprising the following steps:

[0032] 1) Weigh 3 g of fish meat sample, add 3 mL of reagent A, then add 2 g of anhydrous sodium sulfate, then add 4 mL of acetonitrile, shake for 2 min, and centrifuge at 4000 r / min for 5 min; wherein, reagent A includes hydroxylamine hydrochloride in a mass ratio of 1:11 mixed solution with sodium chloride;

[0033] 2) Take 2 mL of the centrifuged supernatant, add 100 μL of reagent B, and dry it with a water-bath nitrogen blower at 65°C; reagent B is prepared by adding 20 g of hydroxylamine chloride to 1000 mL of water;

[0034] 3) Add 0.3 mL of Reagent C after nitrogen blowing in a water bath, and after mixing, a detection solution is obtained; Reagent C includes 4.7 g of buffer, 1.2 g of tris(hydroxymethyl) aminomethane (Tris-HCl), 8.5 g of chloride A mixed solution of sodium and 4.5g of surfactant. The buffer is 4-hydroxyethylpiperazineethanesulfonic acid (HEPES), and the surfactant is a condensate o...

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PUM

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Abstract

The invention discloses a sample pretreatment method which comprises the following steps: 1) weighing a sample, adding a reagent A, then adding anhydrous sodium sulfate, then adding an organic solvent, oscillating and centrifuging; wherein the reagent A comprises a mixed solution of a reducing agent and sodium chloride; 2) taking the centrifuged supernate, adding a reagent B, and carrying out water bath nitrogen blowing treatment; the reagent B is an oxidant; 3) adding a reagent C after water bath nitrogen blowing treatment, and mixing to obtain a detection solution; the reagent C comprises a mixed solution of a buffer solution, sodium chloride and a surfactant. According to the sample pretreatment method, toxic n-hexane is replaced by nontoxic anhydrous sodium sulfate, the anhydrous sodium sulfate also has the effects of removing oil and impurities, but the anhydrous sodium sulfate is not mutually soluble with an organic solvent and can be thoroughly separated from an organic layer through centrifugation after centrifugation, and the subsequent detection result is not influenced. According to the sample pretreatment method, n-hexane does not need to be used, the environmental pollution can be reduced, and the detection requirement is improved.

Description

technical field [0001] The invention relates to the technical field of sample detection pretreatment, in particular to a sample pretreatment method. Background technique [0002] It is necessary to remove impurities and wash the surface fat of the sample before the test, so as to avoid the follow-up test results. The conventional method is to add 1 to 2 ml of n-hexane to the sample solution after drying to remove grease and impurities. However, since the sample solution is dissolved in an organic solvent, and n-hexane is easily soluble in organic solvents, it may be difficult to delaminate or dissolve. When the layering is not obvious, the operation takes a long time. Moreover, when taking the test solution, n-hexane is easy to be taken, which affects the test results. Furthermore, n-hexane is a colorless liquid with low toxicity and a weak characteristic odor. Long-term exposure can cause chronic poisoning symptoms such as headache, dizziness, fatigue, and numbness in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34G01N1/40
CPCG01N1/34G01N1/4022
Inventor 温俊梅王军范彬杨鹏博黄凤
Owner GUANGZHOU ANNUO FOOD SCI & TECH CO LTD
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