A kind of probiotic fermentation product for relieving depression and calming heart and its preparation method and application
A technology of probiotics and fermented products is applied in the field of probiotics fermented products for relieving depression and calming heart and the preparation thereof, and can solve the problems of easy accumulation of toxins, low bioavailability, strong toxicity and the like
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Embodiment 1
[0109] 1. Preparation of culture medium
[0110] 1) Basal medium
[0111] 6.0g yeast hydrolyzate, 2.0g corn steep liquor, 3.0g peanut flour, 10.0g molasses, 3.0g KCl, 0.1g ZnSO 4 ·8H 2 O, 2g FeSO 4 ·4H 2 O, 8.0g CaCO 3 Dissolve in 1L of purified water, adjust pH to 5.8, and prepare basal medium (when preparing solid medium, add 15g of agar).
[0112] 2) Fermentation medium
[0113] Put the fruit Chinese medicine composition into a CO 2 The immersion temperature is 20 ℃, the time is 3d, and then crushed and squeezed to obtain the raw material juice base; the purified water is added to the raw juice base to adjust the size, and the volume of the raw juice base and purified water is controlled. The ratio is 1:4, adding S0 2 80ml / L and 0.5% baking soda to prepare the raw material juice of fruit traditional Chinese medicine composition.
[0114] Add 50g molasses, 6g yeast hydrolyzate, 2g ferric sulfate and 8.0g CaCO to 1L of the above raw material juice 3 , adjust the pH...
Embodiment 2
[0130] 1. Preparation of culture medium
[0131] 1) Basal medium
[0132] 10.0g yeast hydrolyzate, 3.0g corn steep liquor, 4.0g peanut flour, 10.0g molasses, 2.0g KCl, 0.3g ZnSO 4 ·8H 2 O, 2.2g FeSO 4 ·4H 2 O, 10.0g CaCO 3 Dissolve in 1 L of purified water, adjust the pH to 6.0, and prepare a basal medium (when preparing a solid medium, add 15 g of agar).
[0133] 2) Fermentation medium
[0134] Put the fruit Chinese medicine composition into a CO 2 The immersion temperature is 22 ℃, the time is 4d, and then crushed and squeezed to obtain the raw material juice base material; purified water is added to the raw material juice base material for slurrying, and the volume of the raw material juice base material and purified water is controlled. The ratio is 1:3, adding S0 2 80ml / L and 0.5% baking soda to prepare the raw material juice of fruit traditional Chinese medicine composition.
[0135] Add 60g molasses, 10g yeast hydrolyzate, 2.2g ferric sulfate and 10.0g CaCO to...
Embodiment 3
[0151] 1. Preparation of culture medium
[0152] 1) Basal medium
[0153] 10.0g yeast hydrolyzate, 3.0g corn steep liquor, 4.0g peanut flour, 10.0g molasses, 2.0g KCl, 0.3g ZnSO 4 ·8H 2 O, 2.2g FeSO 4 ·4H 2 O, 10.0g CaCO 3 Dissolve in 1 L of purified water, adjust the pH value to 6.0, and prepare a basal medium (when preparing a solid medium, add 15 g of agar).
[0154] 2) Fermentation medium
[0155] Put the fruit Chinese medicine composition into a CO 2 The immersion temperature is 24 ℃, the time is 5d, and then crushed and squeezed to obtain the raw juice base material; purified water is added to the raw juice base material for slurrying, and the volume of the raw juice base material and purified water is controlled. The ratio is 1:2, adding S0 2 80ml / L and 0.5% baking soda to prepare the raw material juice of fruit traditional Chinese medicine composition.
[0156] Add 60g molasses, 10g yeast hydrolyzate, 2.2g ferric sulfate and 10.0g CaCO to 1L of the above raw ...
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