Selenium yeast hydrolyzate and its preparation method and application

A technology of selenium yeast and hydrolyzate, which is applied in the production of yeast selenium and the field of biological enzymolysis, which can solve the problems of large environmental impact and reduced product yield, and achieve the effects of high content of functional ingredients, increased absorption rate, and high yield

Active Publication Date: 2021-12-07
ANGELYEAST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method adds ethyl acetate as an autolysis accelerator, but ethyl acetate is a highly volatile substance and has a greater impact on the environment
And this method obtains product completely soluble by centrifugation with supernatant, removes a large amount of functional components, and the product yield that finally obtains can be greatly reduced

Method used

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  • Selenium yeast hydrolyzate and its preparation method and application
  • Selenium yeast hydrolyzate and its preparation method and application

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preparation example Construction

[0045] The preferred preparation method of selenium yeast hydrolyzate in the present invention comprises the following steps:

[0046] (1) The selenium yeast milk is stored at 2-8° C., and the pH of the selenium yeast milk is adjusted to 4-5, preferably 4.5-4.8 by adding 2%-6% citric acid relative to the weight of yeast dry matter.

[0047]If the amount of citric acid is too high, the content of crude protein, ammonia nitrogen, and acid-soluble protein will decrease. At the same time, the addition of excessive citric acid will increase the amount of sodium hydroxide, which will also indirectly reduce crude protein, acid-soluble protein, and ammonia nitrogen. In the present invention, it is preferable to add citric acid accounting for 3-4% of the weight of yeast dry matter. The amount of citric acid added within this range can reduce the risk of microbial contamination during material transfer and heating, and can promote some functional indicators of the product.

[0048] (2) ...

Embodiment 1

[0067] (1) Store the separated selenium yeast milk at 3-4° C., and add 2% citric acid to adjust the pH to 4.9-5.0.

[0068] (2) Rapidly heat up the selenium yeast preserved in step (1) by means of short-term heat exchange with 93-95°C steam, heat up to 37.5-39.5°C for 1 hour; heat up to 46.5-48.5°C for 1 hour, then heat up to 51.5-53.5°C.

[0069] (3) Keep the temperature of the yeast milk heated up in step (2) at 51.5-53.5° C., and keep it warm for 6 hours.

[0070] (4) Adjust the pH of the yeast milk after step (3) for 6 hours to 5.6-5.7, heat up to 55.5-57.5°C, then add 0.13% papain, 0.13% neutral protease, and 0.13% alkaline protease to carry out enzymatic hydrolysis reaction 10h.

[0071] (5) Heating the hydrolyzate obtained in step (4), incubating at 73-75° C. for 1 h, evaporating and concentrating to a solid content of 40%, and spray-drying to obtain the selenium yeast hydrolyzate.

[0072] The spray drying conditions are: the inlet air temperature is controlled at 1...

Embodiment 2

[0074] (1) Store the separated selenium yeast milk at 2-3°C, and add 3% citric acid to adjust the pH to 4.7-4.8.

[0075] (2) Rapidly raise the temperature of the selenium yeast preserved in step (1) by means of short-term heat exchange with steam at 90-92°C, raise the temperature to 35.5-37.5°C, and keep it warm for 1.5h; heat it up to 45.5-47.5°C and keep it warm for 1.5h , and then heated to 53.5-55.5 ° C.

[0076] (3) Keep the temperature of the yeast milk heated up in step (2) at 53.5-55.5° C., and keep it warm for 10 hours.

[0077] (4) Adjust the pH of the autolyzed yeast milk in step (3) to 6.1-6.2, raise the temperature to 57.5-59.5°C, and then add 0.3% papain, 0.3% alkaline protease and 0.3% flavor protease to carry out the enzymatic hydrolysis reaction for 6 hours .

[0078] (5) Heating the hydrolyzate obtained in step (5) to 70-72° C., keeping it warm for 2 hours, evaporating and concentrating to a solid content of 37%, and spray-drying to obtain the selenium yea...

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Abstract

The invention relates to a selenium yeast hydrolyzate and its preparation method and application. The selenium yeast hydrolyzate of the present invention contains protein, amino acid nitrogen, acid-soluble protein and selenomethionine, wherein the content of amino acid nitrogen is 2-3wt%, and the content of acid-soluble protein is 35-45wt%. Add citric acid to the selenium yeast milk for autolysis treatment and enzymolysis treatment, and the enzymes added in the enzymolysis treatment include papain, neutral protease, alkaline protease and flavor protease. The product of the invention is easy to be produced on a large scale, and the selenium yeast hydrolyzate prepared by the method of the invention has a high content of functional components, and the absorption rate of the selenium element in animals is greatly increased, and can be used as a feed product additive.

Description

technical field [0001] The invention relates to a preparation method and a product of a selenium yeast hydrolyzate, belonging to the technical fields of yeast selenium production and biological enzymolysis. Background technique [0002] Selenium is an indispensable trace element in animal physiology. As a stimulator of animal immune system, it can promote antibody production, strengthen animal immune system and anti-infection ability, and improve animal reproductive performance. Selenium deficiency can lead to decreased sperm count and motility and increased sperm deformity rate in male animals. At present, selenium added in the field of feed exists in two forms: inorganic selenium and organic selenium. Inorganic selenium mainly uses inorganic compounds such as sodium selenite as selenium sources. Such products have low absorption rate and high toxicity. To achieve the desired effect, it also has a great destructive effect on the environment and a potential threat to animal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/06C12R1/865
CPCA23K20/30A23K50/30A23K50/75C12N1/06
Inventor 徐智鹏俞学锋李知洪姚鹃胡骏鹏戴晋军曾雨雷陈中平贺淼
Owner ANGELYEAST CO LTD
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