Application of luteolin in preparation of health care products or medicines for improving hypoxia tolerance of organisms

A technology of luteolin and tolerance, applied in the directions of drug combination, medical preparations containing active ingredients, pharmaceutical formulas, etc.

Pending Publication Date: 2022-06-03
INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether luteolin can improve the body's ability to tolerate hypoxia has not yet been reported.

Method used

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  • Application of luteolin in preparation of health care products or medicines for improving hypoxia tolerance of organisms
  • Application of luteolin in preparation of health care products or medicines for improving hypoxia tolerance of organisms
  • Application of luteolin in preparation of health care products or medicines for improving hypoxia tolerance of organisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 The effect of luteolin on the survival time of mice under normal pressure airtight hypoxia

[0017] 1. Experimental animals

[0018] Forty male Kunming mice of SPF grade, weighing 18-20 g, were purchased from Beijing Weitong Lihua Laboratory Animal Technology Co., Ltd.

[0019] 2. Experimental Drugs

[0020] (1) 0.5% sodium carboxymethyl cellulose solution:

[0021] Take 5g of sodium carboxymethylcellulose powder, add ultrapure water to 1000ml, and use a magnetic stirrer to accelerate the dissolution.

[0022] (2) 25mg / kg, 50mg / kg, 100mg / kg luteolin solution:

[0023] The luteolin powder was weighed according to the weight of the mice and dissolved in 0.5% sodium carboxymethyl cellulose solution to prepare different doses of luteolin solution.

[0024] 3. Grouping and administration of experimental animals

[0025] Forty Kunming mice of 18-20 g were adaptively reared for 7 days. After 7 days, the mice were randomly divided into groups according to their ...

Embodiment 2

[0043] Example 2 The regulatory effect of luteolin on serum oxidative and antioxidant levels in mice

[0044] 1. Collection of Serum

[0045] After the mice died of airtight hypoxia, the eyeball blood was collected immediately, and the collected eyeball blood was allowed to stand at room temperature for 30 minutes to make it coagulate, and then centrifuged at 3000 r / min and 4 °C for 10 minutes, and the upper serum was taken. Transfer to a 1.5ml EP tube for later use.

[0046] 2. Determination of serum glutathione peroxidase (GSH-Px) activity

[0047]The glutathione peroxidase (GSH-Px) assay kit (purchased from Nanjing Jiancheng Bioengineering Institute) was used to detect the activity of GSH-Px in serum. All operations were carried out according to the instructions, and the following formula was used to calculate serum GSH-Px. Px Vitality.

[0048]

[0049] 3. Determination of serum total antioxidant capacity (T-AOC)

[0050] Total antioxidant capacity (T-AOC) assay kit...

Embodiment 3

[0056] Example 3 The regulatory effect of luteolin on the oxidation and antioxidant levels of important organs in mice

[0057] 1. Collection of tissue samples

[0058] After the mice died of airtight hypoxia, the brains, hearts and livers of the mice were immediately taken, washed with normal saline and used for later use.

[0059] 2. Lysis of tissue samples and determination of protein concentration

[0060] The BCA protein concentration assay kit (Soleibao Biotechnology Co., Ltd.) was used to measure the tissue protein concentration, and all operations were carried out according to the instructions.

[0061] 3. Determination of Glutathione Peroxidase (GSH-Px) Activity in Tissue

[0062] The glutathione peroxidase (GSH-Px) assay kit (purchased from Nanjing Jiancheng Bioengineering Institute) was used to detect the activity of GSH-Px in the tissue. All operations were carried out according to the instructions, and the following formula was used to calculate the tissue GSH-P...

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Abstract

The invention discloses application of luteolin in preparation of health care products or medicines for improving hypoxia tolerance of organisms, experiments prove that the luteolin can obviously prolong the closed hypoxia survival time of the organisms, has the effect of improving the hypoxia tolerance of the organisms, and can be used for preparing health care products or medicines for improving the hypoxia tolerance of the organisms. And the luteolin can significantly improve the vitality of glutathione peroxidase (GSH-Px) and total superoxide dismutase (T-SOD) in serum and the vitality of total superoxide dismutase (T-SOD) in cardiac muscle tissues in a closed anoxic state, reduces the content of malondialdehyde (MDA) in liver tissues, and has the function of improving oxidative stress of an organism in an anoxic state.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of luteolin in the preparation of health-care products or medicines for improving the hypoxia tolerance of the body. Background technique [0002] Hypoxia means that the oxygen required for the body's life activities cannot be adequately supplied. The formation of hypoxia in the body mainly includes the following three aspects: first, the oxygen content of the external environment is reduced, such as plateau and aviation hypoxia; second, the normal oxygen cannot fully reach the body due to diseases, etc.; third, the oxygen consumption required by the body's activities The amount exceeds the physiological mobilization ability, resulting in insufficient relative oxygen supply, such as strenuous exercise and excessive labor. How to improve the body's ability to resist hypoxia and how to effectively intervene the damage caused by hypoxia to the human body are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P39/00
CPCA61K31/352A61P39/00
Inventor 陈照立刘伟丽蒲玲玲徐洪宝王新兴王天辉李冉王紫瑈艾崇毅
Owner INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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