Ackermansiella muciniphila microecological preparation and application thereof in laying hens

A technology of mucin and bacterial agents, applied in the biological field, can solve the problems of eggshell quality decline, production performance and egg quality decline, and quantity increase, so as to achieve the effect of improving egg quality and broad market application prospects

Active Publication Date: 2022-06-03
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The egg production of diseased chickens will be affected, and the production performance and egg quality will decline. Especially for aged layer hens, the quality of

Method used

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  • Ackermansiella muciniphila microecological preparation and application thereof in laying hens
  • Ackermansiella muciniphila microecological preparation and application thereof in laying hens
  • Ackermansiella muciniphila microecological preparation and application thereof in laying hens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1. Preparation of Akkermansia muciniphila probiotics

[0067] 1. Preparation of live bacteria agent of Akkermansia muciniphila BAA-835

[0068] Under sterile conditions, resuspend the lyophilized powder of Akkermansia muciniphila strain BAA-835 with sterile water, suck the bacterial suspension, and put it into 1-2 Columbia blood plates. 200 μL / piece, after evenly coating, the plate was placed in an anaerobic incubator (Whitley DG250 Anaerobic Workstation, Bailai Puhui (Beijing) Technology Co., Ltd.), cultured at 37°C for 5-7 days, and the anaerobic gas composition was N 2 :CO 2 :H 2 =80:10:10, then single colonies were picked in the modified GAM liquid medium (monthly peptone 15g / L; tryptone peptone 10g / L; soybean peptone 3g / L; yeast extract powder 5g / L; beef powder 2g / L ; Digestive Serum Powder 13.5g / L; Beef Liver Extract Powder 1.2g / L; Glucose 3g / L; Potassium Dihydrogen Phosphate 2.5g / L; Sodium Chloride 3g / L; Soluble Starch 0.3g / L; L-cysteine Amino acid 0....

Embodiment 2

[0076] Example 2. Application of Akkermansia muciniphila BAA-835 live bacteria and inactivated bacteria in relieving fatty liver of laying hens

[0077] 1. Experimental method:

[0078] The model of laying hens selected in this example is: fatty liver model induced by high-energy and low-protein diet (HFD). 80 28-week-old Hailan brown laying hens (Zhuozhou Bo Nong Poultry Co., Ltd.) with similar body weight (2.010±0.10kg) were randomly divided into 4 groups: control group (ND), high-fat model group (HFD) , Akkermansia muciniphila live relief group (HFD_LA) and Akkermansia mucinophila pasteurized relief group (HFD_PA), 20 in each group. The implementation site of this example is the poultry test base of China Agricultural University (Zhuozhou, Hebei), including a pre-feeding period of 1 week and a normal feeding period of 18 weeks. All groups were fed the basal diet during the pre-feeding period.

[0079] Control group (ND): The basal diet was fed during the normal feeding p...

Embodiment 3

[0117] Embodiment 3. Application of Akkermansia mucinophilus BAA-835 freeze-dried powder on aged laying hens

[0118] 1. Experimental Design

[0119] In this example, 200 64-week-old healthy Hailan brown laying hens were selected, and blood was collected from the fin vein after fasting for 8 hours, the serum was separated, and the content of TC and TG in the serum was detected (Beijing Jinhai Keyu Biotechnology Development Co., Ltd.). According to the content of serum TC, TG and egg production rate, 140 chickens were selected and randomly divided into two groups, with 7 replicates in each group, and 10 chickens in each replicate to ensure the initial body weight of the two groups (control group: 2.17± 0.12kg; test group: 2.16±0.12kg), egg production rate (control group: 89.6±5.67%; test group: 89.6±5.48%), serum TC (control group: 3.71±0.42mmol / L; test group: 3.71 ±0.40mmol / L), TG (control group: 8.59±2.80mmol / L; test group: 8.59±2.74mmol / L) levels were consistent. The imple...

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Abstract

The invention discloses an Ackermania muciniphila microecological preparation and an application of the Ackermania muciniphila microecological preparation in laying hens. Three micro-ecological microbial agents, including a viable bacterium agent, a pasteurization bacterium agent and a freeze-dried powder bacterium agent, are drenched to the hyline brown laying hens and added into feed, and the result shows that the microbial agents of the ackermansiella muciniphila can reduce the increase of blood fat and liver fat levels caused by high-fat diet of the hyline brown laying hens, so that the content of the ackermansiella muciniphila in the hyline brown laying hens is increased, and the content of the ackermansiella muciniphila in the hyline brown laying hens is increased. In-vivo fat deposition is reduced, and the egg quality is improved. The Ackermania muciniphila microecological preparation (microbial inoculum) provided by the invention is comprehensive in function, non-toxic and harmless, and the pasteurization inactivated bacteria microbial inoculum, the freeze-dried powder microbial inoculum and the viable bacteria microbial inoculum have the same action effect in the aspects of preventing/relieving fatty liver of laying hens and improving egg quality, and have greater advantages in the aspects of storage and addition modes. The invention provides a basis for the Ackermania muciniphila as a novel microecological preparation in the laying hen breeding industry.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a microecological preparation of Akkermansia muciniphila and its application on laying hens. Background technique [0002] Fatty liver syndrome is a common and frequent nutritional metabolic disease in caged laying hens. The main reason is that nutritional disorders in laying hens lead to endocrine disorders and metabolic disorders in the body, resulting in excessive fat deposition. The sick chicken likes to lie down, the abdomen is swollen and sagging, soft to the touch, there is a lot of fat accumulation, lethargy, and lack of energy. The egg production of diseased hens will be affected, and the production performance and egg quality will decline, especially for old laying hens, the quality of egg shells will decline, and the number of broken shell eggs, soft shell eggs, and sand shell eggs will increase, causing huge losses to the laying hen industry. economic losses. [0003] E...

Claims

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Application Information

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IPC IPC(8): A61K35/74C12N1/20A61P3/00A61P43/00A61P3/04A61P1/16A61P3/06C12R1/01
CPCA61K35/741C12N1/20A61P3/00A61P43/00A61P3/04A61P1/16A61P3/06Y02P60/87Y02A40/70
Inventor 刘丹胡永飞魏福晓金晓露
Owner CHINA AGRI UNIV
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