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Separation method of phenolic acid components in traditional Chinese medicine

A separation method and technology of phenolic acid, applied in the field of analytical chemistry, can solve the problems of complex composition, insufficient separation selectivity, limited peak capacity, etc., and achieve the effects of good separation selectivity, easy operation and simple analysis method.

Active Publication Date: 2022-06-03
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Application Information

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Problems solved by technology

Phenolic acids, such as organic acids, flavonoids and other important active ingredients in traditional Chinese medicine, are usually analyzed by reversed-phase chromatography, but due to the complexity of the ingredients, there are usually problems of insufficient separation selectivity and limited peak capacity. Combined with special separation materials, it provides The separation selectivity is different from that of reversed-phase materials, and the construction of an orthogonal two-dimensional separation system is expected to provide a new means for the separation of complex phenolic acids

Method used

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  • Separation method of phenolic acid components in traditional Chinese medicine
  • Separation method of phenolic acid components in traditional Chinese medicine
  • Separation method of phenolic acid components in traditional Chinese medicine

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Embodiment 1

[0030] The zwitterionic stationary phase bonded by pyrazine dianhydride was used to analyze ginkgo flavonoids. figure 1 , under the same mobile phase conditions, the zwitterionic stationary phase (ZAC) in the present invention has the longest retention time and good peak shape.

[0031] Chromatographic conditions:

[0032] Chromatographic column: PTZ (4.6*150mm, 3μm); XBridge Amide (4.6*150mm, 3.5μm);

[0033] ZIC HILIC(2.1*150mm,3.5μm); RZ5-ZACE(4.6*150mm,5μm)

[0034] Mobile phase: A-acetonitrile; B-water (containing 60 mM ammonium formate, pH 3.2);

[0035] Isocratic: A / B (82:18)

[0036] Flow rate: 1.0mL / min&0.2mL / min

[0037] Detection wavelength: 254nm

[0038] Sample preparation: Weigh out quercetin-3-O-β-D-glucoside, quercetin 3-O-β-D-(6”-p-coumaroyl)glucopyranosyl(1-2)-α-L-rhamnopyranoside, Rutin and quercetin -3-O-2", 6"-rhamnosyl glucoside appropriate amount, use 50% methanol / 50% water (v / v) to prepare a mixed sample solution with a concentration of 1 mg / mL, r...

Embodiment 2

[0040] Using the zwitterionic stationary phase bonded with the above pyrazine dianhydride to analyze organic acid compounds, the peak shape and retention of different chromatographic columns are quite different, such as figure 2 As shown in the figure, the ZAC chromatographic column has the best effect, which is mainly reflected in the longest retention time and the most symmetrical peak shape. When other chromatographic columns are used for analysis, there is a phenomenon of peak shape tailing.

[0041] The Lonicera japonica extract was analyzed using this chromatographic column, which is significantly different from the C18 chromatographic column analysis, such as image 3 As shown, the peak order is different from that of the C18 material, and the ZAC material shows better potential in the analysis of organic acids.

[0042] Analysis conditions of organic acid reference substance:

[0043] Chromatographic column: PTZ (4.6*150mm, 3μm); XBridge Amide (4.6*150mm, 3.5μm);

...

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Abstract

The invention discloses a method for separating phenolic acid components in traditional Chinese medicines, belongs to the field of analytical chemistry, relates to a method for separating phenolic acid components in traditional Chinese medicine samples, and in particular relates to a method for analyzing organic acid effective components in ginkgetin and honeysuckle based on a binary anhydride modified amino zwitterionic hydrophilic chromatography stationary phase. According to the method, a material shown in a structural diagram is adopted, mobile phase conditions are optimized, and an analysis method for flavonoids and organic acids in traditional Chinese medicines is established. The established method has different separation selectivity from a common reversed-phase C18 chromatographic column and other hydrophilic chromatographic columns, and is suitable for orthogonal separation of a traditional Chinese medicine complex system.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, in particular to a method for separating and analyzing phenolic acid components in traditional Chinese medicines by high performance liquid chromatography. technical background [0002] Hydrophilic Interaction Liquid Chromatography (HILIC) is a new chromatographic separation technique developed in recent years for the separation of highly polar compounds, first proposed by Alpert in 1990 ]. Similar to normal phase chromatography (NPLC), HILIC uses a polar stationary phase and a relatively less polar water / organic solvent as the mobile phase, where water is the strong elution solvent. HILIC can use a solution system with a large water content as the mobile phase, which can solve the problems that the mobile phase of normal phase chromatography has poor solubility relative to water-soluble substances, the retention time is very sensitive to the water content of the mobile phase, and is compatib...

Claims

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Application Information

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IPC IPC(8): B01D15/30B01J20/286B01J20/30
CPCB01D15/305B01J20/286
Inventor 梁鑫淼金高娃郭志谋丁俊杰
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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