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Application of bacillus cereus FNXJ1-2-3 in removal of hexavalent chromium in wastewater

A Bacillus cereus, hexavalent chromium technology, applied in the field of microorganisms, can solve the problems of unproven tolerance and resistance, and achieve the effect of strong adsorption and reduction

Pending Publication Date: 2022-06-03
YANCHENG INST OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, Bacillus cereus FNXJ1-2-3, which has similar genome information, can theoretically be used to deal with Cr(VI) pollution or Cr(VI) and Ni(II) co-contamination, but how is this strain applied to remove Cr(VI) and Ni(II) in practice? Its biosorption, tolerance and resistance have not yet been proven

Method used

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  • Application of bacillus cereus FNXJ1-2-3 in removal of hexavalent chromium in wastewater
  • Application of bacillus cereus FNXJ1-2-3 in removal of hexavalent chromium in wastewater
  • Application of bacillus cereus FNXJ1-2-3 in removal of hexavalent chromium in wastewater

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1 Biosorption-reduction test of Bacillus cereus on Cr(VI)

[0028] 1. Preparation of bacterial suspension

[0029] Take some LB culture medium of Bacillus cereus in logarithmic phase, centrifuge at 4°C and 13000r / min for 10min, add sterile water and repeat the centrifugation 3 times to prepare lyophilized powder. Before the test, take an appropriate amount of lyophilized powder and disperse it in sterile physiological saline, and the prepared bacterial content is 9.0*10^10 / mL bacterial suspension, which is stored at 4°C for future use.

[0030] 2. Single factor test of biosorption-reduction test of Bacillus cereus FNXJ1-2-3

[0031] Take 0.1~1mL of FNXJ1-2-3 bacterial suspension respectively, add it to 100mL of LB medium containing initial Cr(VI) concentration of 0~60mg / L, at pH3.0~pH8.0, adsorption temperature 28~37℃ , and cultured at 160r / min for 0-32h, to explore the single factor suitable conditions for FNXJ1-2-3 strain to remove Cr(VI) pollutants in Cr(VI...

Embodiment 2

[0042] Example 2 Tolerance and resistance characteristics of Bacillus cereus to Cr(VI)

[0043] Cr(VI) with concentrations of 0 mg / L, 10 mg / L, 20 mg / L, 30 mg / L, 40 mg / L, 50 mg / LL and 60 mg / L was added to 100 mL of LB medium, and the most The optimal log-phase Bacillus cereus culture solution, set 3 parallels for each concentration, randomly sample at regular intervals, measure the bacterial biomass, residual Cr(VI) and total Cr in the solution, and establish the bacterial culture of Bacillus cereus respectively. The time relationship curve of biomass, Cr(VI) removal rate and total Cr removal rate, such as Figure 7 shown.

[0044] Depend on Figure 7 It can be seen that with the increase of the initial Cr(VI) concentration, the bacteriostasis of microorganisms firstly rises rapidly and then tends to be flat, indicating that Cr(VI) has a significant inhibitory effect on the growth of microorganisms. The inoculum has a certain growth, so the strain has good adsorption, tolera...

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Abstract

The invention discloses application of bacillus cereus FNXJ1-2-3 in removal of hexavalent chromium in wastewater, and belongs to the technical field of microorganisms. Through a single factor test and a tolerance test, proper biological adsorption conditions of the strain on Cr (VI) wastewater are determined as follows: the initial Cr (VI) concentration is not more than 30 mg / L, the initial bacterium content is 0.9 * 10 < 8 > / mL to 4.5 * 10 < 8 > / mL, the initial pH is 7.0-pH 8.0, the adsorption time is 28-32 hours, the adsorption temperature is 34 DEG C, and the rotating speed is 160 r / min. When the optimized adsorption condition is balanced, the removal rates of the FNXJ1-2-3 strain on Cr (VI) and total chromium in Cr (VI) wastewater respectively reach 77.2% and 64.2%. The bacillus cereus FNXJ1-2-3 viable bacteria have very strong adsorption-reduction property, tolerance and resistance to Cr (VI) in Cr (VI) wastewater, and the action ranges of the bacillus cereus FNXJ1-2-3 viable bacteria are as follows: the initial Cr (VI) concentration is 0-30 mg / L, the initial Cr (VI) concentration is 40-50 mg / L, and the initial Cr (VI) concentration is greater than or equal to 60 mg / L.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to the application of Bacillus cereus FNXJ1-2-3 for removing hexavalent chromium in wastewater. Background technique [0002] Heavy metal compounds are widely used in industries such as metalworking, electroplating, electronics, mining operations, fertilizer production, battery manufacturing, and tanneries, and are responsible for direct or indirect emissions of heavy metals into the environment. Wastewater (waste) polluted by heavy metals (Cr, Cu, Ni, Co, Pb, Cd, Hg, Ag) and metalloids (As) and electroplating sludge rich in heavy metals have persistent biological toxicity and are harmful to humans. It has caused serious harm to health and the environment, which has caused great concern. Among them, Cr usually has two oxidation states, Cr(VI) (Cr(VI)) and trivalent chromium (Cr(III)). In living organisms, Cr (VI) generally has higher chemical toxicity than Cr(III...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F101/22
CPCC02F3/34C02F2101/22Y02W10/10
Inventor 李朝霞顾乃鹏仇树梅孙国凤丁成向明灯殷浩源徐静仪刘长青
Owner YANCHENG INST OF TECH