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Solid culture medium of coriolus versicolor as well as preparation method and application of solid culture medium

A solid medium, the technology of Yunzhi versicolor, which is applied in microorganism-based methods, biochemical equipment and methods, and adding compounds to stimulate growth, etc., can solve the problems of long cultivation time and easy contamination of Yunzhi versicolor. , to solve the problem of easy contamination and reduce costs

Active Publication Date: 2022-06-07
BEIJING UNIV OF CIVIL ENG & ARCHITECTURE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of this, the purpose of the present invention is to provide a solid culture medium for versicolor versicolor and its preparation method and application, so as to solve the problems that the traditionally adopted PDA medium for cultivating versicolor versicolor is long in cultivation time and easy to be infected with bacteria during the cultivation process , reduce cost, and increase the biomass and anti-staining ability of versicolor versicolor

Method used

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  • Solid culture medium of coriolus versicolor as well as preparation method and application of solid culture medium
  • Solid culture medium of coriolus versicolor as well as preparation method and application of solid culture medium
  • Solid culture medium of coriolus versicolor as well as preparation method and application of solid culture medium

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preparation example Construction

[0028] The present invention also provides a method for preparing the above-mentioned solid culture medium. When preparing each L of the solid culture medium, the following steps are included: mixing the bran with 500 mL of boiled water and then boiling for 25 minutes, and collecting the bran extract by filtration;

[0029] The bran extract is mixed with xylose, agar, trace element liquid and part of water, boiled, and the volume is adjusted to 1 L with water, and the solid medium is obtained by cooling after sterilization.

[0030] The filtration of the present invention preferably includes 9 layers of gauze.

[0031] The sterilization in the present invention preferably includes high temperature and high pressure sterilization, and more preferably autoclave sterilization at 121°C for 20 minutes. In the sterilization, the present invention preferably also includes evenly distributing the sterilized medium to each plate or petri dish. In the embodiment, a 90mm petri dish is sele...

Embodiment 1

[0038] The preparation of embodiment 1 solid medium

[0039] A. Preparation of trace element liquid, weigh 3.0g MgSO on the balance 4 ·7H 2 O, 0.5g MnSO 4 , 1.0g NaCl, 0.1g FeSO 4 ·7H 2 O, 0.1 g CoSO 4 , 0.082g CaCl 2 , 0.1g ZnSO 4 , 0.01g CuSO 4 ·5H 2 O, 0.01g KAl (SO 4 ) 2 , 0.01g H 3 BO 3 , 0.01g NaMoO 4 , dissolve in distilled water and dilute to 1000mL;

[0040] B, the balance weighs 40g bran, 30g xylose is standby;

[0041] C, after 500mL distilled water is boiled, add the bran taken by weighing, boil for 25min, filter with 9 layers of gauze to obtain bran leaching solution;

[0042] D. After adding distilled water appropriately, add 30g xylose, 1.5mL trace elements, agar with a final concentration of 2% (m / m), and boil;

[0043] E. After distilling the volume to 1000 mL with distilled water, autoclave at 121° C. for 20 min, pour 30 mL of culture medium into a 90 mm petri dish, and cool to obtain a solid medium for Yunzhi versicolor, which is used for lat...

Embodiment 2

[0044] Example 2 Inoculation of variegated Yunzhi

[0045] 1. Experimental example

[0046] A, on the sterile operating table, the PDA medium that is covered with variegated Yunzhi is made into the bacterial sheet of 10mm with aseptic puncher;

[0047] B, bacterial flakes are inoculated on the solid medium prepared in Example 1, and each medium is inoculated with 2 bacterial flakes;

[0048] C, utilize the sealing film to seal the lower part of the culture dish;

[0049] D. Place the inoculated solid medium in a biological incubator, and set the temperature to 28°C.

[0050] 2. Comparative ratio

[0051] Weigh 46 g of PDA (Potato Dextrose Agar, Qingdao Hi-Tech Industrial Park Haibo Biotechnology Co., Ltd.) to prepare a solid medium, and other operations are the same as in the experimental example, and 4 groups of repetitions are set for each.

[0052] The biomass was measured after 7 days in the biochemical incubator and the results were as follows figure 1 As shown, the ...

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Abstract

The invention provides a solid culture medium of coriolus versicolor as well as a preparation method and application thereof, and relates to the technical field of culture materials for fungi. According to the solid culture medium, the bran is adopted as a main component in the solid culture medium, the solid culture medium has the advantages of being economical, green, high in coriolus versicolor growth speed and high in microbiological contamination resistance, the problem that an existing culture medium is prone to microbiological contamination in the experiment process is solved, and the cost can be reduced. By utilizing the solid culture medium disclosed by the invention, biomass higher than that of a PDA (potato dextrose agar) culture medium and higher anti-bacterial performance can be obtained.

Description

technical field [0001] The invention belongs to the technical field of culture material for bacteria, and in particular relates to a solid culture medium of Yunzhi versicolor and a preparation method and application thereof. Background technique [0002] Coriolus versicolor, classified and identified from the northeastern forest area, is a typical white-rot fungus. Laccase is a biological enzyme known to degrade polycyclic aromatic hydrocarbons, petroleum hydrocarbons and halogenated hydrocarbons and other pollutants widely existing in the environment. , is a potential strain for soil remediation in the future. [0003] As the first step of soil bioremediation, it is necessary to carry out solid culture of the bacteria used for the restoration in the laboratory. However, the traditional potato dextrose agar (PDA) medium often has high costs and is prone to contamination during the experiment. The problem is that it cannot meet the requirement of obtaining repair strains in...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/38C12R1/645
CPCC12N1/14C12N1/38Y02P60/87C12N2500/10C12N2500/76
Inventor 高大文唐腾梁红王立涛
Owner BEIJING UNIV OF CIVIL ENG & ARCHITECTURE
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