Application of arabidopsis transcription factor SRG1 gene in regulation and control of plant growth and development
A transcription factor and plant growth technology, applied in the field of genetic engineering, can solve the problems of plant size regulation that have not been reported
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Embodiment 1
[0017] Example 1 Sterile culture and hypocotyl development of Arabidopsis thaliana
[0018] Seeds of Arabidopsis Col-0, srg1 and SRG-OX were taken, respectively, the seeds were placed in a 1.5 ml centrifuge tube, sterilized with 70% ethanol for 1 min, followed by 1% sodium hypochlorite (effective chlorine concentration of 5%) for 5-8 min, and finally washed four times with sterile water. Spread the seeds evenly on 1 / 2 MS solid medium.
[0019] After 2 days of dark treatment at 4 °C, the seeds were moved to a plant incubator, plants were cultured upright to 6 days of age under 10 h light: 16 h dark photoperiod conditions, followed by photographs of plant growth and measurement of plant root length ( Figure 1 )。
Embodiment 2
[0020] Example 2 Arabidopsis leaf size and number analysis
[0021] Seeds of Arabidopsis Col-0, srg1 and SRG-OX were sown to MS medium according to the sterile culture method in Example 1, treated in the dark at 4 °C for 2 days, and then placed in a plant incubator for horizontal culture. Plants are transplanted in soil (grass charcoal: vermiculite = 3:2) after 10 days in the incubator, and the plants are cultured under the same photoperiod conditions for the next experiment.
[0022] When the plant is 4 weeks old, take a picture of the fully extended rosette leaves of the plant and measure the leaf surface area at 6 weeks ( Figure 2 and Figure 3 )。 At the same time, the number of leaves of plants aged 4, 5 and 6 weeks was recorded respectively ( Figure 4 )。
Embodiment 3
[0023] Example 3 Arabidopsis plant type observation
[0024]Seeds of Arabidopsis Col-0, srg1 and SRG-OX were sown to MS medium according to the sterile culture method in Example 1, treated in the dark at 4 °C for 2 days, and then placed in a plant incubator for horizontal culture. After the plants were cultured in the incubator for 10 days, the plants were transplanted in the soil (grass charcoal: vermiculite = 3:2), and the plants were cultivated to 7 weeks later, the plant height and plant type were photographed and observed ( Figure 5 )。
[0025] The above provided embodiment is the arabidopsis transcription factor SRG1 gene applied to plant leaf size, hypocotyl development and plant size research.
[0026] Although, the present invention has been described in detail with a general description and specific embodiments above, but on the basis of the present invention, it may be slightly modified or improved, which is obvious to those skilled in the art. Similarly, the constructi...
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