High-glucose-resistant lactic acid production strain and application thereof in D-lactic acid production

A technology of lactic acid and lactate dehydrogenase, applied in the field of microorganisms, can solve problems such as increasing production costs

Active Publication Date: 2022-06-21
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the bacterium has the advantages of high fermentation temperature, high conversion rate of sugar and acid, and high product purity, the growth of the bacterium is inhibited by high concentration of glucose, and a large amount of yeast powder needs to be added during the fermentation process, which increases the production cost.

Method used

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  • High-glucose-resistant lactic acid production strain and application thereof in D-lactic acid production
  • High-glucose-resistant lactic acid production strain and application thereof in D-lactic acid production
  • High-glucose-resistant lactic acid production strain and application thereof in D-lactic acid production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, the acquisition of Bacillus coagulans (Bacillus coagulans) G35 and G35-LjLDH and identification of fermentation characteristics

[0054] In this example, Bacillus coagulans (Bacillus coagulans) D-DSM1 was used as the starting strain, and the atmospheric pressure room temperature plasma (ARTP) mutagenesis technology was used in combination with the adaptive evolution technology to screen and obtain a strain that can tolerate high concentrations of initial sugar (200 gram per liter of initial sugar), in low yeast (less than 1 gram per liter) culture medium producing D-lactic acid strain G35, its D-lactic acid production can reach 134 grams per liter of fermentation broth. details as follows:

[0055] 1. ARTP mutagenesis

[0056] 1.1 Mutagenesis process

[0057] Bacillus coagulans (Bacillus coagulans) D-DSM1 was activated with 513 liquid medium (containing glucose 50g / L, yeast powder 10g / L and neutralizing agent 30g / L) for 12 hours, and streaked 513 solid m...

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Abstract

The invention discloses a high-glucose-resistant lactic acid production strain and application thereof in D-lactic acid production. The invention provides bacillus coagulans G35, and the preservation number of the bacillus coagulans G35 in the China General Microbiological Culture Collection Center is CGMCC (China General Microbiological Culture Collection Center) No. 21293. The invention further provides the bacillus coagulans G35-LjLDH, and the bacillus coagulans G35-LjLDH is a recombinant strain obtained by replacing a D-lactic dehydrogenase gene of a strain G35 with a D-lactic dehydrogenase gene from lactobacillus jensenii. The strain developed and obtained by the invention has the characteristics of high glucose tolerance, capability of utilizing a low-concentration organic nitrogen source, high temperature resistance and high D-lactic acid yield, and has important practical significance.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a high-sugar-resistant lactic acid production strain and its application in D-lactic acid production. Background technique [0002] Polylactic acid is a biodegradable polymer produced from optically pure lactic acid (optical purity greater than 99%). Polylactic acid has good plasticity and can be processed by the same process as general-purpose thermoplastics to be made into disposable tableware, packaging materials, orthopedic internal fixation materials, non-disassembly surgical sutures and other products, and has a very broad market prospect. Polylactic acid also has good biodegradability, can be absorbed by microorganisms in seawater, and can also be completely degraded into carbon dioxide and water under compost or soil conditions. With the enhancement of people's awareness of environmental protection, polylactic acid, as a new type of environmentally friendly polymer material...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21C12N15/90C12N15/53C12P7/56C12R1/07
CPCC12N9/0006C12N1/20C12N15/902C12P7/56C12Y101/01028Y02E50/10
Inventor 王丽敏张正芝佘佳佳于波
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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