Mutant salicylic acid decarboxylase, strain and application of mutant salicylic acid decarboxylase in ginkgolic acid degradation

Abstract

The invention discloses a mutant salicylic acid decarboxylase capable of efficiently degrading ginkgolic acid. The mutant salicylic acid decarboxylase is a mutant enzyme Sdc-P191A and / or a mutant enzyme Sdc-Y64. The mutant enzyme Sdc-P191A is characterized in that the 191st amino acid of salicylic acid decarboxylase sdc with the amino acid sequence of SEQ ID NO.1 is changed from proline to alanine, and the amino acid sequence of the mutant enzyme Sdc-P191A is shown as SEQ ID NO.2; the amino acid sequence of the mutant enzyme Sdc-Y64T is as follows: the 64th amino acid of salicylic acid decarboxylase sdc of SEQ ID NO.1 is changed from tyrosine to threonine, and the amino acid sequence of the mutant enzyme Sdc-Y64T is as shown in SEQ ID NO.3. According to the invention, part of amino acids are selected for site-directed mutagenesis, two mutant salicylic acid decarboxylases are successfully constructed, and two mutant enzymes capable of efficiently degrading ginkgolic acid compared with wild sdc are obtained.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, in particular to the application of mutant salicylic acid decarboxylases Sdc-P191A and Sdc-Y64T in the degradation of ginkgolic acid. Background technique [0002] Ginkgo is not only a kind of traditional Chinese medicine, which contains a variety of active ingredients. It is usually used to relieve cough, moisten the lungs, eliminate phlegm, treat turbidity and reduce stool, etc. It is also used for the treatment of tuberculosis; it is also a health food, which can maintain health. It was once praised as a royal tribute and loved by people. [0003] In modern research, it is found that ginkgo contains more linoleic acid, which can improve hyperlipidemia and hypertension to a certain extent, and can prevent cardiovascular diseases, and can also kill cancer cells to a certain extent. culture; some studies have found that the presence of vitamin E in ginkgo can strengthen the body's ab...

AI Technical Summary

Problems solved by technology

[0004] There have been many reports on the extraction and determination methods of ginkgolic acid. Decarboxylation and degradation of ginkgolic acid is one of the important means, and existing biological research methods have shown that salicylic acid decarboxylase (Sdc) from Trichosporium can decarboxylate and degrade ginkgolic acid. Function, can decarboxylate ginkgolic acid to non-toxic products, but previous studies have shown that wild-type salicylate decarboxylase has low performance in degrading ginkgolic acid

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