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Molecular marker-assisted breeding method of large yellow croaker capable of resisting visceral white-spot disease

A molecular marker-assisted, visceral white spot disease technology, applied in biochemical equipment and methods, proteomics, microbial determination/inspection, etc., to reduce the cost of breeding and speed up the cycle.

Pending Publication Date: 2022-06-24
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the molecular marker-assisted breeding of large yellow croaker's resistance to visceral white spot disease.

Method used

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  • Molecular marker-assisted breeding method of large yellow croaker capable of resisting visceral white-spot disease
  • Molecular marker-assisted breeding method of large yellow croaker capable of resisting visceral white-spot disease
  • Molecular marker-assisted breeding method of large yellow croaker capable of resisting visceral white-spot disease

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Experimental program
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Effect test

Embodiment 1

[0017] Example 1 Breeding of large yellow croaker population against visceral white spot disease

[0018] Experimental Materials:

[0019] The test material was large yellow croaker, which was raised in Fujian Ningde Jinling Aquatic Technology Co., Ltd. Pseudomonas mutans strains are commercially available (eg CS-0019-1315 from Gade Chemical Network).

[0020] 1. Determination of the phenotypic value of the resistance to visceral white spot disease in the reference population:

[0021] The reference group (800 large yellow croaker) was challenged by the immersion challenge method. After the water level in the culture pond was lowered, the Pseudomonas mutans bacteria solution was sprinkled (to the concentration of the bacteria solution in the pool was 10 6 cfu / mL), the cell remains aerated. Soak all the large yellow croakers in the pool for 3 hours and then take them out. After washing with clean sea water, put them into the clean sea water pool. Check the fish status every ...

Embodiment 2

[0030] Experimental materials: The experimental data is the anti-visceral white spot disease-resistant breeding population obtained by molecular marker-assisted breeding of anti-visceral white spot disease. The cultivated fry are cultured in the fishing rafts of the demonstration site of Ningde Jingli Aquatic Products Co., Ltd. and the Ningbo Comprehensive Experimental Station Xiangshan Bay cage breeding base.

[0031] 1. Comparison of the survival rate of the anti-visceral white spot breeding population and the normal control group

[0032] When the visceral white spot disease broke out in the sea area where the fishing rafts of Ningde Jingli Aquatic Products Co., Ltd. were located in the summer of 2019, the large yellow croaker in the control group continued to die, while the disease-resistant breeding group rarely died.

[0033] 30 tails in the control group and the disease-resistant breeding group were selected for anatomical observation and the content of Pseudomonas muta...

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Abstract

The invention discloses a molecular marker-assisted breeding method of large yellow croaker capable of resisting visceral white-spot disease. The method comprises the following steps: firstly, determining an individual disease resistance phenotypic value of a reference group through an artificial challenge experiment; performing genome re-sequencing on the extreme phenotype individuals to obtain SNP sites; screening out qualified SNP (Single Nucleotide Polymorphism) sites, and filling the deleted genotypes; the significance of correlation between each SNP site and a phenotypic value is calculated through GWAS, and the most significant first 5-30 SNPs are selected as molecular markers for assistant breeding. The method comprises the following steps: carrying out individual marker identification on parent fish of a candidate group, carrying out genetic typing by virtue of MassArray or other SNP typing methods, calculating a genome estimation breeding value by virtue of a GBLUP method, selecting the parent fish according to the breeding value, and culturing the large yellow croaker capable of resisting the visceral white-spot disease. According to the method, the breeding effect can be greatly improved, large yellow croaker groups with effectively improved disease resistance can be rapidly bred, the breeding period is shortened, and the cost is reduced.

Description

technical field [0001] The invention relates to the field of aquatic species breeding, in particular to a molecular marker-assisted breeding method for large yellow croaker against visceral white spot disease. Background technique [0002] Large yellow croaker is the largest marine fish in my country, but due to factors such as the increase in breeding density, the degradation of germplasm, and the deterioration of the aquaculture water environment, various diseases frequently occur during the breeding process of large yellow croaker. Among them, large yellow croaker is the most popular. Visceral white spot disease. The visceral white spot disease of large yellow croaker is a bacterial disease caused by Pseudomonas proteus. The disease is a disease in the body, and symptoms are difficult to find in the early stage of the disease. In the later stage of the disease, the liver, spleen and kidney of the diseased fish are covered with white bacterial nodules. , the drug treatment...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888G16B20/20G16B20/30
CPCC12Q1/6888G16B20/20G16B20/30C12Q2600/156C12Q2600/124
Inventor 王志勇李泽宇李完波方铭
Owner JIMEI UNIV
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