Content detection method for flavor characteristic components of rhizoma kaempferiae
A detection method and technology of sage, applied in the direction of color/spectral property measurement, measurement device, analysis material, etc., can solve the problems of difficulty in accurately reflecting the flavor of sage, and difficulty in comprehensively reflecting the flavor of sage.
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Embodiment 1
[0068] The present embodiment is the construction of the content detection method of the flavor characteristic components of sand ginger.
[0069] (1) Construction of standard curve of ethyl p-methoxycinnamate
[0070] Accurately weigh 4.90 mg of ethyl p-methoxycinnamate in a beaker, add a small amount of absolute ethanol to dissolve, and dilute to a 100-mL volumetric flask with absolute ethanol, and then take 1 mL, 2 mL, 3 mL, 4 mL, and 5 mL of the above-determined volume. The prepared solution was placed in a 25mL test tube with a stopper, and anhydrous ethanol was added until the solution volume was 25mL, and the concentrations were respectively 1.96ug / mL, 3.92ug / mL, 5.88ug / mL, 7.84ug / mL, and 9.80ug / mL. Measure the absorbance value at 309 nm with absolute ethanol as blank.
[0071] The results are shown in Table 1 below, take the absorbance as the abscissa, the ethyl p-methoxycinnamate concentration as the ordinate, draw a standard curve, and the gained p-methoxycinnamate ...
Embodiment 2
[0109] The present embodiment is the content detection of the flavor characteristic components of Guangxi sand ginger.
[0110] Accurately weigh 1.000g of Guangxi sand ginger powder into a 100mL glass bottle with a lid, add 70.00g of absolute ethanol, maintain the water bath to 80°C, and use an ultrasonic power of 800W for ultrasonic extraction for 30min. After the extraction, the solution and the slag were separated, and the slag was extracted once again according to the same solid-liquid ratio and extraction conditions, for a total of 2 extractions. The extracts extracted twice by ultrasonic wave were combined, shaken, and then all transferred to filter paper folded into a funnel for filtration, and the filtrate was collected to obtain the Guangxi Shajiang extract.
[0111] Accurately weigh 0.200g of Guangxi Shajiang extract in a 25mL test tube with a stopper, add 20.000g of absolute ethanol to determine the content of ethyl p-methoxycinnamate, name it as sample A, and measu...
Embodiment 3
[0123] This embodiment is the content detection of the flavor characteristic components of Thai sand ginger.
[0124] Weigh 1.000g of Thai sand ginger powder into a 100mL glass bottle with a lid, add 70.00g of absolute ethanol, maintain the water bath to 80°C, and use an ultrasonic power of 800W for ultrasonic extraction for 30min. After the extraction, the solution and the slag were separated, and the slag was extracted once again according to the same solid-liquid ratio and extraction conditions, for a total of 2 extractions. The extracts extracted twice by ultrasonic wave were combined, shaken, and then all transferred to filter paper folded into a funnel for filtration, and the filtrate was collected to obtain the Thai ginger extract.
[0125] Accurately weigh 0.200g of Thai ginger extract in a 25mL test tube with a stopper, add 20.000g of absolute ethanol to determine the content of ethyl p-methoxycinnamate, name it as sample A, and measure the absorbance of sample A at 3...
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