Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Microbial complex microbial inoculant and application thereof in prevention and treatment of plant diseases

A technology of microbial compound bacteria and compound bacteria agent, applied in the field of microorganisms, can solve the problems of resistance of pathogenic microorganisms, less effective microbial resources, environmental degradation, soil-borne diseases, etc., to improve yield and quality, excellent antagonistic effect, increase The effect of bacteriostatic ability

Pending Publication Date: 2022-06-28
SHANDONG QINGDAO TOBACCO +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of chemical pesticides to control tobacco bacterial wilt and tobacco black shank has been the fastest and most effective way in recent decades, but this control method not only causes the flooding of chemical pesticides, but also easily leads to the development of resistance of pathogenic microorganisms, resulting in Vicious cycle of environmental degradation and soil-borne diseases becoming more and more serious
[0003] Based on the above-mentioned status quo, microbial agents currently used as chemical pesticide substitutes have the advantages of being safe to use, pollution-free, and pathogenic microorganisms are not easy to develop resistance, and have good application prospects; however, at present, those with excellent control effects on plant diseases There are few effective microbial resources, and there are deficiencies such as narrow application range and slow effect
And, the control effect of existing individual microorganism is limited, it is difficult to obtain further improvement

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbial complex microbial inoculant and application thereof in prevention and treatment of plant diseases
  • Microbial complex microbial inoculant and application thereof in prevention and treatment of plant diseases
  • Microbial complex microbial inoculant and application thereof in prevention and treatment of plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Strain compatibility detection compound primary screening

[0039] 1. Experimental method:

[0040] A number of strains of Bacillus amyloliquefaciens F012, F028, L1, F01, F040, L44, and L36 were screened out in the early stage of the laboratory, which have highly effective antagonistic effects on tobacco soil-borne diseases. The strains were activated and cultured in pairs, including: F028-F012, F028-L1, F012-L1, F028-F01, F012-F01, F012-F040, F028-F040, F028-L36. The method of streaking culture is as follows: use an inoculation loop to take the activated corresponding bacterial solutions and streak them on a nutrient agar plate in a tic-tac-toe manner, and cultivate at a constant temperature of 30 °C for 24 hours. sex.

[0041] 2. Experimental results and analysis:

[0042] Depend on figure 1 It can be seen that in the confrontation culture of multiple strains, only F012 and F028 are compatible, and all other combinations all have bacterial antagonism. T...

Embodiment 2

[0043] Embodiment 2 Bacillus amyloliquefaciens F012, F028 strain compatibility detection rescreening

[0044] 1. Experimental method:

[0045] Method 1: Use an inoculating loop to take the activated bacterial solutions of F012 and F028, and scribble them on a nutrient agar plate in a tic-tac-toe manner, incubate at 30°C for 24 hours, and observe the colony growth at the colony junction node.

[0046] Method 2: firstly culture Bacillus amyloliquefaciens F012 and F028 in shake flasks, activate and multiply, prepare NA solid medium, after the medium is sterilized, reduce the temperature to about 55 ℃, add 1 ml of activated Bacillus amyloliquefaciens F012 , pour the plate, and incubate at 28°C for 24h after solidification. Bacillus amyloliquefaciens F028 is poured as above-mentioned steps, and after solidification, use a punch to cultivate to the carrier medium (mixture of culture medium and bacteria) that is full of Bacillus amyloliquefaciens F012 and the uncultivated with amylo...

Embodiment 3

[0050] Embodiment 3 Bacillus amyloliquefaciens F012, F028 compound mode

[0051] 1. Experimental method

[0052] In order to study the best culture mode of Bacillus amyloliquefaciens F012, F028, following three kinds of culture methods were designed, respectively obtained four kinds of bacterial liquids, used for follow-up experiments:

[0053] A. Mixed fermentation bacteria liquid:

[0054] Bacillus amyloliquefaciens F012 and F028 were cultured in shake flasks for 12h, and after activation and propagation, 1ml of bacterial liquid was taken (diluted to OD with sterile water, respectively). 600 =1) In 100 mL of LB medium, culture at 28°C for 36 h to obtain a mixed fermented bacterial liquid.

[0055] B. Fermentation mixed bacterial liquid:

[0056] Bacillus amyloliquefaciens F012 and F028 were cultured in shake flasks for 12h respectively, activated and propagated, and 1ml of bacterial liquid (respectively diluted to OD) was obtained. 600 =1) Incubate in 100 mL LB medium at...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a microbial complex microbial inoculant and an application of the microbial complex microbial inoculant in prevention and treatment of plant diseases. The microbial complex microbial inoculant comprises bacillus amyloliquefaciens F028 and bacillus amyloliquefaciens F012, and the microbial complex microbial inoculant is prepared from bacillus amyloliquefaciens F028, bacillus amyloliquefaciens F012, bacillus amyloliquefaciens F012, bacillus amyloliquefaciens F013, bacillus amyloliquefaciens F013, bacillus amyloliquefaciens F013, bacillus amyloliquefaciens F013, bacillus amyloliquefaciens F013, bacillus amyloliquefaciens F013, bacillus amyloliquefaciens The preservation number of the bacillus amyloliquefaciens F028 is CGMCC (China General Microbiological Culture Collection Center) No.19590 The preservation number of the bacillus amyloliquefaciens F012 is CGMCC (China General Microbiological Culture Collection Center) The compound microbial inoculant not only has excellent broad-spectrum antibacterial activity, but also has significantly improved disease control ability compared with a single strain, and also has an obvious growth promoting effect on plants. The field application of the compound microbial inoculant not only can effectively prevent and treat various plant diseases, but also can greatly improve the yield and quality of plants, and has very high application value.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a microbial compound inoculant and its application in preventing and treating plant diseases. Background technique [0002] At present, soil-borne diseases have become the second largest disease restricting tobacco yield and quality. Tobacco soil-borne diseases mainly include tobacco bacterial wilt and tobacco black shank. The application of chemical pesticides to control tobacco bacterial wilt and tobacco black shank is the most efficient and effective way in recent decades, but this control method not only causes the flood of chemical pesticides, but also easily leads to the resistance of pathogenic microorganisms, resulting in A vicious circle of environmental degradation and increasingly serious soil-borne diseases. [0003] Based on the above status quo, the current microbial inoculum as a substitute for chemical pesticides has the advantages of being safe to use, no...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00A01P21/00C12R1/07
CPCC12N1/20A01N63/22
Inventor 刘新赵方贵刘知祥叶青刘广超李雅华王鹏王世建丁志勇王耀斌
Owner SHANDONG QINGDAO TOBACCO
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com