Brain natriuretic peptide detection device and detection method based on surface enhanced Raman scattering technology

A surface-enhanced Raman and detection device technology, applied in the field of analysis and detection, can solve problems such as weak signals, and achieve the effects of reduced detection time, short Raman detection time, high sensitivity and high selectivity detection

Pending Publication Date: 2022-06-28
ZHEJIANG UNIV OF TECH
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to the selection law of quantum mechanics, the scattering cross section of the Raman effect is only one ten-billionth of the fluorescence signal, and the signal is very weak

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Brain natriuretic peptide detection device and detection method based on surface enhanced Raman scattering technology
  • Brain natriuretic peptide detection device and detection method based on surface enhanced Raman scattering technology
  • Brain natriuretic peptide detection device and detection method based on surface enhanced Raman scattering technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation method of Au nanofinger / BNP antibody / Au nanofinger coupling structure includes the following steps:

[0036] Step 1: Au nano-finger ordered array structure is prepared by nano-imprinting, Au film deposition and reactive ion beam etching. The height of a single nano-finger is 350 nanometers, including 300 nanometers of polymer pillars and 50 nanometers of Au above, with a diameter of 350 nanometers. is 75 nanometers, and the distance between adjacent fingers is 110 nanometers;

[0037] Step 2: Take out the brain natriuretic peptide (BNP) antibody from the -20 ℃ freezer, after thawing at room temperature, use a 10ul pipette to measure 1ul of the antibody;

[0038] Step 3: drop the antibody in step 2 on the metal nanofinger array structure obtained in step 1 and incubate for 8 hours. During the incubation, it was stored in a refrigerator at 4°C, and volatilized under natural conditions to form an Au nanofinger closed array structure, specifically Au nanofi...

Embodiment 2

[0041] The high-selectivity and high-sensitivity quantitative detection of BNP molecules based on Au nanofinger / BNP antibody / Au nanofinger coupling structure includes the following steps:

[0042] Step 1: Drop the antibody and the concentration of 10 on two 25nm thick gold films respectively 4 The brain natriuretic peptide was detected by Raman and the characteristic peaks were demarcated. Specifically as image 3 shown. The peak of brain natriuretic peptide is at 2100cm -1 , 2200cm -1 place.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses a device and a method for detecting a serological marker brain natriuretic peptide secreted by acute myocardial infarction based on a surface enhanced Raman scattering technology. The preparation method comprises the following steps: firstly, modifying antibody molecules corresponding to BNP through nano-imprinting and chemical bonds to complete preparation of an SERS substrate chip; in the volatilization process of a BNP antibody solution, a collapse finger array structure taking a hexamer or a polymer as a structural unit is formed. According to the invention, high-selectivity and high-sensitivity SERS detection of BNP can be realized at the same time, and high-selectivity and high-sensitivity measurement of specific BNP in a liquid serum environment within 5 seconds can be realized by directly performing optical focusing on the surface of an Au finger by dropping serum into a coupled structure substrate which is placed into a biological carrier in advance.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a device and method for detecting brain natriuretic peptide, a secretion marker of acute myocardial infarction, based on surface-enhanced Raman scattering technology. Background technique [0002] The incidence of cardiovascular disease represented by Acute Myocardial Infarction (AMI) is increasing year by year. AMI has sudden onset, rapid disease evolution, and arrhythmia at any time, leading to sudden cardiac death. It has a high disability rate and is a cardiovascular emergency. At present, AMI has surpassed lung cancer as the main disease with the highest mortality rate. [0003] At present, the fastest clinical biochemical detection of AMI in the world is the detection of myoglobin and troponin, which usually takes 20 minutes. The "Troponin I Rapid Test Card" developed and produced by Shanghai Chuanzhi Biotechnology Co., Ltd. can realize the visu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/65G01N21/01
CPCG01N21/658G01N21/01G01N2021/0112
Inventor 刘凡新
Owner ZHEJIANG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap