Albumin nanoparticles coupled with indocyanine green and oxaliplatin as well as preparation and application of albumin nanoparticles
A technology of albumin nanoparticles and indocyanine green, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve problems such as unsatisfactory encapsulation efficiency, achieve efficient inhibition of tumor cell growth, significant anti-tumor synergy, Achieving prep-level effects
Pending Publication Date: 2022-07-12
SUZHOU UNIV
View PDF1 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Most of the methods of physical encapsulation are used to make anticancer drugs into drug-loaded nano-preparations, but because oxaliplatin is a water-soluble drug, the encapsulation efficiency is often not ideal
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0028] Take 916.5 mg of Intermediate A, add 3.9 mL of absolute ethanol, shake in a water bath at 20°C (20 Hz) for 2 min, and add 35.1 mL of water to obtain a suspension of Intermediate A.
[0029] To the obtained suspension of Intermediate A, 1 mL of silver nitrate aqueous solution (820 mg mL -1 , about 4.8 mmol), react in the dark for 1 h, filter, and collect the filtrate (about 40 mL). Take 0.5mL of filtrate and add 5% sodium chloride aqueous solution without precipitation, indicating that it does not contain free Cl - . The filtrate is an OXP intermediate solution (dihydrate cyclohexanediamine platinum ion, about 60 mM in terms of Pt), for use, and the reaction equation is shown as follows figure 1 .
[0030] Weigh 200 mg of BSA, add 10 mL of water to dissolve to obtain a BSA solution (20 mg mL -1 ), add 1 mL of the obtained OXP intermediate solution (about 0.06 mmol) to the BSA solution under normal stirring, and then adjust the pH to 6.0 with 2 M NaOH aqueous solution...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
| Property | Measurement | Unit |
|---|---|---|
| particle size | aaaaa | aaaaa |
| concentration | aaaaa | aaaaa |
| particle size | aaaaa | aaaaa |
Login to View More
Abstract
The invention discloses albumin nanoparticles coupled with indocyanine green and oxaliplatin as well as preparation and application of the albumin nanoparticles, and the albumin nanoparticles (OXP-ICG NPs) co-coupled with indocyanine green and oxaliplatin are successfully prepared by adopting a new process and a biomineralization method. The preparation conditions of the nanoparticles are mild, and the preparation method is simple and easy to implement. Through related characterization of pharmaceutics, the nanoparticles meet the requirements of a passive targeted drug delivery system. In cell tests, MTT tests and chemotherapy-phototherapy combined nanoparticles OXP-ICG NPs have the following remarkable effects on CT26 colon cancer cells: 1) the tumor inhibition effect (IC50 is reduced from 12.23-28.56 to 4.77); 2) an anti-tumor synergistic effect (the synergistic index is 0.56) is achieved, the OXP phagocytosis amount of tumor cells can be increased by 1.45 times, and the cell apoptosis can be increased by 94.33%, so that the OXP-ICG NPs is proved to efficiently inhibit the growth of the tumor cells and have the chemotherapy-phototherapy synergistic treatment effect, and the new preparation process improves the intermediate preparation level.
Description
technical field [0001] The invention belongs to the technology of drug preparation, and particularly relates to albumin nanoparticles coupled with indocyanine green and oxaliplatin, and preparation and application thereof. Background technique [0002] Currently, chemotherapy is still one of the most important treatments in the face of cancer, the enemy of health [Siegel RebeccaL,Miller Kimberly D,Fuchs Hannah E, et al. Cancer Statistics[J]. 2021 CACancer J Clin, 2021, 71: 7-33]. The third generation platinum antitumor drug oxaliplatin (OXP), which can treat colon cancer, rectal cancer, etc., is the product of the action of chloroplatinite and trans-cyclohexanediamine. After heating and suspending in water, it is mixed with silver nitrate. It is obtained from the effect of potassium oxalate and is a commonly used clinical drug. However, due to its high binding to plasma proteins, low bioavailability, and large toxic and side effects, its application is limited [RottenbergS...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/51A61K47/64A61P35/00B82Y5/00A61K31/555
CPCA61K31/555A61K41/0057A61K47/643B82Y5/00A61P35/00A61K9/5169
Inventor 杨红陈亮陈华兵邓益斌柯亨特李明董知妤吴优璇
Owner SUZHOU UNIV