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Fluorescent nano-hydroxyapatite as well as preparation method and application thereof

A hydroxyapatite, fluorescent nanotechnology, applied in nano-carbon, nanotechnology, nano-optics and other directions, can solve the problems of limited application, tissue and cell damage, etc., and achieve the effect of stable fluorescence, small size, and increased conjugated structure

Pending Publication Date: 2022-07-15
华南师大(清远)科技创新研究院有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the tissue cells of the organism are sensitive to this low-band blue fluorescence and are easily absorbed by the tissue cells, it is difficult for this low-band fluorescence to penetrate the cell tissue for tracking imaging, and it is easy to damage the tissue cells. Limiting its further application in the biomedical field

Method used

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  • Fluorescent nano-hydroxyapatite as well as preparation method and application thereof
  • Fluorescent nano-hydroxyapatite as well as preparation method and application thereof
  • Fluorescent nano-hydroxyapatite as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of carbon dots

[0038] (1) Take 8 g of citric acid and 2 g of o-phenylenediamine in a beaker, add 40 mL of dimethylformamide, and stir with a glass rod for 1 h until the solution is clear.

[0039] (2) The solution was transferred into a polytetrafluoroethylene-lined autoclave, and reacted at 200° C. for 6 hours.

[0040] (3) After the reaction kettle is naturally cooled, the product mixture is obtained, and the mixture is dialyzed for 24 hours using a dialysis bag with a molecular weight cut-off of 500 Da. The retentate after dialysis was lyophilized to obtain carbon dot powder.

[0041] figure 1 It is a transmission electron microscope (TEM) image of the carbon dots in Example 1 of the present invention.

[0042] figure 2 are the fluorescence spectra of the carbon dot solution in Example 1 of the present invention under different excitation wavelengths.

[0043] refer to figure 1 , the carbon dots obtained in Example 1 are spherical nanoparticles w...

Embodiment 2

[0046] Preparation of Nano-hydroxyapatite

[0047] (1) Measure 12.5 mL, 0.25 mol / L of calcium nitrate, and add it dropwise to 37.5 mL, 0.05 mol / L of sodium phosphate solution through a peristaltic pump at a speed of 0.5 mL / min.

[0048] (2) After all the dropwise additions, adjust the pH to 13 with NaOH solution (5 mol / L), stir the mixture at room temperature (25° C.) for 12 hours, and let it stand for 12 hours.

[0049] (3) After the reaction, the product mixture was transferred to a centrifuge tube, washed with water for 3 times and anhydrous ethanol for 3 times under the conditions of 10,000 rpm and 3 min, dried and ground to obtain nano-hydroxyapatite.

[0050] The nano-hydroxyapatite obtained in this example has no fluorescent properties.

Embodiment 3

[0052] Preparation of green fluorescent nano-hydroxyapatite

[0053] (1) Take 8 g of citric acid and 2 g of o-phenylenediamine in a beaker, add 40 mL of dimethylformamide, and stir with a glass rod for 1 h until the solution is clear.

[0054] (2) The solution was transferred into a polytetrafluoroethylene-lined autoclave, and reacted at 200° C. for 6 hours.

[0055](3) After the reaction kettle is naturally cooled, the product mixture is obtained, and the mixture is dialyzed for 24 hours using a dialysis bag with a molecular weight cut-off of 500 Da. The retentate after dialysis was lyophilized to obtain carbon dot powder.

[0056] (4) Weigh 0.05 g of the obtained carbon dot powder into a beaker, add 37.5 mL of 0.05 mol / L sodium phosphate solution, and stir magnetically for 1 h.

[0057] (5) Measure 12.5 mL, 0.25 mol / L of calcium nitrate, and add it dropwise to the mixed solution obtained in step (1) at a speed of 0.5 mL / min through a peristaltic pump.

[0058] (6) After a...

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Abstract

The invention discloses fluorescent nano-hydroxyapatite as well as a preparation method and application thereof. The fluorescent nano-hydroxyapatite is prepared by the following steps: dissolving citric acid and o-phenylenediamine in a solvent, reacting to obtain a product mixed solution, dialyzing the product mixed solution, and drying to obtain carbon dot powder; uniformly mixing the carbon dot powder with a phosphorus source solution, then dropwise adding a calcium source solution, adjusting the pH value, stirring at room temperature, standing to obtain a reaction product, washing and drying to obtain the green fluorescent nano-hydroxyapatite. And calcining, washing and drying the green fluorescent nano-hydroxyapatite, so as to obtain the orange fluorescent nano-hydroxyapatite. The preparation method has the advantages that the preparation process is simple, the used raw materials are low in price, non-toxic, environment-friendly and the like, and the prepared nano-hydroxyapatite particles are small in size, good in hydrophilicity and stable in fluorescence and have potential application prospects in the fields of luminescent materials, fluorescence tracing and nano-medicines.

Description

technical field [0001] The invention belongs to the field of inorganic biomedical materials, and particularly relates to a fluorescent nanometer hydroxyapatite and a preparation method and application thereof. Background technique [0002] Since the discovery of carbon dots in 2004, their excellent physical and chemical properties have attracted extensive attention of scholars. Carbon dots have low toxicity, good biocompatibility, and excellent photoluminescence properties. At present, carbon dots have shown great application potential in many fields such as fluorescent labeling, sensors, and catalysis. [0003] Hydroxyapatite is the main inorganic component of human and animal bones. It is widely used in the field of biomedicine due to its excellent biocompatibility, biological activity and non-toxicity. It can be used as bone tissue repair material, drug carrier, etc. [0004] In recent years, as research has found that nano-hydroxyapatite itself also has certain anti-ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B32/15C01B25/32B82Y30/00B82Y40/00B82Y20/00C09K11/65A61L27/12A61K47/02
CPCC01B32/15C01B25/325C01B25/327B82Y30/00B82Y40/00B82Y20/00C09K11/65A61L27/12A61K47/02C01P2004/80C01P2006/60
Inventor 邓春林王论珠任俭王心如
Owner 华南师大(清远)科技创新研究院有限公司
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