Supercharge Your Innovation With Domain-Expert AI Agents!

Reaction liquid for detecting magnesium ions in blood sample, dry test paper and preparation method of dry test paper

A technology of reaction solution and test paper, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of detection signal interference, inability to eliminate the influence, and affect the accuracy of detection results, etc., to improve accuracy and specificity , avoid the influence of red blood cells, and reduce the effect of ion interference

Pending Publication Date: 2022-07-22
广州万德康科技有限公司
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the test paper with this structure detects the light signal, it cannot eliminate the influence of the red color of the blood cells in the blood filter layer on the detection light, which makes the detection signal interfered and affects the accuracy of the final detection result.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Reaction liquid for detecting magnesium ions in blood sample, dry test paper and preparation method of dry test paper
  • Reaction liquid for detecting magnesium ions in blood sample, dry test paper and preparation method of dry test paper
  • Reaction liquid for detecting magnesium ions in blood sample, dry test paper and preparation method of dry test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] The preparation method of the dry test paper for detecting magnesium ions in the blood sample described in this embodiment includes the following steps:

[0080] Step 1. Preparation of diffusion layer (1660 material): take 0.85g sodium chloride, 7g BSA, 0.1g surfactant Triton-100, use 100mL pure water to stir and dissolve, press 50mL / m 2 Then, after drying at 37°C for 0.5h, it was cut into a diffusion layer of 7mm / stripe and set aside for use.

[0081] Step 2: Prepare the precipitation layer (BTS material): use 1g of surfactant Brij35 to dissolve in 100mL of pure water by stirring, press 50mL / m 2 Then, after drying at 37°C for 0.5h, it was cut into a 10mm / stripe precipitation layer for use.

[0082] Step 3. Preparation of light-shielding layer (3254 material): After stirring and dissolving 100 mL of pure water with 1 g of surfactant Brij35 (dodecyl polyethylene glycol ether), press 20 mL / m 2 The amount of the coating, and then dried at 37 ° C for 0.5h, cut into 10mm / s...

Embodiment 2

[0089] The preparation method of the dry test paper for detecting magnesium ions in the blood sample described in this embodiment includes the following steps:

[0090] Step 1: Preparation of the diffusion layer: the method is carried out as described in Example 1.

[0091] Step 2: Preparing the precipitation layer: carry out the method described in Example 1.

[0092] Step 3: Prepare the light-shielding layer: carry out the method described in Example 1.

[0093] Step 4: Prepare the reaction layer: Weigh the corresponding masses of reagents 1 to 5 in Example 1, add them to 100 mL of 0.5M TES buffer with pH=7.2, and stir for half an hour to dissolve, and it is solution A, which is ready for use; take Table 2.1 The corresponding mass of the intermediate reagents 6-7 was added to 100 mL of 0.5M TES buffer solution with pH=7.2, and stirred for half an hour to dissolve, and it was the B solution, which was ready for use. The reaction layer paper strip of each test strip was trea...

Embodiment 3

[0098] The preparation method of the dry test paper for detecting magnesium ions in the blood sample described in this embodiment includes the following steps:

[0099] Step 1. Carry out the method described in Example 1.

[0100] Step 2. Carry out the method as described in Example 1.

[0101] Step 3: Prepare the light-shielding layer: carry out the method described in Example 1.

[0102] Step 4. Prepare the reaction layer: Weigh the corresponding masses of reagents 1 to 5 in Example 1, add them to 100 mL of 0.5M TES buffer with pH=7.2, and stir for half an hour to dissolve, and it is solution A, which is ready for use; take Table 3.1 The corresponding mass of the intermediate reagents 6-7 was added to 100 mL of 0.5M TES buffer solution with pH=7.2, and stirred for half an hour to dissolve, and it was the B solution, which was ready for use. The reaction layer paper strip of each test strip was treated with 6.5 μL of solution B, and then dried at 37°C for 90 min; after dryi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
pore sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to dry test paper for detecting magnesium ions in blood and a preparation method of the dry test paper. The dry test paper sequentially comprises an upper supporting layer, a diffusion layer, a precipitation layer, a shading layer, a reaction layer and a lower supporting layer, the shading layer is a non-woven fabric coated with a dodecyl polyglycol ether aqueous solution; the reaction layer is treated by a reaction solution, the reaction solution comprises a component A and a component B, the component A comprises 10 KU to 25 KU of peroxidase, 3.6 KU to 10 KU of glycerol-3-phosphate oxidase, 0.3 g to 2 g of glycerol, 2 g to 4 g of adenosine triphosphate and a TES buffer solution of 5 KU to 12 KU of glycerol kinase; and the component B is prepared from 0.04 to 0.1 g of DAOS (Diethyl Orthosilicate) and 0.04 to 0.1 g of a TES (Tetraethyl Sulfonate) buffer solution of 4-AAP (4- The dry test paper disclosed by the invention can reduce the influence of red blood cells to the greatest extent, can directly detect the concentration of magnesium ions in blood, and is accurate in detection result and high in specificity.

Description

technical field [0001] The invention belongs to in vitro detection technology, and relates to a reaction solution for detecting magnesium ions in blood samples, a dry test paper and a preparation method thereof. Background technique [0002] Magnesium is a metal ion that exists widely in living organisms and is an essential ion that affects the activities of various enzymes. It is very important in the three key physiological mechanisms of vasodilation, coagulation and protection of the blood-brain barrier (BBB), which makes the determination of magnesium an important clinical diagnostic item. At present, the methods for the determination of magnesium ions generally include ion selective electrode method, atomic absorption spectrometry (AAS), spectrophotometry, dye colorimetry and so on. Among them, the AAS method has the best specificity and the most accurate, but the instrument is expensive and difficult to automate. The dye colorimetry method is generally established ba...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/48
CPCC12Q1/485
Inventor 李玉宝符美强朱世成
Owner 广州万德康科技有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com