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Supercritical drying of chromatography media

A critical point drying, supercritical fluid technology, applied in the field of supercritical drying of chromatographic media

Pending Publication Date: 2022-07-22
MERCK MILLIPORE LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Other drying methods can only maintain the properties of the film in the dry state for a limited storage time

Method used

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  • Supercritical drying of chromatography media
  • Supercritical drying of chromatography media
  • Supercritical drying of chromatography media

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1- 1

[0151] Example 1 - General Materials and Methods

[0152] Chemicals

[0153] Liquefied carbon dioxide (medical grade 99.99%) was obtained from PraxAir Canada Inc., absolute alcohol reagent (<0.005% water) and calcium chloride were obtained from MilliporeSigma, and buffer solution 1OX PBS liquid concentrate (growing cells) was purchased from VWR.

[0154] protein

[0155] Affinity purified human IgG was obtained from Innovative Research Inc. (Novi, MI, USA).

[0156] Membrane material

[0157] Epoxy resin films are prepared by polymerizing acrylate and or acrylamide monomers and a crosslinking agent within a support web material in a UV-initiated reaction. By introducing suitable polymerizable functional groups into the gel polymerization solution, various functional membranes containing protein-binding groups (eg, ion exchange, hydrophobic interactions, and hydrophilic interactions) can be produced in a single polymerization step. Wet cleaned membranes can also be ...

Embodiment 2

[0191] Example 2 - Comparison of critical point drying epoxy-containing films to other drying methods and their effect on performance during shelf life role

[0192] Different batches of epoxy resin membranes with similar permeability and their bioligand-derived forms with comparable protein binding capacity were dried using a two-step critical point drying method. In the first step, the membrane in roll form is subjected to a water / ethanol exchange process, in which the membrane roll is placed in an exchange solvent of a certain alcohol content for a specified time, the reagents are subsequently decanted, and fresh specified amount of the next step is added amount of alcohol solution, as outlined in Exchange Scheme B (Table 2). In the second step, the ethanol-wetted film roll was placed in a critical point drying instrument chamber (Leica CPD300) and subjected to a purging process for liquid CO 2 Instead of ethanol, the drying chamber conditions were subsequently shifted...

Embodiment 3

[0195] Example 3 - Critical point drying of epoxy-containing membranes using different exchange protocols

[0196] In order to obtain a successful drying method using supercritical fluids, water must be removed and replaced with ethanol. Several factors are expected to affect the water removal process: water content in the ethanol exchange solution, exchange time, number of exchange steps, sandwich screen material and final membrane to solution ratio.

[0197] Two different exchange schemes with graded ethanol content were examined. In Scheme A, the membrane is exchanged with an ethanol solution to replace the water in the membrane with alcohol by a five-step exchange scheme using a solution with progressively lower water content to finally achieve exchange with anhydrous ethanol reagent step. In Scheme B, the exchange process was carried out throughout the eight exchange steps to replace water in the membrane with ethanol. The two protocols A and B are summarized in Tabl...

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Abstract

A method for critical point drying of a composite material is disclosed. After the composite material is exposed to the supercritical fluid, the composite material is dried as the supercritical fluid evaporates under reduced pressure. The composite material can be used as a chromatographic separation medium.

Description

[0001] Related applications [0002] This application claims the benefit of priority from US Provisional Patent Application No. 62 / 900,798, filed September 16, 2019; and US Provisional Patent Application No. 62 / 900,155, filed September 13, 2019. Background technique [0003] Supercritical fluid (SCF) technology has found many uses in many fields, especially in pharmaceutical applications such as particle and crystal engineering, where great control over the physicochemical properties of the produced particles and control of crystal formation is highly desirable . Other applications, such as complex particle formation, extraction, coating, and liposome preparation, have found deep interest in SCF technology. [0004] The use of critical point drying (CPD) has also generated interest in several areas where structure needs to be preserved during drying, such as drying biological specimens for scanning electron microscopy methods. In general, compared to conventional drying, CPD...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): F26B3/00B01J20/26B01J20/28B01J20/291C07K1/16F26B21/14
CPCF26B21/14F26B3/00C07K1/16B01J20/291B01J20/28033B01J20/267C07K1/22B01J20/28057B01J20/28078Y02C20/40B01D15/08B01J20/2808B01J20/28083B01J20/305B01J2220/4856F26B3/02F26B21/10
Inventor J·博伊尔G·斯卡亚A·拉赫布
Owner MERCK MILLIPORE LTD
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