Preparation method and application of tubular hydrogel with asymmetric structure
An asymmetric structure, hydrogel technology, applied in non-active ingredients medical preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problem of being unsuitable for local release of biologically active substances The effect of rich toxicity, rich source and good biocompatibility
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] Preparation of tubular hydrogels with asymmetric structures such as figure 1 shown, the specific steps are as follows:
[0030] S1. Build a glass capillary coaxial device:
[0031] The glass capillary coaxial device includes an inner phase capillary with an outer diameter of 1.5mm and an outer phase capillary with an inner diameter of 2.5mm; the opening of the inner phase capillary is blocked, and it is repeatedly immersed in a concentration of 15% (w / v) two In the ethanol solution of silica nanoparticles for 10 times, a tubular colloidal crystal template is obtained on the surface of the inner phase capillary. The number of particle solutions is adjusted; the inner phase capillary and the tubular colloidal crystal template pass through the axis of the outer phase capillary and are fixed with a mixed hardener.
[0032] S2. Preparation of asymmetric structural hydrogels:
[0033] Prepare a hydrogel solution of 20% (w / v) methacrylated gelatin and 1% (v / v) photoinitiato...
Embodiment 2
[0038] Preparation of tubular hydrogels with asymmetric structures such as figure 1 shown, the specific steps are as follows:
[0039] S1. Build a glass capillary coaxial device:
[0040] The glass capillary coaxial device includes an inner phase capillary with an outer diameter of 1.5mm and an outer phase capillary with an inner diameter of 2.5mm; the opening of the inner phase capillary is blocked, and it is repeatedly immersed in a concentration of 15% (w / v) two In the ethanol solution of silica nanoparticles for 10 times, a tubular colloidal crystal template is obtained on the surface of the inner phase capillary. The number of particle solutions is adjusted; the inner phase capillary and the tubular colloidal crystal template pass through the axis of the outer phase capillary and are fixed with a mixed hardener.
[0041] S2. Preparation of asymmetric structural hydrogels:
[0042] 20% (w / v) methacrylated gelatin (GelMA) and 20% (w / v) silk fibroin aqueous solution were ...
Embodiment 3
[0047] In vitro NGF release experiments:
[0048] In Example 1 or Example 2, the NGF-loaded tubular hydrogel was placed in a well plate containing 1 mL of PBS buffer, and 200 μL of the release solution was taken out at regular intervals into a centrifuge tube, and an equal volume was added at the same time. of fresh PBS buffer was supplemented to 1 mL in the well plate. The released solution was stored at -20°C. like image 3 As shown, the drug release amount was measured by ELISA (enzyme-linked immunosorbent assay) kit after the collection of all time points, and the cumulative release curve of NGF was drawn by the standard curve method.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More