Artificial aggregate and preparation method thereof
A technology of artificial bone and scaffold material, applied in the fields of additive processing, medical science, prosthesis, etc., to achieve good application prospects, good three-dimensional performance, and good effect of promoting bone growth
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[0031] A first aspect of the embodiments of the present application provides a method for preparing an artificial aggregate, such as figure 1 As shown, the preparation method comprises the following steps:
[0032] S01: Establish a degradable support material with a frame shear wall structure;
[0033] S02: prepare exosome microsphere suspension;
[0034] S01: Place the degradable scaffold material in the exosome microsphere suspension, and then perform defoaming treatment and freeze-drying treatment in sequence to obtain artificial bones.
[0035] The preparation method of artificial bone material provided in this example is obtained by placing a degradable scaffold material with a frame shear wall structure in an exosome microsphere suspension and performing defoaming treatment and freeze-drying treatment in sequence, and the degradable scaffold is obtained. The material is based on the characteristics of "frame shear wall structure", so that the artificial bone has good l...
Embodiment 1
[0050] Design and Manufacture of Degradable Support Materials for Concrete "Framed Shear Wall Structures"
[0051] According to the structural characteristics of "frame shear wall structure" (such as figure 2 , the diameter of the first beam column is 500 μm, the diameter of the second beam column is 800 μm, and the diameter of the third beam column is 2000 μm; and the size of the hole enclosed by the adjacent first transverse column and the first longitudinal column is 500 μm, the adjacent The size of the hole enclosed by the second transverse column and the second longitudinal column is 3000 μm, and the size of the hole enclosed by the adjacent third transverse column and the third longitudinal column is 6000 μm μm. ), using computer design to establish a mathematical model of the scaffold material, adding PLGA and TCP to the 3D bioprinter nozzle, using 3D technology to precisely control the various parameters and variables of the micro-spatial structure to match each other...
Embodiment 2
[0053] Preparation of artificial aggregates
[0054] 1. Establishment of exosome sustained-release microspheres
[0055] Dissolve chitosan in deionized water (1:1000, v / v) to prepare 10 ml of chitosan suspension, add 100 μl of mouse bone marrow mesenchymal stem cell BMSCs-derived exosomes at room temperature, mix well, and put into low pressure In the constant temperature nozzle (4°C), it sprays mist-like particles in a low temperature environment (-20°C), and the spray condenses into granular microspheres of different sizes after being sprayed from the nozzle. The microspheres were then sieved twice (2000 mesh and 5000 mesh) respectively to obtain exosome sustained-release microspheres with a diameter in the range of 2-6 μm.
[0056] The cartilage collagen matrix was extracted from rat tail, and dissolved with deionized water (4%, m / v) by stirring to obtain 95 ml of collagen matrix solution. Disperse the prepared exosome sustained-release microspheres in deionization (1:100...
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