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Glucosidic bond connection mode analysis database and construction method and application thereof

A connection method and construction method technology, which is applied in the field of methylation analysis of glycoside compounds in traditional Chinese medicine, can solve the problems of inability to characterize glycosidic bond connection methods, poor sensitivity, and high requirements for analytical instruments, so as to solve the problem of inability to qualitatively analyze uronic acid and The effects of missing standard substances, improving accuracy, and broad application prospects

Pending Publication Date: 2022-08-05
梁军
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Problems solved by technology

As a common platform for polysaccharide structure analysis, gas chromatography-mass spectrometry (GC / MS) has poor sensitivity, requires carbohydrates to be highly volatile, and requires long-term operation to achieve isomer separation
Other analysis methods mainly include anion exchange chromatography, capillary electrophoresis and high performance liquid chromatography. However, due to the diversity and structural similarity of glycosidic bond connection methods, the requirements for analytical instruments are relatively high. complete characterization

Method used

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  • Glucosidic bond connection mode analysis database and construction method and application thereof
  • Glucosidic bond connection mode analysis database and construction method and application thereof
  • Glucosidic bond connection mode analysis database and construction method and application thereof

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Effect test

Embodiment 1

[0061] The construction of embodiment 1 glycosidic bond connection mode analysis database

[0062] 1. Instruments and Reagents

[0063] Acquity UPLC H-Class (Waters, USA), Sciex 4000Qtrap (AB Sciex, USA); Acquity BEH Shield RP18 chromatographic column (2.1×150 mm, 1.7 μm). ML104 / 02 Electronic Analytical Balance (Mettler Toledo Instruments Shanghai Co., Ltd.); Vortex 3000 Vortex Oscillator (Wiggens, Germany); Mikro200R Centrifuge (Scilogex, USA); KQ-500DB CNC Ultrasonic Cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); iodomethane (Zhengzhou Alpha Chemical Co., Ltd.); dimethyl sulfoxide (Tianjin Xinbote Chemical Co., Ltd.); trifluoroacetic acid (Shanghai McLean Biochemical Technology Co., Ltd.); Hydroxylamine hydrochloride (Tianjin Aopusheng Chemical Co., Ltd.); Acetic anhydride (Tianjin Alpha Biotechnology Co., Ltd.); Pyridine (Tianjin Fuyu Fine Chemical Co., Ltd.).

[0064]2. Test method and test results

[0065] In this example, disaccharide and trisaccharide standard pr...

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Abstract

The invention discloses a glucosidic bond connection mode analysis database and a construction method and application thereof. According to the method disclosed by the invention, partial methylated sugar nitrile acetate derivative products (PMANs) in different glycoside connection modes are separated by adopting an ultra-high performance liquid chromatography-mass spectrometry method; performing qualitative analysis on PMANs in different glycoside connection modes according to the retention time and characteristic ion pair of the derivative in combination with a secondary mass spectrum fragment ion pair to establish a glycosidic bond connection mode analysis database; an analysis database constructed according to retention time and characteristic ion pairs is combined with mass spectrum fragments to carry out comprehensive qualitative identification on the connection positions and sequences of glucosidic bonds in the traditional Chinese medicine glycoside compounds, and the method is successfully applied to analysis of the connection positions and sequences of various sugar residues in the traditional Chinese medicine glycoside compounds. Qualitative analysis of connection of different glycoside of uronic acid can be realized; the method disclosed by the invention has obvious advantages in identification of homologous monosaccharide, isomer monosaccharide and symmetric monosaccharide.

Description

technical field [0001] The invention relates to a glycosidic bond connection mode analysis database and a construction method thereof, in particular to a liquid chromatography tandem mass spectrometry glycosidic bond connection mode analysis database and a construction method, as well as applications in the methylation analysis of traditional Chinese medicine glycoside compounds and the like, belonging to traditional Chinese medicine The field of methylation analysis of glycosides. Background technique [0002] Structural analysis of carbohydrates remains challenging due to the lack of rapid analytical methods that can determine how the glycosidic linkages between various monosaccharides in native oligosaccharides and polysaccharides are linked. Gas chromatography-mass spectrometry (GC / MS), as a common platform for polysaccharide structure analysis, has poor sensitivity, requires high volatility of carbohydrates, and requires long runs to achieve isomer separation. Other an...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/86G01N30/72
CPCG01N30/88G01N30/8696G01N30/7233G01N30/8631
Inventor 夏永刚梁军李野高佳宁
Owner 梁军
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