Epidermal cell growth activating material
A technology of epidermal cells and proliferative activity, applied in the direction of epidermal cells/skin cells, animal cells, vertebrate cells, etc., can solve problems such as difficulties and inability to connect cells
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Embodiment 1
[0077] The raw silk of silkworm was treated with 0.1% Na 2 CO 3 The aqueous solution is boiled for 2 hours under the condition of a solvent ratio of 100 times, and after washing, drying is carried out for degumming.
[0078] Dissolve the spun silk from which sericin has been substantially removed (the content of silk protein is more than 97%) in a solution with a molar ratio of calcium chloride, ethanol, and water of 1:2:8, and then dialyze it in water And desalination, make the aqueous solution of silk protein.
[0079] This aqueous solution was placed in a container for cell culture (made of polystyrene; flat bottom, 35 mm in diameter), and dried at room temperature to form a silk protein film (film-like) on the bottom of the container.
[0080] Next, the cell culture container on which the silk protein film was formed on the bottom was placed at room temperature and a relative humidity of about 90%, thereby adjusting the water content of the silk protein film to about 40%...
Embodiment 2
[0095] In this example, before the control, the wound dressing material was compared with silk protein β-type crystalline powder.
[0096] The α-type and β-type crystalline powders of silk protein were prepared as follows.
[0097] First, cast the silk protein aqueous solution obtained in Example 1 on an acrylic plate and air-dry to form an amorphous film with a thickness of about 30 μm.
[0098] Subsequently, the α-form and β-form were crystallized as follows.
[0099] In the crystallization, gelatinization is placed in a room with room temperature and a relative humidity of about 90% for one day so that the water content of the silk protein film is about 40%.
[0100] β-formation was carried out by spraying 70% ethanol aqueous solution on the silk protein coat.
[0101] These α-type and β-type fibroin coatings are dried, pulverized by physical means, and respectively made into an average particle size of 50 μm or less.
[0102] In order to understand the healing effects o...
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