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Method for preparing titanium dioxide gel film electrochemical biological sensor

A biosensor, titanium dioxide technology, applied in biochemical equipment and methods, microbiological measurement/inspection, scientific instruments, etc., can solve problems such as fixation, achieve the effect of simplifying the preparation process, making the preparation process mild, and avoiding inactivation

Inactive Publication Date: 2005-07-06
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

TiO has not been immobilized in sol-gel by molecular self-assembly 2 membranes, and have been used to immobilize biomolecules as reported

Method used

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  • Method for preparing titanium dioxide gel film electrochemical biological sensor
  • Method for preparing titanium dioxide gel film electrochemical biological sensor
  • Method for preparing titanium dioxide gel film electrochemical biological sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1. Self-assembly forms a titanium dioxide film and prepares a hydrogen peroxide biosensor

[0025] 1. Cut the ITO electrode into a size of 0.5×1.0cm, then put it into the detergent and ultrasonically clean it for 15 minutes twice, then ultrasonically clean it in absolute ethanol for 10 minutes, and finally ultrasonically clean it in secondary water for 15 minutes, and end Afterwards, dry the electrode and let it stand for use.

[0026] 1.1. Soak the ITO electrode in 5mol / L sodium hydroxide solution for 10 hours, take it out, fully rinse it with secondary water, and dry it with nitrogen;

[0027] 1.2. Immerse the electrode in 100mM butyl titanate solution for 3 minutes, the solvent is a mixed solution of ethanol and toluene with a volume ratio of 1:1; then soak the electrode in absolute ethanol for one minute, and finally soak the electrode in secondary water One minute.

[0028] 2. The above-mentioned modified electrode was immersed in 2 mg / mL horseradish p...

Embodiment 2

[0031] Example 2. Self-assembly forms a titanium dioxide film and prepares a hydrogen peroxide biosensor

[0032] 1. Cut the ITO electrode into a size of 0.5×1.0cm, then put it into the detergent and ultrasonically clean it for 15 minutes twice, then ultrasonically clean it in absolute ethanol for 10 minutes, and finally ultrasonically clean it in secondary water for 15 minutes, and end Afterwards, dry the electrode and let it stand for use.

[0033] 1.1. Soak the ITO electrode in 2mol / L sodium hydroxide solution for 12 hours, take it out, fully rinse it with secondary water, and dry it with nitrogen;

[0034] 1.2. Immerse the electrode in 200mM butyl titanate solution for 2 minutes, and the solvent is a mixed solution of ethanol and toluene with a volume ratio of 3:1;

[0035] 1.3. Soak the electrode in absolute ethanol for 3 minutes;

[0036] 1.4. Soak the electrode in secondary water for 3 minutes;

[0037] 2. The above-mentioned modified electrode was immersed in 2 mg / mL ...

Embodiment 3

[0039] Example 3. Self-assembly forms a titanium dioxide film and prepares a hydrogen peroxide biosensor

[0040] The cleaning of the electrode and the process of surface -OH are the same as in Example 1. After -OH, the electrode can be immersed in 10% silane coupling agent KH550 aqueous solution for 25 minutes to make the surface of the electrode -NH 2 All the other steps are the same as in Example 1.

[0041] The performance stability of the obtained biosensor is better, and it will not be inactivated after being stored for one month. The choice of experimental conditions is the same as

[0042] Example 1.

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Abstract

A method for preparing a titanium dioxide gel film electrochemical biosensor, which mainly consists of the following steps: Step 1, preparing TiO 2 Membrane: Soak the ITO electrode in 2-8mol / L sodium hydroxide solution for 5-12 hours, then immerse the electrode in 50-300mM butyl titanate solution for 2-5 minutes, then put the electrode into absolute ethanol Soak in water for 1-3 minutes, finally soak the electrode obtained in step 1.3 in secondary water for 1-3 minutes, step 2, immerse the electrode obtained in step 1 in the aqueous solution of enzyme (concentration: 2-4 mg / mL) for 30-120 A titanium dioxide gel membrane electrochemical biosensor is fabricated in minutes. The sensor prepared by the method of the present invention can reach the maximum response within 10s, and the detection limit of detecting hydrogen peroxide reaches 10 -6 mol / L, detection linear range is 2×10 -6 mol / L~3×10 -3 mol / L, and the sensor can be stored for 14 days without losing activity and can be reused.

Description

1. Technical field [0001] The invention relates to an electrochemical biosensor prepared by preparing a titanium dioxide gel film and immobilizing bioactive substances on the electrode surface by a self-assembly method. 2. Background technology [0002] Biosensor is one of the frontier fields of current biology and analytical chemistry research. Biosensors use highly selective enzymes, antibodies, antigens and other biologically active substances as receptors to perform highly selective and sensitive analysis and detection of target analytes. In the development of sensors, the immobilization of biological substances on the support surface is crucial. [0003] The current immobilization methods of biological substances on the carrier surface include adsorption method, covalent bonding method, cross-linking method and embedding method. Adsorption is a simple immobilization method, usually through the action of electrostatic attraction to immobilize biological substances. Thi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/26G01N27/327
Inventor 朱俊杰傅瑞玲徐锦忠陈洪渊
Owner NANJING UNIV
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