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Myocardium peptide and its use

A technology of cardiopeptin and copeptin, which is applied in the field of biochemistry, can solve the problems of ingredients, uses and effects not specified in detail, and achieve the effect of short working time, high activity and high concentration

Inactive Publication Date: 2006-05-10
大连珍奥药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] However, the above-mentioned patented technology only roughly separates, purifies, and conducts a simple activity test on the bioactive peptide, without specifying its specific components, uses, and effects.

Method used

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  • Myocardium peptide and its use
  • Myocardium peptide and its use
  • Myocardium peptide and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] This experimental example involves the physical and chemical properties, purity, content and activity of cardiopeptin solution

[0063] 1. Physicochemical properties, purity and content of cardiopeptin

[0064] Cardiopeptin of the present invention is a small molecular active polypeptide, stable in biological activity within the pH range of 3-8, sensitive to proteinase K, unchanged in biological activity at 85°C for 10 minutes, and stable under freezing or freeze-drying conditions; analyzed by HPLC, The weight average molecular weight is less than 10000Da (see Figure 1), preferably the weight average molecular weight is 2000-8000Da.

[0065] Cardiopeptin activity OD value (x±s)

[0066] Note: ** Compared with the injured group ** P<0.01

[0067] It can be seen from Table 1 that the biological activity of cardiopeptin is stable within the pH range of 3-8.

[0068] According to FPLC analysis, cardiopeptin mainly has 5 component peaks, and the sum of the rela...

Embodiment 2

[0170] The present invention has carried out the main pharmacodynamics test to described cardiopeptin, observed cardiopeptin on cardiac muscle morphological index, physiological index and biochemical index on whole body and isolated myocardial ischemia and ischemia-reperfusion model. and its effect on myocardial oxygen consumption. The research results are as follows:

[0171] 1. Effect of cardiopeptin on myocardial ultrastructural damage caused by ischemia-reperfusion

[0172] According to the method of reference literature, after ligation of coronary artery LAD in rats for 5 minutes, cardiopeptin or control drug was injected sublingually, the ligation thread was loosened after 10 minutes of myocardial ischemia, reperfusion was performed for 30 minutes, and lead II ECG was recorded at the same time. After reperfusion, blood was collected from the abdominal aorta, the heart was taken out, rinsed with normal saline through the aorta perfusion, then perfused with 6% glutaraldeh...

Embodiment 3

[0216] Take 1 kg of healthy suckling pig ventricular muscle, wash and chop, add 1 kg of sterilized distilled water to homogenize at a speed of 3000rpm / min, freeze the homogenate at -20°C for 24 hours, repeat 3 times after thawing, and heat to 75°C , filter and remove residue with a plate and frame filter (purchased from Guangzhou Institute of Medical Devices) of model XAS03-172 / 8, select 10u medium speed filter paper for use, obtain the crude filtrate, and the hollow fiber column (the specification is F60, purchased from Switzerland) for the crude filtrate Jinbao Company) ultrafiltration, obtaining molecular weight is 12Kd fine filtrate, with ultrafiltration membrane (the specification is 10Kd, Millipore Company) ultrafiltration, molecular weight cut-off is 150ml of cardiopeptin solution of 9500Da, concentrates product with the reverse osmosis concentration column of Millipore Company.

[0217] The solution of the cardiopeptin was checked to meet the quality standard, then filt...

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Abstract

A myocardial peptide, which is a polypeptide extracted from the heart of mammal other othan man and contains polypeptide, free amino acid, RNA and DNA, the method for testing its bioactivity, and its application in preparing the medicines for treating cardiovascular disease and reparing the damaged cardiac muscle are disclosed.

Description

technical field [0001] The present invention relates to a kind of cardiopeptin and its application, specifically, the present invention relates to a kind of cardiopeptin extracted from the heart of a healthy mammal other than human beings and its application, and belongs to the field of biochemical technology. Background technique [0002] "Myocardial protection" has been a hot spot in the research of heart surgery and heart surgery in recent years. Recent data show that many changes occur in ischemic and hypoxic cardiomyocytes, including intracellular calcium overload, free radical production, membrane damage, ATP (adenosine triphosphate, ATP) level decline, oxygen depletion and so on. [0003] The improvement and popularization of cardiac surgery has relieved the pain of many patients and improved people's quality of life. With the continuous improvement of people's requirements for myocardial protection, basic and clinical research has become more and more in-depth. Myo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61K35/34A61K9/10
Inventor 孔祥平万华印李茹冰杨联萍曾平鲁李恕王日升梁强阮忠生
Owner 大连珍奥药业股份有限公司
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