Organic fertilizer and its preparing method
A technology of organic fertilizer and bacterial strain, applied in the field of organic fertilizer and its preparation, to achieve the effects of less one-time investment, enhanced air permeability and growth promotion
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Embodiment 1
[0030] Mix 200 grams of potatoes (peeled), 20 grams of glucose, 18 grams of agar, and 1000 milliliters of water to adjust the pH to 7.4 to prepare a potato dextrose agar medium.
[0031] Inoculate Potato Dextrose Agar Medium with Thinporus CFCC 7005 and Saccharomyces cerevisiae AS 2.23, put the inoculated medium into a fermenter for fermentation, the temperature is controlled at 28°C, the ratio of aeration volume to liquid volume is 1.0, and the stirring speed is 250 revs / min, fermentation time is 24 hours, obtains strain liquid, and it contains 2,500,000,000 strains per milliliter.
[0032] Cool the black liquor obtained by using wheat straw as a raw material and adopting the pre-soaking cooking pulping process to 40°C, add the seed liquid according to 1 / 40 of the black liquor volume, stir well, and after mixing evenly, carry out acidification for 5 days, so that The pH value of the black liquor drops to 9.5. At this time, the color of the black liquor changes from dark black...
Embodiment 2
[0034] Mix 200 grams of potatoes, 20 grams of glucose, 3 grams of potassium dihydrogen phosphate, 1.5 grams of magnesium sulfate, 10.05 mg of vitamin B, 20 grams of agar, and 1000 ml of water to prepare a potato comprehensive medium.
[0035] Inoculate the potato comprehensive culture medium with Chimosia chinensis CFCC 7126 and Saccharomyces cerevisiae AS 2.24, put the inoculated medium into the fermenter for fermentation, the temperature is controlled at 31°C, the ratio of aeration volume to liquid volume is 0.5, and the stirring speed is 150 rev / min, the fermentation time is 36 hours, and the strain liquid is obtained, which contains 1.5 billion strains per milliliter.
[0036] Cool the black liquor obtained by using wheat straw as a raw material and adopting the pre-soaking cooking pulping process to 35°C, add the bacterial seed liquid according to the amount of 1 / 35 of the black liquor volume, stir well, and after mixing evenly, carry out acidification for 10 days, so that...
Embodiment 3
[0038] 200 grams of potatoes, 20 grams of glucose, 1.5 grams of yeast extract, 20 grams of agar, and 1000 ml of water were mixed to prepare a potato yeast extract medium.
[0039] Inoculate the potato yeast extract medium with Pleiospora cellar CFCC 7026, Versicolor versicolor CFCC 7032 and Saccharomyces cerevisiae AS 2.240 and AS2.241, and put the inoculated medium into a fermenter for fermentation, and the temperature is controlled at 29°C , the ratio of ventilation rate to liquid volume is 0.8, the stirring speed is 200 rev / min, and the fermentation time is 30 hours to obtain the strain liquid, which contains 2.05 billion strains per milliliter.
[0040] Cool the black liquor obtained by using wheat straw as a raw material and adopting the pre-soaking cooking pulping process to 38 ° C, add the bacterial seed liquid according to the amount of 1 / 37 of the black liquor volume, stir well, and after mixing evenly, carry out acidification for 7 days, so that The pH value of the b...
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