HEV antigenic peptide and its method

A technology of antigens and antibodies, applied in chemical instruments and methods, botany equipment and methods, biochemical equipment and methods, etc., can solve problems such as difficulties in breeding viruses
CN1391579AInactive Publication Date: 2003-01-15BEIJING WANTAI BIOLOGICAL PHARMACY ENTERPRISE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING WANTAI BIOLOGICAL PHARMACY ENTERPRISE
Publication Date
2003-01-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

A highly immunoreactive viral peptide, pE2, is disclosed which is derived from the carboxy-terminal end region of ORF2 region of the hepatitis E virus (HEV) genome. A unique feature of the novel pE2 peptide is that it possesses conformational antigenic determinants which are only exposed when monomers of the peptide associate with one another through non-covalent interactions to naturally form homodimers. The novel pE2 peptide is proven to be highly reactive with sera from patients having current or past infection with HEV which suggests that the homodimer may mimic certain structural features of the HEV capsid protein. Furthermore, the antigenic activity of the pE2 peptide is strictly conformational in nature and therefore, exhibits immunochemical reactivity only when the peptide exists in a dimeric form. Consequently, the antigenic activity is lost upon dissociation of the dimers, but the activity is restored when the monomers reassociate to form dimers. Moreover, diagnostic methods useful in detecting and diagnosing HEV infection, and the use of a vaccine composition effective in preventing hepatitis E virus infection in which the novel pE2 peptide is utilized are also disclosed.
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Description

[0001] The present invention relates to a highly immunoreactive viral peptide pE2 cloned from the genome of a Chinese strain of hepatitis E virus (HEV), and its use in the development of reliable diagnostic methods for the detection and diagnosis of HEV, and in the prevention of human Application of HEV vaccine composition. Background of the invention

[0002] Hepatitis E virus (HEV), first discovered in 1983, causes enterically transmitted hepatitis (Balayan et al., 1983). In 1991, the full-length genome of the virus was cloned and sequenced for the first time, and it was found to be a single-stranded positive sense non-enveloped RNA (Tam et al., 1991). Although HEV is morphologically similar to members of the Caliciviridae family (Bradley et al., 1988; Huang et al., 1992; Panda et al., 1989), it has a unique genomic organization (Berke et al., 1997). Based on sequence analysis, it is speculated that the 7.2 kb viral genome contains three open reading fram...

Claims

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