No-separation infrared spectrum method of extracting multi-stage microscopic fingerprint to identify Chinese medicine injection

Abstract

The present invention belongs to the field of Chinese medicine detection technology, and includes the steps of: cooling and eliminating solvent from the sample; pressing potassium bromide tablet sample; determining the common infrared spectrum, scattered reflection near infrared spectrum, scattered reflection middle infrared sectrum, reflection spectrum and attenuating total reflection spectrum of the tablet sample; calculating and drawing second derivative spectrogram; determining the 2D relative infrared spectrum and stepped comparison of corresponding spectrogram. The said process identifies Chinese medicine injection and measures its quality without separation and is nn-destructive, fast, simple and intuitive.

Description

technical field [0001] The invention relates to a non-separation extraction spectral analysis identification and identification method of a mixture solution with extremely complex components, in particular to a non-separation extraction infrared spectrum non-separation extraction multi-stage macroscopic fingerprint identification method of a traditional Chinese medicine injection, which belongs to the technical field of traditional Chinese medicine detection. Background technique [0002] The quality control of traditional Chinese medicine is the basis and key to promote traditional Chinese medicine and Chinese medicine. Traditional Chinese medicine, including traditional Chinese medicine injections, is an extremely complex mixture system. In October 2000, the State Administration of Traditional Chinese Medicine issued the "Technical Requirements for Research on Fingerprints of Traditional Chinese Medicine Injections (Provisional)" to guide and standardize the research on fi...

AI Technical Summary

Problems solved by technology

This is an ideal scheme, but at present this method can only be partially realized because of the following difficulties: (1) the conditions of chromatographic separation need to be carefully studied: when there is a surfactant in the system, the separation process often becomes difficult. complication

(2) For HPLC, if the separation conditions are not good, similar substances cannot be separated. For mass spectrometry, the mass of the compound can be obtained, but its structure may not be determined

(3) Sometimes the isolates cannot be detected: mass spectrometry needs to ionize the molecules to be measured, and if some molecules are inconvenient to ionize, it is not easy to detect

(4) The LC separation method takes a long time, and the experimental operating conditions are very harsh. It requires a specific chromatographic column, a specific mobile phase, and the price of the instrument and chromatographic column is relatively expensive, and the maintenance cost is high.

It has two problems, which still cannot be satisfactorily resolved

a. The effectiveness of active ingredients or index ingredients, that is, some controllable indicators are not effective ingredients; b. Since the actual ingredients are far more than three, the concept of overall analysis is lost

(6) Raw materials with imperfect standards and lack of quality standards: the isolated compounds cannot be determined one by one at present, especially when their content is determined, a standard for comparison is usually required

However, there are very few compounds that can be truly determined at present, and it is even more difficult and impractical to obtain standard compounds with full components, so it is inconvenient to promote

(7) The separation process of LC method will lose the originality and compatibility of the analysis system

(8) As far as the current quality control level of existing Chinese medicines is concerned, the production, harvesting, and processing of Chinese medicinal materials have not been standardized, that is, the source of Chinese medicinal materials cannot be strictly controlled, which directly affects the subsequent production. Standardization of Chromatographic Fingerprint of Traditional Chinese Medicine Injection

[0004] The commonly used ultraviolet (UV) spectroscopy method has the following problems: (1) the content determination method is not scientific: for example, rutin is used as a representative of flavonoids to calculate the total flavonoid content, and even non-flavonoid chlorogenic acid is used as a contrast Mistake in Determination of Total Flavonoids Content

(2) Lack of content determination: Since there is no specification of what species the content control index is, only the ultraviolet light absorption rate of a certain wavelength is used as the index, so the specificity is not strong

Therefore, to a certain extent, the original nature and compatibility of the system are destroyed

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