Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fowl castration vaccine gene and recombinant gene vector

A poultry and vaccine technology, applied in the high-tech field of biotechnology, can solve the problems of low expression yield, high cost, complicated purification process, etc., and achieve the effects of low cost and simple production

Inactive Publication Date: 2003-08-20
成都瑞盛高科技股份有限公司
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Prompting animals to produce anti-LHRH antibodies by immunization to neutralize the physiological effects of LHRH, thereby inhibiting the development of gonads to achieve the purpose of castration, its feasibility has been confirmed by many scholars at home and abroad, but most of the research has focused on the synthesis of Peptide vaccines, although the immune effect is remarkable, cannot be put into practical application due to the high cost
Wang Caiping expressed the LHRH / HBsAg fusion protein in the silkworm baculovirus, but it was not popularized and applied due to the low expression yield and cumbersome purification process

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fowl castration vaccine gene and recombinant gene vector
  • Fowl castration vaccine gene and recombinant gene vector
  • Fowl castration vaccine gene and recombinant gene vector

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0061] 1. Design and artificial synthesis of LHRH gene

[0062] Design genes according to the amino acid sequence of LHRH in birds and mammals, and add

[0063] Add NcoI and XhoI restriction sites and protective bases, and use an automatic DNA synthesizer to synthesize the gene.

[0064] 1.1. Livestock vaccine LHRH gene sequence - L1:

[0065] 5′-GG CCATGG CTGAGCATTGGTCTTATGGCCTGCGCCCAGGT

[0066] NCOI

[0067] TAATGA A CTCGAG CA-3′

[0068] terminator XhoI

[0069] 1.2. Poultry vaccine LHRH gene sequence - L2:

[0070] 5′-GG CCATGG CTGAGCACTGGTCTTACGGCCTGCAGCCGGGT

[0071] NCOI

[0072] TAATGA A C TCGAG CA-3′

[0073] terminator XhoI

[0074] 1.3. LHRH Gene Sequence 1 of General Livestock / Poultry Vaccine - L3:

[0075] 5′-GG CCATGG CTGAACACTGGTCCTACGGTCTGCGTCCGGGT

[0076] NcoIGGCGGTGAGCACTGGTCTTACGGCCTGCAGCCGGGTGGCGGTGAACATTGGTCTTACGGTCTGCGCCCGGGT TAATGA A CTCGAG CA-3′

[0077] terminator XhoI

[0078] 1.4. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The fowl farm animal castration vaccine gene and recombinant gene vector is used for producing farm animal castration gene engineering subunit vaccine and belongs to biological technology. The production process includes artificial synthesis of livestock, fowl or universal LHRH gene segment with NcoI and XhoI enzyme incision sites separately in its 5' end and 3' end; inserting pET-32c plasmid to constitute four kinds of recombinant pET-32c LHRHn / N / X plasmid to express LHRH-Trx fusion protein; and converting colibacillus BL21(DE3) with the recombinant plasmid to produce expression produce soluble protein. The farm animal castration gene engineering subunit vaccine is easy to purity, universal for livestock and fowl, and may be used for immunizing chicken, pig, sheep, rabbit, dog, can and other animal for effective castration.

Description

[0001] This application is a divisional application of application date: 2000.6.23, application number: 00107831.3, name: animal castration vaccine gene and genetic engineering body. (1) Technical field [0002] The animal castration vaccine gene and the recombinant gene carrier of the present invention are used for producing luteinizing hormone releasing hormone (LHRH) genetically engineered subunit vaccines for castration of livestock and poultry, and belong to the high-tech field of biotechnology. (2) Technical background [0003] Luteinizing hormore releasing hormone (LHRH) is a decapeptide hormone secreted by the hypothalamus of animals, and its main biological function is to control the synthesis and secretion of luteinizing hormone (LH) and Follicle hormone (FSH), these hormones can promote the normal development of gonads and sex organs, and maintain the reproductive function of animals. Castration vaccine can immune regulate the level of sex-related hormones in anim...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/7088A61K39/00A61P15/00C07H21/04C12N15/13C12N15/16C12N15/63C12P19/34
Inventor 吉传义杜念兴朱杰青龚光明王凌龚晓明
Owner 成都瑞盛高科技股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com