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Quantitative detection method for transgenic farm product and food mixture

A quantitative detection and genetically modified technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, fermentation, etc., can solve the problems of inability to perform quantitative analysis and quantitative analysis, and achieve the guarantee of inalterability and correctness , The effect of detecting accurate data

Inactive Publication Date: 2003-08-27
HANGZHOU BIOER TECH CO LTD
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AI Technical Summary

Problems solved by technology

[0003] At present, some detection methods related to genetically modified plants or food have appeared on the market. A company in Sichuan has developed a gene detection chip for the identification of genetically modified plants. The DNA sample to be detected is determined by the known oligonucleotide sequence. Hybridization is used to detect samples, which can only make judgments and analyzes of samples, but not quantitative analysis; Dalian TAKARA Company also launched a qualitative PCR detection kit for genetically modified products, which requires agarose gel electrophoresis, and can only identify whether the plant is It is a genetically modified product and cannot be quantitatively analyzed

Method used

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  • Quantitative detection method for transgenic farm product and food mixture
  • Quantitative detection method for transgenic farm product and food mixture

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Embodiment 1

[0039] Embodiment 1: the detected food is corn purchased from the market (assumed to contain the herbicide-resistant BAR gene), and the specific operation method is as follows:

[0040] The first step, the extraction of the maize genome

[0041] Firstly, the corn is ground into powder by grinding method, and then the corn genome DNA is extracted with the method of TAKARA Company Corn Genome Extraction Kit. The specific operation steps are described in detail in the kit manual.

[0042] Step 2: Design primers and probes for detection of marker genes and housekeeping genes

[0043] Obtain herbicide resistance BAR gene sequence number X17220 and maize housekeeping gene invertase gene sequence number U16123 from ncbi genbank, and design primers and MGB probe markers on the quantitative detection marker gene herbicide resistance BAR gene and maize housekeeping gene invertase gene As follows:

[0044] Design primers and probes for the herbicide-resistant BAR gene, the amplified pr...

Embodiment 2

[0090] Embodiment 2: The food detected is the soybean purchased from the market, and the specific operation method is as follows:

[0091] The first step, soybean genome extraction

[0092] Firstly, the soybean is ground into powder by grinding method, and then the soybean genome DNA is extracted by using the soybean genome extraction kit method of TAKARA Company. The specific operation steps are described in detail in the instruction manual of the kit.

[0093] Step 2: Design primers and probes for detection of marker genes and housekeeping genes

[0094] The CAMV35S gene sequence number and the soybean housekeeping gene LECTIN gene sequence number K00821 were obtained from ncbi genbank, and primers and MGB probe markers were designed on the quantitative detection marker gene CAMV35S gene and soybean housekeeping gene LECTIN gene as follows:

[0095] Design CAMV35S gene primers and probes, the product amplified by the following primers ranges from 2343bp to 2404bp in AJ25101...

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Abstract

The invention refers to the ration checking method for transferring gene farm produce and food compound. The method includes folloiwng steps: the extraction of gene group DNA from transferring gene farm produce and food compound; checks the design of sign gene, special gene added ingredient and probe; ascertains the standard curve of ration checking sample; real-time fluorescence ratio polymerase chain reaction (PCR) checking, ration analysis.

Description

Technical field: [0001] The invention relates to a method for quantitative detection of genetically modified agricultural products and food mixtures. Background technique [0002] With the rapid development of biotechnology, by introducing exogenous genes into the genetic genes of natural crops, the original genes can be modified, in order to obtain new crop products that can increase the content of nutrients, improve stress resistance, and other improved and optimized traits. , and further improve the quality and function of its processed food in the corresponding aspects, it has been extensively researched and applied. At the same time, people are increasingly concerned about the safety of these plants and their processed products obtained through transgenic technology. Therefore, the quantitative PCR detection and analysis of the transgenic rate of genetically modified plants or foods by fluorescent quantitative PCR detection of plant genetic mater...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/34C12Q1/68
Inventor 毛立新赵怀汪时贵华灿中陈芝娟封帆杜亚娟
Owner HANGZHOU BIOER TECH CO LTD
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