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Microbial bacteria agent for promoting growth of plant and its preparation method

A technology that promotes plant growth and microbial agents, applied in the field of functional application screening and combination technology, can solve the problems of lack of understanding and research on mutual influence, neglect of resistance to diseases and insect pests, etc., to suppress root pathogens, reduce crop diseases and insect pests, and process the preparation method simple effect

Inactive Publication Date: 2003-09-17
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, in an existing patent related to this aspect, its flora consists of Azotobacer chroococcum, Trichoderma koningii, Bacillus mucilaginosus and Bacillus mucilaginosus. It is simply to mix several functional bacteria after separate cultivation, but there is a lack of understanding and research on the possible interaction of these bacteria, and at the same time, the requirements for resistance to pests and diseases are ignored.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: first these four kinds of microorganisms are cultivated separately, and the number of bacteria in the unit mass is detected, and the number of each gram of brown nitrogen-fixing bacteria is cultivated to reach 5.0×10 8 , the number per gram of Azospirillum reaches 3.75×10 8 , the number per gram of Bacillus megaterium reaches 1.0×10 9 , the number of Streptomyces per gram reached 6.25×10 8 , and then mix the equal masses of the cultured bacterial agents so that the ratio of the number of bacteria in each gram of the mixed bacterial agent reaches 20:15:40:25. The order of mixing is as follows: firstly, the fermentation products of Azotobacter brownus and Streptomyces jingyang are mixed in proportion, then the fermentation products of Azospirillum and Bacillus megaterium are mixed, and finally the two types of mixtures are compounded together to obtain the plant growth-promoting type of microbial agents.

Embodiment 2

[0037] Embodiment 2: by 5%, (percentage by weight) is mixed into by nitrogen, phosphorus, potassium (effective nitrogen content is 5%, effective phosphorus content is 7%, effective Potassium content is 8%) and the inorganic biological compound fertilizer prepared in the compound fertilizer of chicken manure. The obtained inorganic biological compound fertilizer was applied to cucumber planting, and compared with the mixed fertilizer composed of the same amount of chicken manure, nitrogen, phosphorus and potassium. The results show that: when applying inorganic biological compound fertilizer to cucumber, the growth of cucumber seedlings is not as good as that of compound fertilizer in the early growth stage of cucumber, but in the middle stage, cucumber seedlings are better than the latter, especially in the fruit growth stage. Inorganic biological compound fertilizer, cucumbers bear fruit earlier, and the cucumbers obtained are large and uniform, without one big and one small ...

Embodiment 3

[0038] Embodiment 3: two kinds of fertilizers obtained in embodiment 2 are applied in the topdressing of apple trees by the same weight, and the result shows: the apple obtained by applying inorganic biological compound fertilizer has no damage by disease and insect, smooth surface and good taste. However, the taste of apples obtained by applying compound fertilizers is not as good as that of the former, and the damage by diseases and insect pests has increased significantly, and the appearance is not good. At the same time, the former is up to 20% more productive than the latter.

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Abstract

A microbial agent for promoting plant growth is prepared from brown azotobacterium, nitrogen-immobilizing spirillum, Bacillus megaterium and streptomycete through three-class culture of each strain and proportionally mixing. It can promote the absorption of plant to mineral elements in soil and suppress the root pathogens.

Description

technical field [0001] The invention relates to a plant-growth-promoting microbial agent and a preparation method thereof, which belongs to the screening and combination technology for the functional application of microbial strains. Background technique [0002] As early as the end of the 19th century, a commercial inoculant prepared from a single Rhizobium had appeared, and at the same time, it had brought increased crop yield and harvest to a certain extent, and was widely used all over the world. With the development of microbial technology, more functional bacteria have been discovered and utilized by people, and the mutual growth and synergy between various bacteria have been studied in depth, and single functional bacteria are gradually developing towards multifunctional bacteria. Throughout our country's plant growth-promoting microbial agents, most of them are just a few functional strains simply pieced together, and there is little research on the possible interact...

Claims

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Application Information

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IPC IPC(8): A01N63/02
Inventor 张毅民万先凯
Owner TIANJIN UNIV
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