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Lincomycin extracting process with binary extractant including benzyl alcohol and n-octyl alcohol

A technology of lincomycin and n-octanol, which is applied in the field of solvent extraction of antibiotics and the extraction of lincomycin with a benzyl alcohol-n-octanol binary extractant, can solve the problems of low extraction rate, high solvent volatility, and operation problems. The problem of high temperature can improve the operating environment, reduce personnel injury and improve the working environment.

Inactive Publication Date: 2004-09-08
HEBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problems of high solvent volatility, low extraction rate and high operating temperature in the existing technology, thereby providing a solvent with low volatility, low toxicity, high extraction rate and operating temperature close to normal temperature or room temperature Method for extracting lincomycin with benzyl alcohol-n-octanol binary extractant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The concrete processing steps of the method for extracting lincomycin with benzyl alcohol-n-octanol binary extractant are:

[0020] (1) Preparation of binary extractant: at room temperature, benzyl alcohol and n-octanol are mixed in a ratio of 90:10 by volume to obtain a binary extractant of benzyl alcohol-n-octanol;

[0021] (2) Adjust the pH value of the lincomycin fermentation filtrate: add sodium hydroxide solution to the lincomycin fermentation filtrate with a lincomycin content of 3126.50u / ml to make its pH 12;

[0022] (3) Extraction: the above-mentioned benzyl alcohol-n-octanol binary extractant is added to the above-mentioned treated lincomycin fermentation filtrate for extraction, and the volume ratio of lincomycin fermentation filtrate to binary extractant is 7: 3. The extraction temperature is 20°C and the extraction time is 20 minutes to obtain the lincomycin extract.

[0023] The sample of lincomycin raffinate was measured by polarimetry, the titer concen...

Embodiment 2

[0028] (1) Preparation of binary extractant: at room temperature, benzyl alcohol and n-octanol are mixed in a ratio of 60:40 by volume to obtain benzyl alcohol-n-octanol binary extractant;

[0029] (2) Adjust the pH value of the lincomycin fermentation filtrate: add sodium hydroxide solution to the lincomycin fermentation filtrate with a lincomycin content of 3069.92u / ml to make its pH 12;

[0030] (3) Extraction: the above-mentioned benzyl alcohol-n-octanol binary extractant is added to the above-mentioned treated lincomycin fermentation filtrate for extraction, the volume ratio of lincomycin fermentation filtrate to binary extractant is 5:1 , the extraction temperature is 40° C., and the extraction time is 40 minutes to obtain the lincomycin extract.

[0031] Take the lincomycin raffinate sample and measure it with polarimetry, the titer concentration of the raffinate is 166.59u / ml, and the primary extraction rate can reach 94.57%.

[0032] (4) Stripping: Adding a concentra...

Embodiment 3

[0035] (1) Preparation of binary extractant: at room temperature, benzyl alcohol and n-octanol are mixed in a ratio of 75:25 by volume to obtain benzyl alcohol-n-octanol binary extractant;

[0036] (2) Adjust the pH value of the lincomycin fermentation filtrate: add sodium hydroxide solution to the lincomycin fermentation filtrate with a lincomycin content of 3130.69u / ml to make its pH 9.5;

[0037] (3) Extraction: the above-mentioned benzyl alcohol-n-octanol binary extractant is added to the above-mentioned treated lincomycin fermentation filtrate for extraction, and the volume ratio of lincomycin fermentation filtrate to binary extractant is 1: 1. The extraction temperature is 40°C and the extraction time is 30 minutes to obtain the lincomycin extract.

[0038] The sample of lincomycin raffinate was determined by polarimetry, the titer concentration of the raffinate was 203.26u / ml, and the primary extraction rate was 93.57%.

[0039] (4) Stripping: adding a concentration of...

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Abstract

The Lincomycin extracting process includes the steps of mixing benzyl alcohol and n-octyl alcohol in the volume ratio of 1.5-9 in room temperature, extracting fermented and filtered Lincomycin liquid with the binary extractant, back extraction with water solution of hydrochloric acid or sulfuric acid in 0.01-1 M concentration, and further refining of the back extracted liquid to obtain Lincomycin. The binary extractant of the present invention has low toxicity, less environmental pollution, little damage to persons, easy-to-control room temperature operation and improved operation environment. The present invention may be used widely in biomedicine preparing field.

Description

technical field [0001] The invention belongs to the method for solvent extraction of antibiotics in the field of biopharmaceuticals, in particular to a method for extracting lincomycin with benzyl alcohol-n-octanol binary extractant. Background technique [0002] At present, the production process of lincomycin used by various manufacturers in my country is mainly the butanol extraction method. This process was reported in the "Chemistry and Analysis of Microbial Drugs", 1996, and it was proposed by American Mason in 1963. Although this method is still used today, there are disadvantages such as large consumption of extractant, high energy consumption, and poor operating environment. In "Chinese Journal of Antibiotics" 2002, 27 (6): (332-336) in the literature, a kind of "Mixed Alcohol Extraction is Used to Reduce the Process Research of B Component Content in Lincomycin Products" is disclosed in the literature. Using a new type of extractant mixed alcohol (C 8 ~C 10 ) to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/06C07H15/16
Inventor 吴兆亮李瑸郑辉杰张朝正
Owner HEBEI UNIV OF TECH
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