Theanine extraction process

An extraction process, theanine technology, applied in the separation/purification of carboxylic acid amides, organic chemistry, etc., can solve the problems of reduced adsorption performance, poor reproducibility of resin materials, difficult industrial realization, etc., achieving good regeneration performance and easy industrialization. production effect

Active Publication Date: 2004-11-17
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There are following defects in this prior art: the theanine purity of extracting is low (purity is about 20%); The reproducibility of res...

Method used

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  • Theanine extraction process

Examples

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Embodiment 1

[0021] 1) Chitosan flocculation and impurity removal: the residual liquid after extracting tea polyphenols from tea leaves, adjust the pH of the solution to 3.0 with hydrochloric acid, add chitosan at a weight ratio of 20:1 to the solids in the residual liquid at 25°C, and chitosan Dissolve the sugar with 1% acetic acid solution to make 2% chitosan solution, flocculate under low-speed stirring for 1 hour, filter and separate, remove the flocculent precipitate, and obtain the flocculation clear liquid;

[0022] 2) Adsorption and removal of pigments and macromolecular substances: take the flocculation clear liquid, add non-polar macroporous adsorption resin with 10% weight of soluble matter in the clear liquid, stir for 5 minutes, filter and remove the resin to obtain a nearly colorless and transparent adsorption clear liquid;

[0023] 3) Cationic exchange resin adsorption: After the cationic resin 732 is replaced by hydrogen form, balance with pH 3.0, 0.1mol / l citric acid and di...

Embodiment 2

[0030] 1) Chitosan flocculation and impurity removal: the residual liquid after extracting tea polyphenols from tea leaves, adjust the pH of the solution to 3.5 with hydrochloric acid, add chitosan at a weight ratio of 10:1 to the solids in the residual liquid at 35°C, chitosan Dissolve sugar with 2% acetic acid solution first to make 0.5% chitosan solution, flocculate under low-speed stirring for 2 hours, filter and separate, remove flocculent precipitate, and obtain flocculation clear liquid;

[0031] 2) Adsorption and removal of pigments and macromolecular substances: take the flocculation clear liquid, add non-polar macroporous adsorption resin with 20% of the weight of soluble matter in the clear liquid, stir for 10 minutes, filter and remove the resin to obtain a nearly colorless and transparent adsorption clear liquid;

[0032] 3) Cationic exchange resin adsorption: After cationic resin WA-2 is replaced by hydrogen form, balance with pH 3.5, 0.1mol / l citric acid and diso...

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Abstract

A process for extracting theanine comprises the steps of chitosan rouging by flocculation, decolorizing by adsorption, adsorbing by cationic ion-exchange resin, ammonia eluation, decompression concentration, adsorbing and rouging by anion exchange resin, concentration alcohol depositing, and recrystallization. The process by the invention can effectively remove the impurity which influences the exchange adsorption function of the cationic ion-exchange resin.

Description

technical field [0001] The invention belongs to the field of tea deep processing, and in particular relates to an extraction process of theanine. Background technique [0002] The chemical name of theanine is: N-ethyl-γ-amino-L-glutamine; molecular formula: C 7 h 14 N 2 o 3 ;Molecular weight: 174, the molecular structure formula is as follows: [0003] [0004] Theanine is a unique component in tea, accounting for more than 50% of the free amino acids in tea, accounting for 1-2% of the dry weight of tea. Studies have shown that theanine has strong physiological activity, and it has shown outstanding performance in inhibiting neural activity, affecting the metabolism and release of dopamine, a neurotransmitter in the brain, protecting nerve cells, lowering blood pressure, and enhancing the efficacy of anticancer drugs. Strong efficacy. The research results on the physiological activity of theanine have aroused widespread concern for it. T...

Claims

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Application Information

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IPC IPC(8): C07C231/24C07C237/06
Inventor 林智杨勇谭俊峰尹军峰吕海鹏成浩
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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